| | Assure Neuromonitoring Texas Holdings, LLC | | Texas | | 100% |
| | | | | | |
| | Assure Neuromonitoring Utah, LLC | | Utah | | 100% |
| | | | | | |
| | Assure Neuromonitoring Virginia, LLC | | Virginia | | 100% |
| | | | | | |
Assure Networks, LLC | | | | | | |
- Subsidiaries | | Assure Networks Alabama, LLC | | Alabama | | 100% |
| | | | | | |
| | Assure Networks Arizona, LLC | | Arizona | | 100% |
| | | | | | |
| | Assure Networks Colorado, LLC | | Colorado | | 100% |
| | | | | | |
| | Assure Networks Georgia, LLC | | Georgia | | 100% |
| | | | | | |
| | Assure Networks Kansas, LLC | | Kansas | | 100% |
| | | | | | |
| | Assure Networks Louisiana, LLC | | Louisiana | | 100% |
| | | | | | |
| | Assure Networks Michigan, LLC | | Michigan | | 100% |
| | | | | | |
| | Assure Networks Minnesota LLC | | Minnesota | | 100% |
| | | | | | |
| | Assure Networks Missouri, LLC | | Missouri | | 100% |
| | | | | | |
| | Assure Networks Nebraska, LLC | | Nebraska | | 100% |
| | | | | | |
| | Assure Networks Nevada LLC | | Nevada | | 100% |
| | | | | | |
| | Assure Networks New Jersey, LLC | | New Jersey | | 100% |
| | | | | | |
| | Assure Networks Oklahoma, LLC | | Oklahoma | | 100% |
| | | | | | |
| | Assure Networks Pennsylvania, LLC | | Pennsylvania | | 100% |
| | | | | | |
| | Assure Networks South Carolina, LLC | | South Carolina | | 100% |
| | | | | | |
| | Assure Networks Tennessee LLC | | Tennessee | | 100% |
| | | | | | |
| | Assure Networks Texas, LLC | | Texas | | 100% |
| | | | | | |
| | Assure Networks Texas Holdings, LLC | | Texas | | 100% |
| | | | | | |
| | Assure Networks Texas Holdings II, LLC | | Texas | | 100% |
| | | | | | |
| | Assure Networks Utah, LLC | | Utah | | 100% |
| | | | | | |
| | Assure Networks Virginia, LLC | | Virginia | | 100% |
| | | | | | |
Assure Networks Colorado, LLC | | | | | | |
- Subsidiaries | | DNS Professional Reading, LLC | | Colorado | | 100% |
| | | | | | |
| | Littleton Professional Reading, LLC | | Colorado | | 100% |
| | | | | | |
Assure Networks Louisiana, LLC | | | | | | |
- Subsidiaries | | DNS Louisiana, LLC | | Louisiana | | 100% |
