| Cover | Jul. 30, 2024 |
| Cover [Abstract] | |
| Document Type | 8-K |
| Document Period End Date | Jul. 30, 2024 |
| Entity Registrant Name | NAUTILUS BIOTECHNOLOGY, INC. |
| Entity Incorporation, State or Country Code | DE |
| Entity File Number | 001-39434 |
| Entity Tax Identification Number | 98-1541723 |
| Entity Address, Address Line One | 2701 Eastlake Avenue East |
| Entity Address, City or Town | Seattle |
| Entity Address, State or Province | WA |
| Entity Address, Postal Zip Code | 98102 |
| City Area Code | 206 |
| Local Phone Number | 333-2001 |
| Written Communications | false |
| Soliciting Material | false |
| Pre-commencement Tender Offer | false |
| Pre-commencement Issuer Tender Offer | false |
| Title of 12(b) Security | Common Stock, par value $0.0001 per share |
| Trading Symbol | NAUT |
| Security Exchange Name | NASDAQ |
| Entity Emerging Growth Company | true |
| Entity Ex Transition Period | false |
| Entity Central Index Key | 0001808805 |
| Amendment Flag | false |
