Exhibit 23.1
CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM
We consent to the incorporation by reference in this Registration Statement on FormS-3 of our report dated March 2, 2020 relating to the financial statements of Kaleido Biosciences, Inc., appearing in the Annual Report on Form10-K of Kaleido Biosciences, Inc. for the year ended December 31, 2019. We also consent to the reference to us under the heading “Experts” in such Registration Statement.
| /s/ Deloitte & Touche LLP |
| Boston, Massachusetts |
| March 2, 2020 |
