Janus Henderson Growth And Income Fund (JADGX) DEFA14A Additional proxy soliciting materials

[Janus letterhead]

December 21, 2012

EDGAR Operations Branch
Securities and Exchange Commission
Division of Investment Management
100 F Street, N.E.
Washington, DC 20549-0505

Re: JANUS INVESTMENT FUND (the “Registrant”)
1933 Act File No. 002-34393
1940 Act File No. 811-01879

Dear Sir or Madam:

On behalf of the Registrant, enclosed please find soliciting material pursuant to Rule 14a-12 relating to the proxy statement filed with the Securities and Exchange Commission on December 19, 2012.

No fee is required in connection with this filing pursuant to Rule 14a-6 under the Securities Exchange Act of 1934.

If you have any questions regarding this filing, please call me at (303) 336-4132.

Respectfully,

/s/ Christine A. Scheel

Christine A. Scheel
Legal Counsel

Enclosure (via EDGAR only)

cc: Stephanie Grauerholz-Lofton, Esq.
Larry Greene, Esq.
Richard C. Noyes, Esq.
Donna Brungardt