Exhibit 23.1
Consent of Independent Registered Public Accounting Firm
We consent to the incorporation by reference in the Registration Statement on Form S-1 filed pursuant to Rule 462(b) of the Securities Act of 1933, of the reference to our firm under the caption“Experts” and to the incorporation by reference of our report dated October 15, 2021 (except for the stock split described in Note 1 and the corporate reorganization described in Note 2, as to which the date is November 1, 2021) with respect to the balance sheet as of October 14, 2021 of IO Biotech, Inc., and our report dated June 2, 2021 (except for the stock split described in Note 2, as to which the date is November 1, 2021) with respect to the financial statements of IO Biotech ApS for the years ended December 31, 2020 and 2019, included in Amendment No. 3 in the Registration Statement (Form S-1 No. 333-260301) and related Prospectus of IO Biotech, Inc. for the registration of shares of its common stock.
/s/ EY Godkendt Revisionspartnerselskab
Copenhagen, Denmark
November 4, 2021
