Exhibit 99.4
June 8, 2020
SteriLumen
50 N. Macquesten Parkway
Mount Vernon, NY 10550
| From: | Matthew Hardwick, PhD |
ResInnova Laboratories
8807 Colesville Rd, 3rd Floor
Silver Spring, MD 20910
Dear Mr. Munn,
The Sterilumen mirror and drain units are estimated to have sufficient ultraviolet light (UV) intensity for a significant virus kill. Over the course of 2 hours, it is estimated that the mirror unit has a fluence of approximately 300 mJ/cm2. The drain unit has an estimated fluence of 36 mJ/cm2 after a 15 min exposure. Enveloped viruses, such as human coronavirus SARS CoV-2 (the virus that causes COVID19) and H1N1 influenza A, require approximately 4 mJ/cm2 for > 4-log kill1. It is anticipated that both units generate enough UV fluence in order to kill human coronaviruses.
Our laboratory tested both the mirror and drain units against H1N1 human influenza A virus. The units demonstrated a greater than 2.91-log (mirror) and a 4.21-log (drain) kill after 2 hours and 15 min, respectively. Please find the corresponding report in the following pages.
Testing against human coronavirus 229E, another enveloped RNA virus and a common surrogate for SARS CoV-2, has begun and results are anticipated in the next few weeks.
Sincerely,
/s/ Matthew Hardwick
Matthew Hardwick, PhD
President/CEO
ResInnova Laboratories
1Darnell, et al. Journal of Virological Methods (2004) 121:85-91
2
June 8, 2020
Antiviral Evaluation
Test Method Details
| Test Method | Modified ISO 18184 |
| Test Organism | H1N1 human influenza A virus |
| Test Solution | Eagles Modified Medium with 2% Fetal Bovine Serum |
| Test Disks | 20mm diameter magnetic stainless steel disk |
| Inoculum Applied to Test Disk | 0.010 mL applied to each disk and spread to within 1mm of edges, then dried at room temperature |
| Recovery Solution | Modified SDLP Buffer |
| Measuring Method of Number of Viable Viral Particles | Dilution Plate Method onto MDCK cells, viral particles were detected by. Presence of viral particles (foci) were determined using an anti-XXX monoclonal antibody. Foci were then counted giving Foci Forming Units (FFU). |
Results
| Modified ASTM E2197: Standard Quantitative Disk Carrier Test Method |
| Number of Replicate Experiments | 1 |
| Average Concentration of Inoculum (FFU/mL) | 3.55E+04 = 4.55 log |
| Average Control Recovery (FFU/mL) | 3.72E+04 = 4.56 log |
| Location | Average Virus
Recovered (FFU/disk) | Average
Log Recovery | Average
Log Reduction (vs
Control) | Average Percent
Reduction (vs
Control) |
| Sink Backsplash | 5 | 0.70 | 4.06 | >99.99% |
| Sink Edge | 72.5 | 1.86 | 2.91 | 99.81% |
| Sink Drain | 135 | 2.13 | 3.00 | 99.90% |
| Drain Pipe 1 | 2.5 | 0.40 | 4.21 | >99.99% |
