Janus Henderson Growth And Income Fund (JADGX) DEFA14A Additional proxy soliciting materials

UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

SCHEDULE 14A

Proxy Statement Pursuant to Section 14(a) of the

Securities Exchange Act of 1934

(Amendment No. )

Filed by the Registrant ☒

Filed by a Party other than the Registrant ☐

Check the appropriate box:

☐ Preliminary Proxy Statement

☐ Confidential, For Use of the Commission Only (as permitted by Rule 14a-6(e)(2))

☐ Definitive Proxy Statement

☐ Definitive Additional Materials

☒ Soliciting Material Pursuant to § 240.14a-12

Janus Investment Fund

(Exact Name of Registrant as Specified in Charter)

151 Detroit Street, Denver, Colorado 80206-4805

(Address of Principal Executive Offices)

303-333-3863

(Registrant’s Telephone No., including Area Code)

Abigail Murray — 151 Detroit Street, Denver, Colorado 80206-4805

(Name and Address of Agent for Service)

Payment of Filing Fee (Check the appropriate box):
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CFS FOR VOTE - OUTBOUND CALL FLOW Hello, I am trying to reach < SH Full Name >. Am I speaking to them? If yes, confirm SH identity Who’s Calling? (prior to confirming SH) My name is < First Name, Last name > and I am calling on a My name is < First name, Last name > and I’m calling on a recorded line regarding your investment in the < Specific Fund recorded line regarding an upcoming shareholder meeting for the Janus Henderson Funds. Name >. < Use Alternate Scripting if appropriate > Is < SH Full Name > available? If a third party Materials were sent to you (via email, if applicable) regarding If yes needs more info the upcoming shareholder meeting and at this time we have not received your vote. The Board is recommending a vote in favor. We are just following up on some materials that were mailed to them. Is If yes / If vote direction is given If No / SH Declines to Vote < SH Full Name > available? What am I voting on? If Households for same SH: < Use Appropriate Rebuttal followed by> I see you have additional investments Would you like to vote along with the with Janus Henderson Funds. recommendations of the Board? Would you like to vote the same way on If no those investments? Vote direction is given SH Declines to Vote after rebuttals Is there a better time to reach them? Thank you. I am recording a < For / Against / Abstain > vote on your Janus Henderson Funds investment(s) and will send a printed confirmation <for each> to < Registration Street address >. < If appropriate > For confirmation purposes, may I have your city, state and zip code? We can be reached at 866-467-3858 and are available < note: if SH declines, we can accept vote with correct zip or city > weekdays between 9am and 11pm EST and between noon and 6pm EST on Saturday. Thank you for your time and your Thank you for your time. Have a great < day / evening >. vote. Have a great < day / evening >. If Households for additional SHs: If yes Thank you. I see that < Other SH Name > also holds this If no investment. May I speak to them?

CTA INBOUND CALL FLOW ïƒ~ May I have the reference number from the letter you received, it’s a seven digit code with letters and numbers starting with zero. Agent Note: Repeat it back (using alpha matches, T as in Tango, B as in Bravo) If Proxy 01 Screen pops or after searching: ïƒ~ May I have your name? ïƒ~ Is the mailing address still < Registration Street Address >? ïƒ~ Is the phone number you called from the best number to reach you at in the future? ïƒ~ This is regarding the investment in the <Company Name>. Use alternate scripting here. ïƒ~ Business Records and Third Parties: Are you authorized to vote on behalf of < Business Name/Shareholder Name >? What is your name and < position with the company / relationship to the shareholder >? We were trying to contact you because your vote is needed for the upcoming shareholder meeting. Would you like to vote along with the recommendations of the board? YES NO Use Appropriate Rebuttal Would you like to vote along with the If HHs for same SH OR Additional records in Search Results for SH: I see you have additional investments with Janus Henderson Funds. recommendations of the board? Shareholder Would you like to vote the same way on those investments? If Asked: What am I voting on? (Open Briefly) Agrees to Vote NO I am recording your < > vote on your Janus Henderson Funds investments Thank you for your time. Have a and will send you a printed confirmation < for each > to (address). For good day/evening! confirmation purposes, may I have the city, state and zip code that we’ll be mailing your confirmation to? < Note: if the SH declines, we can accept the vote with the correct city or zip> (if appropriate – non-CTA) Are there any investments registered differently that you would like to vote today? If Households for additional SHs: Thank you. I see that < Other SH Name > also holds this investment. May I speak to them? <if the phone is passed to a second holder, move to OUTBOUND CALL FLOW> Thank you for your time and your vote. Have a good day/evening!

VOTE TODAY!

Janus Henderson Global Technology and Innovation Fund

Janus Henderson Global Technology and Innovation Portfolio

Janus Henderson Research Fund

Janus Henderson Research Portfolio

Proxy Campaign

Distribution of the Proxy Statement for Janus Henderson Global Technology and Innovation Fund, Janus Henderson Global Technology and Innovation Portfolio, Janus Henderson Research Fund, and Janus Henderson Research Portfolio has commenced to shareholders as of October 22, 2024. Please read the materials carefully and vote your shares. If you have any questions regarding the proposals, or need assistance with voting, you may call Computershare Fund Services, the Fund’s proxy solicitor, toll free at 1-866-467-3858.

Please vote your shares as soon as possible!