Exhibit 21.1 Subsidiaries of the Registrant
Registrant: American National Bankshares Inc.
Incorporated under the laws of the Commonwealth of Virginia
Subsidiaries of American National Bankshares Inc.:
American National Bank and Trust Company
Chartered under the laws of the United States
AMNB Statutory Trust I, A Delaware Statutory Trust
MidCarolina Trust I, A Delaware Statutory Trust
MidCarolina Trust II, A Delaware Statutory Trust
