Peoples Bancorp (PEBO) 10-Q 2023 Q2 Quarterly report

EXHIBIT 4.1

PEOPLES BANCORP INC. QUARTERLY REPORT ON FORM 10-Q

FOR FISCAL QUARTER ENDED JUNE 30, 2023

PEOPLES BANCORP INC.

138 Putnam Street

Marietta, OH 45750

(740) 373-3155

August 3, 2023

Securities and Exchange Commission

100 F Street, N.E.

Washington, D.C. 20549

RE: Peoples Bancorp Inc. - Quarterly Report on Form 10-Q for the quarterly period ended June 30, 2023

Ladies and Gentlemen:

Peoples Bancorp Inc., an Ohio corporation, is today filing its Quarterly Report on Form 10-Q for the quarterly period ended June 30, 2023 (the “Form 10-Q”), as executed on August 3, 2023.

Pursuant to the provisions of Item 601(b)(4)(iii) of SEC Regulation S-K, Peoples Bancorp Inc. hereby agrees to furnish to the Securities and Exchange Commission, upon request, copies of instruments and agreements defining (i) the rights of holders of Peoples Bancorp Inc.'s long-term debt or (ii) the rights of holders of the long-term debt of one of Peoples Bancorp Inc.'s consolidated subsidiaries, not being filed or incorporated by reference as an exhibit to the Form 10-Q. The long-term debt authorized under such instruments and agreements does not exceed 10% of the total assets of Peoples Bancorp Inc. and its subsidiaries on a consolidated basis. Included among such instruments and agreements are those assumed by Peoples Bancorp Inc. in connection with the merger of Limestone Bancorp, Inc. into Peoples Bancorp Inc. as of the close of business on April 30, 2023.

Very truly yours,

Peoples Bancorp Inc.

/s/ KATIE BAILEY

Katie Bailey

Executive Vice President,

Chief Financial Officer and Treasurer