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Exhibit 15.1
CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM
We consent to the incorporation by reference in Registration Statement 333-242129 on Form S-8 of our report dated March 31, 2021, relating to the financial statements of Freeline Therapeutics Holdings plc appearing in this Annual Report on Form 20-F for the year ended December 31, 2020.
/s/ Deloitte LLP
Reading, United Kingdom
March 31, 2021