Pressure BioSciences (PBIO) 10-Q 17 May 21 2021 Q1 Quarterly report Financial data

Document and Entity Information

Document and Entity Information - shares	3 Months Ended
	Mar. 31, 2021	May 13, 2021
Cover [Abstract]
Entity Registrant Name	PRESSURE BIOSCIENCES INC
Entity Central Index Key	0000830656
Document Type	10-Q
Document Period End Date	Mar. 31, 2021
Amendment Flag	false
Current Fiscal Year End Date	--12-31
Entity Current Reporting Status	Yes
Entity Interactive Data Current	Yes
Entity Filer Category	Non-accelerated Filer
Entity Small Business Flag	true
Entity Emerging Growth Company	false
Entity Shell Company	false
Entity Common Stock, Shares Outstanding		5,211,612
Document Fiscal Period Focus	Q1
Document Fiscal Year Focus	2021

Consolidated Balance Sheets (Un

Consolidated Balance Sheets (Unaudited) - USD ($)	Mar. 31, 2021	Dec. 31, 2020
CURRENT ASSETS
Cash and cash equivalents	$ 50,530	$ 18,540
Accounts receivable	504,496	131,228
Inventories, net of $342,496 reserve at March 31, 2021 and December 31, 2020	559,365	592,767
Prepaid expenses and other current assets	271,645	314,936
Total current assets	1,386,036	1,057,471
Investment in equity securities	409,098	517,001
Property and equipment, net	14,897	16,490
Right of use asset leases	205,856	221,432
Intangible assets, net	468,750	490,385
TOTAL ASSETS	2,484,637	2,302,779
CURRENT LIABILITIES
Accounts payable	711,332	771,945
Accrued employee compensation	460,280	417,578
Accrued professional fees and other	2,116,468	2,037,806
Other current liabilities	7,490,366	6,330,722
Deferred revenue	41,828	47,328
Convertible debt, net of unamortized discounts of $2,184,810 and $3,948,167, respectively	9,892,777	7,545,670
Other debt, net of unamortized discounts of $2,223 and $0, respectively	1,372,651	1,135,469
Operating lease liability	67,168	65,193
Other related party debt	181,000	166,000
Total current liabilities	22,333,870	18,517,711
LONG TERM LIABILITIES
Long term debt	527,038	527,039
Operating lease liability - long term	138,688	156,239
Deferred revenue	12,404	19,382
TOTAL LIABILITIES	23,012,000	19,220,371
COMMITMENTS AND CONTINGENCIES (Note 4)
STOCKHOLDERS' DEFICIT
Common stock, $.01 par value; 100,000,000 shares authorized; 5,271,707, tie to equity statement and 4,168,324 sharesissued and outstanding on March 31, 2021 and December 31, 2020 respectively	52,717	41,683
Warrants to acquire common stock	30,005,307	29,192,471
Additional paid-in capital	52,860,129	50,312,968
Accumulated deficit	(103,446,609)	(96,465,807)
Total stockholders' deficit	(20,527,363)	(16,917,592)
TOTAL LIABILITIES AND STOCKHOLDERS' DEFICIT	2,484,637	2,302,779
Series D Convertible Preferred Stock [Member]
STOCKHOLDERS' DEFICIT
Convertible Preferred Stock	3	3
Series G Convertible Preferred Stock [Member]
STOCKHOLDERS' DEFICIT
Convertible Preferred Stock	806	806
Series H Convertible Preferred Stock [Member]
STOCKHOLDERS' DEFICIT
Convertible Preferred Stock	100	100
Series H2 Convertible Preferred Stock [Member]
STOCKHOLDERS' DEFICIT
Convertible Preferred Stock
Series J Convertible Preferred Stock [Member]
STOCKHOLDERS' DEFICIT
Convertible Preferred Stock	35	35
Series K Convertible Preferred Stock [Member]
STOCKHOLDERS' DEFICIT
Convertible Preferred Stock	68	68
Series AA Convertible Preferred Stock [Member]
STOCKHOLDERS' DEFICIT
Convertible Preferred Stock	$ 81	$ 81

Consolidated Balance Sheets (_2

Consolidated Balance Sheets (Unaudited) (Parenthetical) - USD ($)	Mar. 31, 2021	Dec. 31, 2020
Inventories reserve	$ 342,496	$ 342,496
Convertible debt, current unamortized discounts	2,184,810	3,948,167
Other debt, unamortized discounts net	$ 2,223	$ 0
Common stock, par value	$ 0.01	$ 0.01
Common stock, shares authorized	100,000,000	100,000,000
Common stock, shares issued	5,271,707	4,168,324
Common stock, shares outstanding	5,271,707	4,168,324
Series D Convertible Preferred Stock [Member]
Convertible preferred stock, par value	$ 0.01	$ 0.01
Convertible preferred stock, authorized	850	850
Convertible preferred stock, shares issued	300	300
Convertible preferred stock, shares outstanding	300	300
Convertible preferred stock, liquidation value	$ 300,000	$ 300,000
Series G Convertible Preferred Stock [Member]
Convertible preferred stock, par value	$ 0.01	$ 0.01
Convertible preferred stock, authorized	240,000	240,000
Convertible preferred stock, shares issued	80,570	80,570
Convertible preferred stock, shares outstanding	80,570	80,570
Series H Convertible Preferred Stock [Member]
Convertible preferred stock, par value	$ 0.01	$ 0.01
Convertible preferred stock, authorized	10,000	10,000
Convertible preferred stock, shares issued	10,000	10,000
Convertible preferred stock, shares outstanding	10,000	10,000
Series H2 Convertible Preferred Stock [Member]
Convertible preferred stock, par value	$ 0.01	$ 0.01
Convertible preferred stock, authorized	21	21
Convertible preferred stock, shares issued	21	21
Convertible preferred stock, shares outstanding	21	21
Series J Convertible Preferred Stock [Member]
Convertible preferred stock, par value	$ 0.01	$ 0.01
Convertible preferred stock, authorized	6,250	6,250
Convertible preferred stock, shares issued	3,458	3,458
Convertible preferred stock, shares outstanding	3,458	3,458
Series K Convertible Preferred Stock [Member]
Convertible preferred stock, par value	$ 0.01	$ 0.01
Convertible preferred stock, authorized	15,000	15,000
Convertible preferred stock, shares issued	6,880	6,880
Convertible preferred stock, shares outstanding	6,880	6,880
Series AA Convertible Preferred Stock [Member]
Convertible preferred stock, par value	$ 0.01	$ 0.01
Convertible preferred stock, authorized	10,000	10,000
Convertible preferred stock, shares issued	8,083	8,043
Convertible preferred stock, shares outstanding	8,083	8,043

Consolidated Statements of Oper

Consolidated Statements of Operations (Unaudited) - USD ($)	3 Months Ended
	Mar. 31, 2021	Mar. 31, 2020
Revenue:
Total revenue	$ 559,874	$ 253,873
Costs and expenses:
Cost of products and services	226,275	175,146
Research and development	299,943	265,690
Selling and marketing	93,328	189,116
General and administrative	1,015,430	1,019,010
Total operating costs and expenses	1,634,976	1,648,962
Operating loss	(1,075,102)	(1,395,089)
Other (expense) income:
Interest expense, net	(4,668,064)	(1,571,800)
Unrealized (loss) gain on investment in equity securities	(107,903)	149,371
Loss on extinguishment of liabilities	(725,159)	(1,136,367)
Other (expense) income	(1,359)
Total other expense	(5,502,485)	(2,558,796)
Net loss	(6,577,587)	(3,953,885)
Deemed dividends on beneficial conversion feature	(57,884)
Preferred stock dividends	(403,215)	(324,586)
Net loss attributable to common stockholders	$ (7,038,686)	$ (4,278,471)
Basic and diluted net loss per share attributable to common stockholders	$ (1.45)	$ (1.62)
Weighted average common stock shares outstanding used in the basic and diluted net loss per share calculation	4,865,826	2,648,039
Products, Services, Other [Member]
Revenue:
Total revenue	$ 559,874	$ 253,873

Consolidated Statements of Cash

Consolidated Statements of Cash Flows (Unaudited) - USD ($)	3 Months Ended
	Mar. 31, 2021	Mar. 31, 2020
CASH FLOWS FROM OPERATING ACTIVITIES:
Net loss	$ (6,577,587)	$ (3,953,885)
Adjustments to reconcile net loss to net cash used in operating activities:
Gain on loan forgiveness	(367,039)
Change in operating lease right-of-use-asset	15,576	17,408
Common stock and warrants issued for interest and extension fees	2,622,078	60,560
Depreciation and amortization	27,190	45,000
Accretion of interest and amortization of debt discount	2,165,780	878,242
Loss on extinguishment of accrued liabilities and debt		635,000
Stock-based compensation expense	61,237	241,769
Loss (gain) on investment in equity securities	107,903	(149,371)
Common stock issued for services	238,512
Changes in operating assets and liabilities:
Accounts receivable	(373,268)	108,472
Inventories	33,402	(38,074)
Prepaid expenses and other assets	43,291	9,699
Accounts payable	(60,613)	54,463
Accrued employee compensation	42,702	(35,885)
Operating lease liability	(15,576)	(17,408)
Deferred revenue and other accrued expenses	799,397	779,371
Net cash used in operating activities	(1,237,015)	(1,364,639)
CASH FLOWS FROM INVESTING ACTIVITIES:
Purchases of property plant and equipment	(3,962)
Net cash used in investing activities	(3,962)
CASH FLOWS FROM FINANCING ACTIVITIES:
Proceeds from issuance of Series AA Convertible Preferred Stock	100,000
Proceeds from stock option exercises	14,773
Net proceeds from convertible debt	730,000	1,865,500
Net proceeds from non-convertible debt - third party	854,538	463,500
Proceeds from non-convertible debt - related party	85,000	8,500
Payments on convertible debt	(191,250)	(520,500)
Payments on non-convertible debt - related party	(70,000)
Payments on non-convertible debt	(250,094)	(450,167)
Net cash provided by financing activities	1,272,967	1,366,833
NET INCREASE IN CASH AND CASH EQUIVALENTS	31,990	2,194
CASH AND CASH EQUIVALENTS AT BEGINNING OF PERIOD	18,540	29,625
CASH AND CASH EQUIVALENTS AT END OF PERIOD	50,530	31,819
SUPPLEMENTAL INFORMATION
Interest paid in cash	37,375	219,224
NON CASH TRANSACTIONS:
Interest added to principal		132,314
Common stock issued for debt settlement		25,000
Common stock issued to settle accrued liabilities		127,855
Discount from warrants issued with debt	162,654	1,205,010
Preferred stock dividends	403,215	324,586
Conversion of debt and interest into common stock	118,000
Discount due to beneficial conversion feature	53,777	404,608
Deemed dividend-beneficial conversion feature	$ 57,884

Consolidated Statements of Chan

Consolidated Statements of Changes in Stockholders' Deficit (Unaudited) - USD ($)	Series D Preferred Stock [Member]	Series G Preferred Stock [Member]	Series H Preferred Stock [Member]	Series H (2) Preferred Stock [Member]	Series J Preferred Stock [Member]	Series K Preferred Stock [Member]	Series AA Preferred Stock [Member]	Common Stock [Member]	Stock Warrants [Member]	Additional Paid-In Capital [Member]	Accumulated Deficit [Member]	Total
Balance at Dec. 31, 2019	$ 3	$ 806	$ 100		$ 35	$ 68	$ 80		$ 25,496	$ 22,599,177	$ (78,942,277)	$ (12,055,407)
Balance, shares at Dec. 31, 2019	300	80,570	10,000	21	3,458	6,880	7,939	2,549,620
Stock-based compensation												241,769
Series AA preferred stock dividend											(324,586)	(324,586)
Series AA preferred stock dividend, shares
Issuance of common stock to settle accrued liabilities									665			127,855
Issuance of common stock to settle accrued liabilities, shares								66,500
Common stock issued for debt settlement									100			25,000
Common stock issued for debt settlement, shares								10,000
Issuance of common stock for debt extension and interest paid-in-kind									385			60,560
Issuance of common stock for debt extension and interest paid-in-kind, shares								38,521
Beneficial conversion feature on debt												404,608
Warrants issued with debt									1,205,010	1,205,010		1,205,010
Warrants issued for debt extension									609,143	609,143		609,143
Net loss											(3,953,885)	(3,953,885)
Balance at Mar. 31, 2020	$ 3	$ 806	$ 100		$ 35	$ 68	$ 80		26,646	24,413,330	(83,220,748)	13,659,933
Balance, shares at Mar. 31, 2020	300	80,570	10,000	21	3,458	6,880	7,939	2,664,641
Balance at Dec. 31, 2020	$ 3	$ 806	$ 100		$ 35	$ 68	$ 81	$ 41,683	29,192,471	50,312,968	(96,465,807)	(16,917,592)
Balance, shares at Dec. 31, 2020	300	80,570	10,000	21	3,458	6,880	8,043	4,168,324
Stock-based compensation										61,237		61,237
Series AA preferred stock dividend											(403,215)	(403,215)
Series AA preferred stock dividend, shares
Beneficial conversion feature on debt										53,777		53,777
Warrants issued with debt									162,654			162,654
Stock option exercise								$ 214		14,559		$ 14,773
Stock option exercise, shares								21,411				21,411
Issuance of warrants for interest paid-in-kind									600,298			$ 600,298
Issuance of common stock for services								$ 1,124		237,388		$ 238,512
Issuance of common stock for services, shares								112,400				112,400
Series AA Preferred Stock offering									49,884	50,116		$ 100,000
Series AA Preferred Stock offering, shares							40
Beneficial conversion option on convertible preferred stock								$ 0		57,884		57,884
Deemed dividend on convertible preferred stock								0		(57,884)		(57,884)
Conversion of debt and interest for common stock								$ 472		117,528		$ 118,000
Conversion of debt and interest for common stock, shares								47,200				47,200
Issuance of common stock for interest paid-in-kind								$ 9,224		2,012,556		$ 2,021,780
Issuance of common stock for interest paid-in-kind, shares								922,372
Net loss											(6,577,587)	(6,577,587)
Balance at Mar. 31, 2021	$ 3	$ 806	$ 100	$ 0	$ 35	$ 68	$ 81	$ 52,717	$ 30,005,307	$ 52,860,129	$ (103,446,609)	$ (20,527,363)
Balance, shares at Mar. 31, 2021	300	80,570	10,000	21	3,458	6,880	8,083	5,271,706

Business Overview, Liquidity an

Business Overview, Liquidity and Management Plans	3 Months Ended
	Mar. 31, 2021
Organization, Consolidation and Presentation of Financial Statements [Abstract]
Business Overview, Liquidity and Management Plans	1) Business Overview, Liquidity and Management Plans Pressure BioSciences, Inc. (“we”, “our”, “the Company”) develops and sells innovative, broadly enabling, pressure-based platform solutions for the worldwide life sciences industry. Our solutions are based on the unique properties of both constant (i.e., static) and alternating (i.e., pressure cycling technology, or “PCT”) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions (e.g., cell lysis, biomolecule extraction) safely and reproducibly. Our primary focus has historically been in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. Additionally, major new market opportunities have emerged in the use of our pressure-based technology expertise in two new platform technology areas: (1) the use of our recently acquired, patented technology from BaroFold, Inc. for gently controlled disaggregation and refolding of biotherapeutic proteins (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) for greatly improved cost-effectiveness, high bioavailability, safer and improved sensory experience in products spanning pharmaceuticals, nutraceuticals, cosmeceuticals, personal care products, agrochemicals, food/beverage and many industrial products and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies.

Going Concern

Going Concern	3 Months Ended
	Mar. 31, 2021
Organization, Consolidation and Presentation of Financial Statements [Abstract]
Going Concern	2) Going Concern The accompanying financial statements have been prepared assuming that the Company will continue as a going concern, which contemplates the realization of assets and the liquidation of liabilities in the normal course of business. However, we have experienced losses from operations and negative cash flows from operations with respect to our pressure cycling technology business since our inception. As of March 31, 2021, we do not have adequate working capital resources to satisfy our current liabilities and as a result, there is substantial doubt regarding our ability to continue as a going concern. We have been successful in raising debt and equity capital in the past and as described in Notes 5 and 6. In addition we raised debt and equity capital after March 31, 2021 as described in Note 7. We have financing efforts in place to continue to raise cash through debt and equity offerings. Although we have successfully completed financings and reduced expenses in the past, we cannot assure you that our plans to address these matters in the future will be successful. These financial statements do not include any adjustments that might result from this uncertainty.

Summary of Significant Accounti

Summary of Significant Accounting Policies	3 Months Ended
	Mar. 31, 2021
Accounting Policies [Abstract]
Summary of Significant Accounting Policies	3) Summary of Significant Accounting Policies Basis of Presentation The unaudited interim financial statements of Pressure BioSciences, Inc. and its consolidated subsidiaries (collectively, the “Company”) included herein have been prepared by the Company in accordance with the instructions to Form 10-Q and the rules and regulations of the U.S. Securities and Exchange Commission. Under these rules and regulations, some information and footnote disclosures normally included in financial statements prepared under accounting principles generally accepted in the United States of America have been shortened or omitted. Management believes that all adjustments necessary for a fair statement of the financial position and the results of operations for the periods shown have been made. All adjustments are normal and recurring. These financial statements should be read together with the Company’s audited financial statements included in its Form 10-K for the fiscal year ended December 31, 2020. Use of Estimates The Company’s consolidated financial statements and accompanying notes are prepared in accordance with accounting principles generally accepted in the United States of America, which require the use of estimates, judgements and assumptions that affect the reported amounts of assets and liabilities at the date of the financial statements and the reported amounts of revenues and expenses during the periods presented. Global concerns about the COVID-19 pandemic have adversely affected, and we expect will continue to adversely affect, our business, financial condition and results of operations including the estimates and assumptions made by management. Significant estimates and assumptions include valuations of share-based awards, investments in equity securities and intangible asset impairment. Actual results could differ from the estimates, and such differences may be material to the Company’s consolidated financial statements. Principles of Consolidation The consolidated financial statements include the accounts of Pressure BioSciences, Inc., and its wholly owned subsidiary PBI BioSeq, Inc. All intercompany accounts and transactions have been eliminated in consolidation. Revenue Recognition We recognize revenue in accordance with FASB ASC 606, Revenue from Contracts with Customers, ASC 340-40, Other Assets and Deferred Costs—Contracts with Customers We identify a performance obligation as distinct if both the following criteria are true: the customer can benefit from the good or service either on its own or together with other resources that are readily available to the customer and the entity’s promise to transfer the good or service to the customer is separately identifiable from other promises in the contract. Determining the standalone selling price (“SSP”) and allocation of consideration from a contract to the individual performance obligations, and the appropriate timing of revenue recognition, is the result of significant qualitative and quantitative judgments. Management considers a variety of factors such as historical sales, usage rates, costs, and expected margin, which may vary over time depending upon the unique facts and circumstances related to each performance obligation in making these estimates. While changes in the allocation of the SSP between performance obligations will not affect the amount of total revenue recognized for a particular contract, any material changes could impact the timing of revenue recognition, which would have a material effect on our financial position and result of operations. This is because the contract consideration is allocated to each performance obligation, delivered or undelivered, at the inception of the contract based on the SSP of each distinct performance obligation. Taxes assessed by a governmental authority that are both imposed on and concurrent with a specific revenue-producing transaction, that are collected by the Company from a customer, are excluded from revenue. Shipping and handling costs associated with outbound freight after control over a product has transferred to a customer are accounted for as a fulfillment cost and are in included in cost of revenues as consistent with treatment in prior periods. Our current Barocycler® instruments require a basic level of instrumentation expertise to set-up for initial operation. To support a favorable first experience for our customers, upon customer request, and for an additional fee, we will send a highly trained technical representative to the customer site to install Barocyclers® that we sell, lease, or rent through our domestic sales force. The installation process includes uncrating and setting up the instrument, followed by introductory user training. Our sales arrangements do not provide our customers with a right of return. Any shipping costs billed to customers are recognized as revenue. The majority of our instrument and consumable contracts contain pricing that is based on the market price for the product at the time of delivery. Our obligations to deliver product volumes are typically satisfied and revenue is recognized when control of the product transfers to our customers. Concurrent with the transfer of control, we typically receive the right to payment for the shipped product and the customer has significant risks and rewards of ownership of the product. Payment terms require customers to pay shortly after delivery and do not contain significant financing components. Revenue from scientific services customers is recognized upon completion of each stage of service as defined in service agreements. We apply ASC 845, “Accounting for Non-Monetary Transactions”, to account for products and services sold through non-cash transactions based on the fair values of the products and services involved, where such values can be determined. Non-cash exchanges would require revenue to be recognized at recorded cost or carrying value of the assets or services sold if any of the following conditions apply: a) The fair value of the asset or service involved is not determinable. b) The transaction is an exchange of a product or property held for sale in the ordinary course of business for a product or property to be sold in the same line of business to facilitate sales to customers other than the parties to the exchange. c) The transaction lacks commercial substance. We recognize revenue for non-cash transactions at recorded cost or carrying value of the assets or services sold. We account for lease agreements of our instruments in accordance with ASC 842, Leases. We record revenue over the life of the lease term, and we record depreciation expense on a straight-line basis over the thirty-six-month estimated useful life of the Barocycler® instrument. The depreciation expense associated with assets under lease agreement is included in the “Cost of PCT products and services” line item in our accompanying consolidated statements of operations. Many of our lease and rental agreements allow the lessee to purchase the instrument at any point during the term of the agreement with partial or full credit for payments previously made. We pay all maintenance costs associated with the instrument during the term of the leases. Deferred revenue represents amounts received from service contracts for which the related revenues have not been recognized because one or more of the revenue recognition criteria have not been met. Revenue from service contracts is recorded ratably over the length of the contract. Disaggregation of revenue In the following table, revenue is disaggregated by primary geographical market, major product line, and timing of revenue recognition. In thousands of US dollars ($) Three Months Ended Primary geographical markets 2021 2020 North America $ 208 $ 143 Europe 84 54 Asia 268 57 $ 560 $ 254 Three Months Ended Major products/services lines 2021 2020 Hardware $ 377 $ 129 Consumables 102 56 Contract research services 6 10 Sample preparation accessories 29 25 Technical support/extended service contracts 24 19 Shipping and handling 19 9 Other 3 6 $ 560 $ 254 Three Months Ended Timing of revenue recognition 2021 2020 Products transferred at a point in time $ 538 $ 225 Services transferred over time 22 29 $ 560 $ 254 Contract balances In thousands of US dollars ($) March 31, 2021 December 31, 2020 Receivables, which are included in ‘Accounts Receivable’ $ 505 $ 131 Contract liabilities (deferred revenue) 54 67 Transaction price allocated to the remaining performance obligations. The following table includes estimated revenue expected to be recognized in the future related to performance obligations that are unsatisfied (or partially unsatisfied) at the end of the reporting period. In thousands of US dollars ($) 2021 2022 Total Extended warranty service $ 42 12 $ 54 All consideration from contracts with customers is included in the amounts presented above. Contract Costs The Company recognizes the incremental costs of obtaining contracts as an expense when incurred if the amortization period of the assets that the Company otherwise would have recognized is one year or less. These costs are included in selling, general, and administrative expenses. The costs to obtain a contract are recorded immediately in the period when the revenue is recognized either upon shipment or installation. The costs to obtain a service contract are considered immaterial when spread over the life of the contract so the Company records the costs immediately upon billing. Concentrations Credit Risk Our financial instruments that potentially subject us to concentrations of credit risk consist primarily of cash, cash equivalents, and trade receivables. We have cash investment policies which, among other things, limit investments to investment-grade securities. We perform ongoing credit evaluations of our customers, and the risk with respect to trade receivables is further mitigated by the fact that many of our customers are government institutions, large pharmaceutical and biotechnology companies, and academic laboratories. The following table illustrates the level of concentration as a percentage of total revenues during the three months ended March 31, 2021 and 2020. For the Three Months Ended March 31, 2021 2020 Top Five Customers 88 % 68 % Federal Agencies 1 % 6 % The following table illustrates the level of concentration as a percentage of net accounts receivable balance as of March 31, 2021 and December 31, 2020. The Top Five Customers category may include federal agency receivable balances if applicable. March 31, 2021 December 31, 2020 Top Five Customers 94 % 89 % Federal Agencies 1 % 10 % Product Supply We utilize a contract assembler for our Barocycler® 2320EXT. They provide us with precision manufacturing services that include management support services to meet our specific application and operational requirements. Among the services provided to us are: ● CNC Machining ● Contract Assembly & Kitting ● Component and Subassembly Design ● Inventory Management ● ISO certification At this time, we believe that outsourcing contract assembly of our Barocycler® 2320EXT is the most cost-effective method for us to obtain ISO Certified, CE and CSA Marked instruments. We currently manufacture and assemble the Barocycler®, HUB440, HUB880, the SHREDDER SG3, and most of our consumables at our South Easton, MA facility. We will regularly reassess the tradeoffs between in-house assembly versus the benefits of outsourced relationships for of the entire Barocycler® product line, and future instruments. Investment in Equity Securities As of March 31, 2021, we held 100,250 shares of common stock of Nexity Global SA, (a Polish publicly traded company). We account for this investment in accordance with ASC 320 “Investments — Debt and Equity Securities”. ASC 320 requires equity investments with readily determinable fair values to be measured at fair value with changes in fair value recognized in net income. As of March 31, 2021, our consolidated balance sheet reflected the fair value, determined on a recurring basis based on Level 1 inputs of our investment in Nexity, to be $409,098. We recorded $107,903 as an unrealized loss during the quarter ended March 31, 2021 for changes in market value. Computation of Loss per Share Basic loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding. Diluted loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding plus additional common shares that would have been outstanding if dilutive potential common shares had been issued. For purposes of this calculation, convertible preferred stock, common stock dividends, and warrants and options to acquire common stock, are all considered common stock equivalents in periods in which they have a dilutive effect and are excluded from this calculation in periods in which these are anti-dilutive to our net loss. The following table illustrates our computation of loss per share for the three months ended March 31, 2021 and 2020: For the Three Months Ended March 31, 2021 2020 Numerator: Net loss attributable to common stockholders $ (7,038,686 ) $ (4,278,471 ) Denominator for basic and diluted loss per share: Weighted average common stock shares outstanding 4,865,826 2,648,039 Loss per common share – basic and diluted $ (1.45 ) $ (1.62 ) The following table presents securities that could potentially dilute basic loss per share in the future. For all periods presented, the potentially dilutive securities were not included in the computation of diluted loss per share because these securities would have been anti-dilutive to our net loss. The Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H and H2 Convertible Preferred Stock, Series J Convertible Preferred Stock, Series K Convertible Preferred Stock, and Series AA Convertible Preferred Stock are presented below as if they were converted into common shares according to the conversion terms. As of March 31, 2021 2020 Stock options 1,358,490 1,393,551 Convertible debt 4,757,701 3,125,633 Common stock warrants 14,901,211 11,295,764 Convertible preferred stock: Series D Convertible Preferred Stock 25,000 25,000 Series G Convertible Preferred Stock 26,857 26,857 Series H Convertible Preferred Stock 33,334 33,334 Series H2 Convertible Preferred Stock 70,000 70,000 Series J Convertible Preferred Stock 115,267 115,267 Series K Convertible Preferred Stock 229,334 229,334 Series AA Convertible Preferred Stock 8,083,000 7,939,000 29,600,194 24,253,740 Accounting for Stock-Based Compensation Expense We maintain equity compensation plans under which incentive stock options and non-qualified stock options are granted to employees, independent members of our Board of Directors and outside consultants. We recognize stock-based compensation expense over the requisite service period using the Black-Scholes formula to estimate the fair value of the stock options on the date of grant. Determining Fair Value of Stock Option Grants Valuation and Amortization Method - The fair value of each option award is estimated on the date of grant using the Black-Scholes pricing model based on certain assumptions. The estimated fair value of employee stock options is amortized to expense using the straight-line method over the vesting period. Expected Term - The Company uses the simplified calculation of expected life, as the Company does not currently have sufficient historical exercise data on which to base an estimate of expected term. Using this method, the expected term is determined using the average of the vesting period and the contractual life of the stock options granted. Expected Volatility - Expected volatility is based on the Company’s historical stock volatility data over the expected term of the award. Risk-Free Interest Rate - The Company bases the risk-free interest rate used in the Black-Scholes valuation method on the implied yield currently available on U.S. Treasury zero-coupon issues with an equivalent remaining term. Forfeitures - The Company records stock-based compensation expense only for those awards that are expected to vest. The Company estimated a forfeiture rate of 5% for awards granted based on historical experience and future expectations of options vesting. The Company used this historical rate as our assumption in calculating future stock-based compensation expense. The Company recognized stock-based compensation expense of $61,237 and $241,769 for the three months ended March 31, 2021 and 2020, respectively. The following table summarizes the effect of this stock-based compensation expense within each of the line items of our costs and expenses within our Consolidated Statements of Operations: For the Three Months Ended March 31, 2021 2020 Cost of sales $ 5,053 $ 7,956 Research and development 25,862 38,826 Selling and marketing 4,595 13,936 General and administrative 25,727 181,051 Total stock-based compensation expense $ 61,237 $ 241,769 Fair Value of Financial Instruments Due to their short maturities, the carrying amounts for cash and cash equivalents, accounts receivable, accounts payable, accrued expenses and debt approximate their fair value. Long-term liabilities include debt and deferred revenue with a carrying value that approximates fair value. Fair Value Measurements The Company follows the guidance of FASB ASC Topic 820, “ Fair Value Measurements and Disclosures The Company generally defines fair value as the price that would be received to sell an asset or paid to transfer a liability in an orderly transaction between market participants at the measurement date (exit price). The Company uses a three-tier fair value hierarchy, which classifies the inputs used in measuring fair values. These tiers include: Level 1, defined as observable inputs such as quoted prices for identical instruments in active markets; Level 2, defined as inputs other than quoted prices in active markets that are either directly or indirectly observable; and Level 3, defined as unobservable inputs in which little or no market data exists, therefore requiring the Company to develop its own assumptions. A slight change in an unobservable input like volatility could have a significant impact on fair value measurement. Financial assets and liabilities are classified in their entirety based on the lowest level of input that is significant to the fair value measurement. The Company has determined that its financial assets are classified within Level 1 in the fair value hierarchy. The development of the unobservable inputs for Level 3 fair value measurements and fair value calculations are the responsibility of the Company’s management. The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of March 31, 2021: Fair value measurements at March 31, 2021 using: March 31, 2021 Quoted prices in active markets (Level 1) Significant other observable inputs (Level 2) Significant unobservable inputs (Level 3) Equity Securities $ 409,098 $ 409,098 - - Total Financial Assets $ 409,098 $ 409,098 $ - $ - The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of December 31, 2020: Fair value measurements at December 31, 2020 using: December 31, 2020 Quoted prices in active markets (Level 1) Significant other observable inputs (Level 2) Significant unobservable inputs (Level 3) Equity Securities 517,001 517,001 - - Total Financial Assets $ 517,001 $ 517,001 $ - $ -

Commitments and Contingencies

Commitments and Contingencies	3 Months Ended
	Mar. 31, 2021
Commitments and Contingencies Disclosure [Abstract]
Commitments and Contingencies	4) Commitments and Contingencies Operating Leases The Company accounts for its leases under ASC 842. The Company has elected to apply the short-term lease exception to leases of one year or less. Our corporate office is currently located at 14 Norfolk Avenue, South Easton, Massachusetts 02375. We are currently paying $6,950 per month, on a lease extension, signed on December 30, 2020, that expires December 31, 2021, for our corporate office. We expanded our space to include offices, warehouse, and a loading dock on the first floor starting May 1, 2017 with a monthly rent increase already reflected in the current payments. We extended our lease for our space in Medford, MA (the “Medford Lease”) from December 30, 2020 to December 30, 2023. The lease requires monthly payments of $7,282 subject to annual cost of living increases. The lease shall be automatically extended for additional three years unless either party terminates at least six months prior to the expiration of the current lease term. The Company accounted for the lease extension of our Medford Lease as a lease modification under ASC 842. At the effective date of modification, the Company recorded an adjustment to the right-of-use asset and lease liability in the amount of $221,432 based on the net present value of lease payments discounted using an estimated borrowing rate of 12%. Following is a schedule by years of future minimum rental payments required under operating leases with initial or remaining non-cancelable lease terms as of March 31, 2021: 2021 $ 128,087 2022 87,383 2023 87,383 Total minimum payments required $ 302,853

Convertible Debt and Other Debt

Convertible Debt and Other Debt	3 Months Ended
	Mar. 31, 2021
Debt Disclosure [Abstract]
Convertible Debt and Other Debt	5) Convertible Debt and Other Debt Convertible Debt On various dates during the three months ended March 31, 2021, the Company issued convertible notes for a total of $860,000 which contained varied terms and conditions as follows: a) 6-12 month maturity date; b) interest rates of 10-18%; c) convertible to the Company’s common stock at issuance at a fixed rate of $2.50. These notes were issued with warrants to purchase common stock that were fair valued at issuance date. The aggregate relative fair value of warrants issued with the notes of $162,654 was recorded as a debt discount to be amortized over the term of the notes. We then computed the effective conversion price of the notes, and recorded a $53,777 beneficial conversion feature as a debt discount to be amortized over the term of the notes. We also evaluated the convertible notes for derivative liability treatment and determined that the notes did not qualify for derivative accounting treatment at March 31, 2021. The specific terms of the convertible notes and outstanding balances as of March 31, 2021 are listed in the tables below. Inception Date Term Loan Amount Outstanding balance with OID Original Issue Discount (OID) Interest Rate Conversion Price Deferred Finance Fees Discount for conversion feature and warrants/shares May 17, 2018 (2) 12 months $ 380,000 $ 166,703 $ 15,200 8 % $ 2.50 $ 15,200 $ 332,407 October 19, 2018 (1) 6 months $ 100,000 $ 100,000 $ - 5 % $ 7.50 $ - $ - November 13, 2018 (1) (3) (4) 6 months $ 200,000 $ 220,000 $ - 5 % $ 2.50 $ - $ 168,634 January 3, 2019 (1)(4) 6 months $ 50,000 $ 50,000 $ 2,500 24 % $ 7.50 $ 2,500 $ - February 21, 2019 (2) 12 months $ 215,000 $ 215,000 $ - 4 % $ 2.50 $ 15,000 $ 107,709 March 18, 2019 (1) 6 months $ 100,000 $ 100,000 $ - 4 % $ 7.50 $ - $ 10,762 June 4, 2019 (2) 9 months $ 500,000 $ 302,484 $ - 8 % $ 2.50 $ 40,500 $ 70,631 June 19, 2019 (2) 12 months $ 105,000 $ 105,000 $ - 4 % $ 2.50 $ 5,000 $ 2,646 June 7, 2019 (1) (4) 6 months $ 125,000 $ 25,000 $ - 5 % $ 7.50 $ - $ 18,254 July 1, 2019 (2) 12 months $ 107,500 $ 107,500 $ - 4 % $ 2.50 $ 7,500 $ 85,791 July 19, 2019 (2) 12 months $ 115,000 $ 115,000 $ - 4 % $ 2.50 $ 5,750 $ 15,460 July 19, 2019 (2) 12 months $ 130,000 $ 130,000 $ - 6 % $ 2.50 $ 6,500 $ - September 27,2019 (2) 12 months $ 78,750 $ 78,750 $ - 4 % $ 2.50 $ 3,750 $ 13,759 October 24, 2019 (2) 12 months $ 78,750 $ 78,750 $ - 4 % $ 2.50 $ 3,750 $ - November 1, 2019 (2) 12 months $ 270,000 $ 270,000 $ - 6 % $ 2.50 $ 13,500 $ - November 15, 2019 (1) 12 months $ 385,000 $ 320,000 $ 35,000 10 % $ 2.50 $ 35,000 $ 90,917 January 2, 2020 (1) 12 months $ 330,000 $ 330,000 $ 30,000 10 % $ 2.50 $ 30,000 $ 91,606 January 24, 2020 (1) 12 months $ 247,500 $ 247,500 $ 22,500 10 % $ 2.50 $ 22,500 $ 89,707 January 29, 2020 (1) 12 months $ 363,000 $ 363,000 $ 33,000 10 % $ 2.50 $ 33,000 $ 297,000 February 12, 2020 (1) 12 months $ 275,000 $ 275,000 $ 25,000 10 % $ 2.50 $ 25,000 $ 225,000 February 19, 2020 (1) 12 months $ 165,000 $ 165,000 $ 15,000 10 % $ 2.50 $ 15,000 $ 135,000 March 11, 2020 (1) 12 months $ 330,000 $ 330,000 $ 30,000 10 % $ 2.50 $ 30,000 $ 232,810 March 13, 2020 (1) 12 months $ 165,000 $ 165,000 $ 15,000 10 % $ 2.50 $ 15,000 $ 60,705 March 26, 2020 (1) 12 months $ 111,100 $ 111,100 $ 10,100 10 % $ 2.50 $ 10,100 $ 90,900 April 8, 2020 (1) 12 months $ 276,100 $ 276,100 $ 25,100 10 % $ 2.50 $ 25,000 $ 221,654 April 17, 2020 (1) 12 months $ 143,750 $ 143,750 $ 18,750 10 % $ 2.50 $ - $ 96,208 April 30, 2020 (1) 12 months $ 546,250 $ 546,250 $ 71,250 10 % $ 2.50 $ 47,500 $ 427,500 May 6, 2020 (1) 12 months $ 460,000 $ 460,000 $ 60,000 10 % $ 2.50 $ 40,000 $ 360,000 May 18, 2020 12 months $ 546,250 $ 221,250 $ 46,250 10 % $ 2.50 $ 35,500 $ 439,500 June 2, 2020 12 months $ 902,750 $ 652,750 $ 92,750 10 % $ 2.50 $ 58,900 $ 708,500 June 12, 2020 12 months $ 57,500 $ 57,500 $ 7,500 10 % $ 2.50 $ 5,000 $ 45,000 June 22, 2020 12 months $ 138,000 $ 138,000 $ 18,000 10 % $ 2.50 $ 12,000 $ 108,000 July 7, 2020 12 months $ 586,500 $ 586,500 $ 76,500 10 % $ 2.50 $ 51,000 $ 400,234 July 17, 2020 12 months $ 362,250 $ 362,250 $ 47,250 10 % $ 2.50 $ 31,500 $ 185,698 July 29, 2020 12 months $ 345,000 $ 345,000 $ 45,000 10 % $ 2.50 $ 30,000 $ 241,245 July 21, 2020 (5) 12 months $ 115,000 $ 115,000 $ 15,000 10 % $ 2.50 $ 10,000 $ 24,875 August 14, 2020 12 months $ 762,450 $ 462,450 $ 69,450 10 % $ 2.50 $ 66,300 $ 580,124 September 10, 2020 12 months $ 391,000 $ 391,000 $ 51,000 10 % $ 2.50 $ 34,000 $ 231,043 September 21, 2020 (5) 12 months $ 345,000 $ 345,000 $ 45,000 10 % $ 2.50 $ 30,000 $ 66,375 September 23, 2020 (5) 12 months $ 115,000 $ 115,000 $ 15,000 10 % $ 2.50 $ 10,000 $ 20,500 September 25, 2020 (5) 12 months $ 115,000 $ 115,000 $ 15,000 10 % $ 2.50 $ - $ 19,125 December 3, 2020 12 months $ 299,000 $ 299,000 $ 39,000 10 % $ 2.50 $ 26,000 $ 197,882 December 21, 2020 6 months $ 100,000 $ 100,000 $ 5,000 12 % $ 2.50 $ 29,964 $ 24,400 October 22, 2020 (5) 12 months $ 115,000 $ 115,000 $ 15,000 10 % $ 2.50 $ 10,000 $ 18,875 December 23, 2020 (5) 6 months $ 1,000,000 $ 1,000,000 $ 100,000 10 % $ 2.50 $ - $ 833,536 January 5, 2021 6 months $ 575,000 $ 575,000 $ 75,000 18 % $ 2.50 $ - $ - February 17, 2021 12 months $ 230,000 $ 230,000 $ 30,000 10 % $ 2.50 $ 20,000 $ 180,000 March 23, 2021 12 months $ 55,000 $ 55,000 $ 5,000 10 % $ 2.50 $ - $ 36,431 $ 12,077,587 $ 1,221,100 $ 907,214 $ 7,606,403 (1) The Note is past due. The Company and the lender are negotiating in good faith to extend the loan. (2) As of March 31, 2021 the Company and lender have agreed to the extension of the Standstill and Forbearance agreements (as described below). Loan is convertible at $2.50 as of March 31, 2021. (3) Interest was capitalized and added to outstanding principal. (4) During the year ended December 31,2020 the Company entered into Rate Modification Agreements with these lenders. In these agreements five lenders agreed to reduce their interest rate and were granted the right to convert loans using a variable conversion price if more than one other variable rate lender converted at a variable rate. (5) The Company has agreed to issue shares of its common stock to lenders if their notes are not repaid by a defined date. As of March 31, 2021 one lender holds approximately $7.5 million of the $12.1 million convertible notes outstanding. For the three months ended March 31, 2021, the Company recognized amortization expense related to the debt discounts indicated above of $2,124,788. The unamortized debt discounts as of March 31, 2021 related to the convertible debentures and other convertible notes amounted to $2,184,810. Standstill and Forbearance Agreements The Company has entered into Standstill and Forbearance Agreements with lenders who hold convertible notes with a total principal as of March 31, 2021 of $1.57 million. Pursuant to the Standstill and Forbearance Agreements, the lenders agreed to not convert any portion of their notes into shares of common stock at a variable rate until March 31, 2021 for convertible notes with a principal balance of $469 thousand and until April 16, 2021 for convertible notes with a principal balance of $1.1 million. For the three months ended March 31, 2021, the Company incurred fees of approximately $0.5 million in connection with these agreements. Convertible Loan Modifications and Extinguishments We refinanced certain convertible loans during the three months ended March 31, 2021 at substantially the same terms for extensions ranging over a period of three to six months. We amortized any remaining unamortized debt discount as of the modification date over the remaining, extended term of the new loans. We applied ASC 470 of modification accounting to the debt instruments which were modified during the quarter or those settled with new notes issued concurrently for the same amounts but different maturity dates. The terms such as the interest rate, prepayment penalties, and default rates will be the same over the new extensions. According to ASC 470, an exchange of debt instruments between or a modification of a debt instrument by a debtor and a creditor in a nontroubled debt situation is deemed to have been accomplished with debt instruments that are substantially different if the present value of the cash flows under the terms of the new debt instrument is at least 10 percent different from the present value of the remaining cash flows under the terms of the original instrument. If the terms of a debt instrument are changed or modified and the cash flow effect on a present value basis is less than 10 percent, the debt instruments are not considered to be substantially different and will be accounted for as modifications. The cash flows of new debt exceeded 10% of the remaining cash flows of the original debt on several loans. During the three months ended March 31, 2021 we recorded losses on extinguishment of liabilities of approximately $1.1 million by calculating the difference of the fair value of the new debt and the carrying value of the old debt. The following table provides a summary of the changes in convertible debt, net of unamortized discounts, during 2021: 2021 Balance at January 1, $ 7,545,670 Issuance of convertible debt, face value 860,000 Deferred financing cost (130,000 ) Beneficial conversion feature on convertible note (53,777 ) Debt discount from shares and warrants issued with debt (162,654 ) Payments (191,250 ) Conversion of debt into equity (100,000 ) Accretion of interest and amortization of debt discount to interest expense 2,124,788 Balance at March 31, 9,892,777 Less: current portion 9,892,777 Convertible debt, long-term portion $ – Other Notes On September 9, 2019 and February 28,2020 we received a total of $966,500 unsecured non-convertible loans from a private investor with a one-month term. During the year ended December 31, 2020, the Company received net proceeds of $463,500, issued 150,000 warrants to purchase common stock (five-year term and $3.50 exercise price) and repaid $275,000. The relative fair value of $185,660 of the warrants issued with the note was recorded as a debt discount to be amortized over the term of the notes. As of March 31, 2021 the Company owes $691,500 on these notes which are past due. The Company and the investor are negotiating in good faith to extend the loans. On October 1, 2019, the Company and the holder of the $170,000 non-convertible loan issued in May 2017 agreed to extend the term of the loan to December 31, 2019. The Company agreed to issue 1,200 shares of its common stock per month while the note remains outstanding. The note will continue to earn 10% annual interest. The loan is currently past due, and the Company and the investor are negotiating in good faith to extend the loan. On October 11, 2019 we received a non-convertible loan with a one-month term and a 2% interest charge for $25,000 from a private investor. The loan is past due, and the Company and the investor are negotiating in good faith to extend the loan. Merchant Agreements We have signed various Merchant Agreements which are secured by second position rights to all customer receipts until the loan has been repaid in full and subject to interest rates of 4% - 5.7% per month. As illustrated in the following table, under the terms of these agreements, we received the disclosed Purchase Price and agreed to repay the disclosed Purchase Amount, which is collected by the Merchant lenders at the disclosed Daily Payment Rate. The Company’s Chief Executive Officer is personally guaranteeing the performance of the merchant loans. The following table shows our Merchant Agreements as of March 31, 2021: Inception Date Purchase Price Purchased Outstanding Daily Rate Deferred Fees November 19, 2020 100,000 137,900 38,468 985.00 - February 4, 2021 125,000 165,000 102,084 1,032.00 - March 11, 2021 125,000 167,500 111,565 1,396.00 $ 2,500 March 26, 2021 240,000 330,960 236,257 2,364.00 - $ 590,000 $ 801,360 $ 488,374 $ 5,777.00 $ 2,500 The following table shows our Merchant Agreements as of December 31, 2020: Inception Date Purchase Price Purchased Outstanding Daily Rate Deferred Fees November 5, 2020 $ 200,000 $ 275,800 $ 163,955 1,724.00 $ - November 19, 2020 100,000 137,900 85,013 985.00 - $ 300,000 $ 413,700 $ 248,968 $ 2,709.00 $ - We have accounted for the Merchant Agreements as loans under ASC 860 because while we provided rights to current and future receipts, we still had control over the receipts. The difference between the Purchase Amount and the Purchase Price is imputed interest that is recorded as interest expense when paid each day. Related Party Notes In June 2018, we received a non-convertible loan of $15,000 from a private investor. The loan includes a one-year term and 15% guaranteed interest. This loan remains outstanding at March 31, 2021 and is currently past due. During the three months ended March 31, 2021, we received short-term non-convertible loans of $85,000 from related parties and repaid $70,000 of related party loans. These notes bear interest ranging from 5% to 15% and are due upon demand. Long term debt During the quarter ended March 31, 2021, the Company borrowed $367,038 through a COVID-19 program that was sponsored by the United States and administered by the Small Business Administration (the “SBA”). The most notable programs were the Payroll Protection Program (or “PPP”) and the Economic Injury Disaster Loan program (or “EIDL”). PPP loan has a 1% interest rate and a five-year term. During this period, the Company’s first PPP loan borrowed in 2020 ($367,039) was forgiven by the SBA. This gain was reported in losses on extinguishment of liabilities on the consolidated statements of operations. The Company’s EIDL loan, $150,000, accrues interest at 3.75% and requires monthly payments of $731 for principal and interest beginning in June 2021. The balance of the principal will be due in 30 years. In connection with the EIDL loan the Company entered into a security agreement with the SBA, whereby the Company granted the SBA a security interest in all of the Company’s right, title and interest in all of the Company’s assets.

Stockholders' Deficit

Stockholders' Deficit	3 Months Ended
	Mar. 31, 2021
Equity [Abstract]
Stockholders' Deficit	6) Stockholders’ Deficit Preferred Stock We are authorized to issue 1,000,000 shares of preferred stock with a par value of $0.01. Of the 1,000,000 shares of preferred stock: 1) 20,000 shares have been designated as Series A Junior Participating Preferred Stock (“ Junior A 2) 313,960 shares have been designated as Series A Convertible Preferred Stock (“ Series A 3) 279,256 shares have been designated as Series B Convertible Preferred Stock (“ Series B 4) 88,098 shares have been designated as Series C Convertible Preferred Stock (“ Series C 5) 850 shares have been designated as Series D Convertible Preferred Stock (“ Series D 6) 500 shares have been designated as Series E Convertible Preferred Stock (“Series E”) 7) 240,000 shares have been designated as Series G Convertible Preferred Stock (“ Series G 8) 10,000 shares have been designated as Series H Convertible Preferred Stock (“ Series H 9) 21 shares have been designated as Series H2 Convertible Preferred Stock (“ Series H2 10) 6,250 shares have been designated as Series J Convertible Preferred Stock (“ Series J 11) 15,000 shares have been designated as Series K Convertible Preferred Stock (“ Series K 12) 10,000 shares have been designated as Series AA Convertible Preferred Stock (“ Series AA As of March 31, 2021, there were no shares of Junior A, and Series A, B, C and E issued and outstanding. See our Annual Report on Form 10-K for the year ended December 31, 2020 for the pertinent disclosures of preferred stock. During the three months ended March 31, 2021, the Company entered into Securities Purchase Agreements with accredited investors pursuant to which the Company sold an aggregate of 40 shares of Series AA Convertible Preferred Stock, each preferred share convertible into 1,000 shares of the Company’s common stock, par value $0.01 per share, for an aggregate Purchase price of $100,000. We issued to the investors warrants to purchase an aggregate 40,000 shares of common stock with an exercise price of $3.50 per share. The Company did not incur any placement agent fees for this transaction. Stock Options and Warrants At the Company’s December 12, 2013 Special Meeting, the shareholders approved the 2013 Equity Incentive Plan (the “2013 Plan”) pursuant to which 3,000,000 shares of our common stock were reserved for issuance upon exercise of stock options or other equity awards. Under the 2013 Plan, we may award stock options, shares of common stock, and other equity interests in the Company to employees, officers, directors, consultants, and advisors, and to any other persons the Board of Directors deems appropriate. As of March 31, 2021, options to acquire 1,358,490 shares were outstanding under the Plan. As of March 31, 2021, total unrecognized compensation cost related to the unvested stock-based awards was $285,781, which is expected to be recognized over weighted average period of 1.68 years. The aggregate intrinsic value associated with the options outstanding and exercisable, and the aggregate intrinsic value associated with the warrants outstanding and exercisable as of March 31, 2021, based on the March 31, 2021 closing stock price of $2.10, was $1,298,874. The following table summarizes information concerning options and warrants outstanding and exercisable: Stock Options Warrants Weighted Weighted Average Average Shares price per share Shares price per share Shares Total Balance outstanding, December 31, 2020 1,355,901 $ 0.69 14,434,702 $ 3.50 15,790,603 15,302,830 Granted 24,000 2.17 561,200 3.50 585,200 Exercised (21,411 ) 0.69 - - (21,411 ) Expired - - (94,691 ) $ 3.50 (94,691 ) Balance outstanding, March 31, 2021 1,358,490 $ 0.69 14,901,211 $ 3.50 16,259,701 15,822,398 In the three months ended March 31, 2021 the Company issued 24,000 stock options to an employee ($49,135 fair value, $2.17 exercise price, three-year vesting term and ten-year expiration term). As of March 31, 2021, the 1,358,490 stock options outstanding have a $0.72 weighted average exercise price and 8.47 years weighted average remaining term. Of these options, 921,187 are currently exercisable. Common Stock and Warrant Issuances As profiled in the following table, for five loans we are obligated to issue common stock if not paid by defined dates. Loan Issuance Loan Shares Defined Defined Loan Date Principal Issuable Date Frequency Loan 1 July 21, 2020 $ 115,000 5,000 September 30, 2020 Monthly Loan 2 September 21, 2020 $ 345,000 12,500 November 16, 2020 Weekly Loan 3 September 23, 2020 $ 115,000 12,500 December 1, 2020 Weekly Loan 4 September 25, 2020 $ 115,000 12,500 December 1, 2020 Weekly Loan 5 October 22, 2020 $ 115,000 12,500 December 1, 2020 Weekly For our loan dated December 23, 2020, we are obligated to issue 100,000 warrants if the loan is not repaid before January 23, 2021 and an additional 10,000 shares of common stock and 100,000 warrants if the loan is not repaid before February 23, 2021. We are also obligated to issue 10,000 shares of common stock and 200,000 warrants if the loan is not repaid before March 23, 2021. During the quarter ended March 31,2021 the Company issued 400,000 warrants to this lender ($3.50 exercise price and five-year term) with a fair value of $600,298. The Company is also obligated to issue 10,000 shares of common stock to this lender every 31 days up to the loan’s maturity date on June 23, 2021. During the three months ended March 31, 2021, we issued 922,372 shares of common stock with a fair value of approximately $2.0 million to lenders for interest paid-in-kind, 112,400 shares with a fair value of $238,512 for services rendered, 47,200 shares with a fair value of $118,000 for conversions of debt principal and interest and 21,411 shares for stock option exercises at an exercise price of $0.69 per share. During this period, we also issued 521,200 warrants (five-year term at a $3.50 exercise price) to acquire common stock at a fair value of $0.8 million to lenders in conjunction with signing of new convertible loans and interest paid-in-kind. On various dates in the quarter ended March 31, 2020 the Company issued a total of 115,021 shares of restricted common stock at a fair value of approximately $213,415 to accredited investors and consultants. 66,500 of the shares with a fair value of $127,855 were issued to settle accrued liabilities to consultants, 38,521 of the shares with a fair value of $60,560 were issued for debt extension and interest payments and 10,000 shares with a fair value of $25,000 were issued for debt settlement. During the quarter ended March 31, 2020, the Company also issued 1,095,230 warrants to acquire common stock at a fair value of $1,205,010 in conjunction with the signing of new convertible loans and 307,500 warrants to acquire common stock at a fair value of $609,143 to lenders for debt extension.

Subsequent Events

Subsequent Events	3 Months Ended
	Mar. 31, 2021
Subsequent Events [Abstract]
Subsequent Events	7) Subsequent Events From April 1, 2021 through May 5, 2021 the Company received convertible loans for a total of $747,500. The Company issued 299,000 warrants (five-year life and $3.50 strike price) as fees paid to the lender. These loans have conversion prices of $2.50, carry interest rates of 10%, and terms of twelve months. In this time, the Company received $50,000 of new related party loans and entered into a Merchant Cash lender agreement (collecting $200,000 of which $27,580 was used to settle an existing merchant agreement dated November 19, 2020). Under the Merchant Cash agreement, the Company pays $1,970 each business day to the Merchant Cash lender until the lender has received cumulative payments of $275,800. The Company also partially repaid $300,000 of a loan dated December 23, 2020 and issued 331,370 shares of common stock (valued at $671,066) for interest-paid-in-kind.

Summary of Significant Accoun_2

Summary of Significant Accounting Policies (Policies)	3 Months Ended
	Mar. 31, 2021
Accounting Policies [Abstract]
Basis of Presentation	Basis of Presentation The unaudited interim financial statements of Pressure BioSciences, Inc. and its consolidated subsidiaries (collectively, the “Company”) included herein have been prepared by the Company in accordance with the instructions to Form 10-Q and the rules and regulations of the U.S. Securities and Exchange Commission. Under these rules and regulations, some information and footnote disclosures normally included in financial statements prepared under accounting principles generally accepted in the United States of America have been shortened or omitted. Management believes that all adjustments necessary for a fair statement of the financial position and the results of operations for the periods shown have been made. All adjustments are normal and recurring. These financial statements should be read together with the Company’s audited financial statements included in its Form 10-K for the fiscal year ended December 31, 2020.
Use of Estimates	Use of Estimates The Company’s consolidated financial statements and accompanying notes are prepared in accordance with accounting principles generally accepted in the United States of America, which require the use of estimates, judgements and assumptions that affect the reported amounts of assets and liabilities at the date of the financial statements and the reported amounts of revenues and expenses during the periods presented. Global concerns about the COVID-19 pandemic have adversely affected, and we expect will continue to adversely affect, our business, financial condition and results of operations including the estimates and assumptions made by management. Significant estimates and assumptions include valuations of share-based awards, investments in equity securities and intangible asset impairment. Actual results could differ from the estimates, and such differences may be material to the Company’s consolidated financial statements.
Principles of Consolidation	Principles of Consolidation The consolidated financial statements include the accounts of Pressure BioSciences, Inc., and its wholly owned subsidiary PBI BioSeq, Inc. All intercompany accounts and transactions have been eliminated in consolidation.
Revenue Recognition	Revenue Recognition We recognize revenue in accordance with FASB ASC 606, Revenue from Contracts with Customers, ASC 340-40, Other Assets and Deferred Costs—Contracts with Customers We identify a performance obligation as distinct if both the following criteria are true: the customer can benefit from the good or service either on its own or together with other resources that are readily available to the customer and the entity’s promise to transfer the good or service to the customer is separately identifiable from other promises in the contract. Determining the standalone selling price (“SSP”) and allocation of consideration from a contract to the individual performance obligations, and the appropriate timing of revenue recognition, is the result of significant qualitative and quantitative judgments. Management considers a variety of factors such as historical sales, usage rates, costs, and expected margin, which may vary over time depending upon the unique facts and circumstances related to each performance obligation in making these estimates. While changes in the allocation of the SSP between performance obligations will not affect the amount of total revenue recognized for a particular contract, any material changes could impact the timing of revenue recognition, which would have a material effect on our financial position and result of operations. This is because the contract consideration is allocated to each performance obligation, delivered or undelivered, at the inception of the contract based on the SSP of each distinct performance obligation. Taxes assessed by a governmental authority that are both imposed on and concurrent with a specific revenue-producing transaction, that are collected by the Company from a customer, are excluded from revenue. Shipping and handling costs associated with outbound freight after control over a product has transferred to a customer are accounted for as a fulfillment cost and are in included in cost of revenues as consistent with treatment in prior periods. Our current Barocycler® instruments require a basic level of instrumentation expertise to set-up for initial operation. To support a favorable first experience for our customers, upon customer request, and for an additional fee, we will send a highly trained technical representative to the customer site to install Barocyclers® that we sell, lease, or rent through our domestic sales force. The installation process includes uncrating and setting up the instrument, followed by introductory user training. Our sales arrangements do not provide our customers with a right of return. Any shipping costs billed to customers are recognized as revenue. The majority of our instrument and consumable contracts contain pricing that is based on the market price for the product at the time of delivery. Our obligations to deliver product volumes are typically satisfied and revenue is recognized when control of the product transfers to our customers. Concurrent with the transfer of control, we typically receive the right to payment for the shipped product and the customer has significant risks and rewards of ownership of the product. Payment terms require customers to pay shortly after delivery and do not contain significant financing components. Revenue from scientific services customers is recognized upon completion of each stage of service as defined in service agreements. We apply ASC 845, “Accounting for Non-Monetary Transactions”, to account for products and services sold through non-cash transactions based on the fair values of the products and services involved, where such values can be determined. Non-cash exchanges would require revenue to be recognized at recorded cost or carrying value of the assets or services sold if any of the following conditions apply: a) The fair value of the asset or service involved is not determinable. b) The transaction is an exchange of a product or property held for sale in the ordinary course of business for a product or property to be sold in the same line of business to facilitate sales to customers other than the parties to the exchange. c) The transaction lacks commercial substance. We recognize revenue for non-cash transactions at recorded cost or carrying value of the assets or services sold. We account for lease agreements of our instruments in accordance with ASC 842, Leases. We record revenue over the life of the lease term, and we record depreciation expense on a straight-line basis over the thirty-six-month estimated useful life of the Barocycler® instrument. The depreciation expense associated with assets under lease agreement is included in the “Cost of PCT products and services” line item in our accompanying consolidated statements of operations. Many of our lease and rental agreements allow the lessee to purchase the instrument at any point during the term of the agreement with partial or full credit for payments previously made. We pay all maintenance costs associated with the instrument during the term of the leases. Deferred revenue represents amounts received from service contracts for which the related revenues have not been recognized because one or more of the revenue recognition criteria have not been met. Revenue from service contracts is recorded ratably over the length of the contract. Disaggregation of revenue In the following table, revenue is disaggregated by primary geographical market, major product line, and timing of revenue recognition. In thousands of US dollars ($) Three Months Ended Primary geographical markets 2021 2020 North America $ 208 $ 143 Europe 84 54 Asia 268 57 $ 560 $ 254 Three Months Ended Major products/services lines 2021 2020 Hardware $ 377 $ 129 Consumables 102 56 Contract research services 6 10 Sample preparation accessories 29 25 Technical support/extended service contracts 24 19 Shipping and handling 19 9 Other 3 6 $ 560 $ 254 Three Months Ended Timing of revenue recognition 2021 2020 Products transferred at a point in time $ 538 $ 225 Services transferred over time 22 29 $ 560 $ 254 Contract balances In thousands of US dollars ($) March 31, 2021 December 31, 2020 Receivables, which are included in ‘Accounts Receivable’ $ 505 $ 131 Contract liabilities (deferred revenue) 54 67 Transaction price allocated to the remaining performance obligations. The following table includes estimated revenue expected to be recognized in the future related to performance obligations that are unsatisfied (or partially unsatisfied) at the end of the reporting period. In thousands of US dollars ($) 2021 2022 Total Extended warranty service $ 42 12 $ 54 All consideration from contracts with customers is included in the amounts presented above. Contract Costs The Company recognizes the incremental costs of obtaining contracts as an expense when incurred if the amortization period of the assets that the Company otherwise would have recognized is one year or less. These costs are included in selling, general, and administrative expenses. The costs to obtain a contract are recorded immediately in the period when the revenue is recognized either upon shipment or installation. The costs to obtain a service contract are considered immaterial when spread over the life of the contract so the Company records the costs immediately upon billing.
Concentrations	Concentrations Credit Risk Our financial instruments that potentially subject us to concentrations of credit risk consist primarily of cash, cash equivalents, and trade receivables. We have cash investment policies which, among other things, limit investments to investment-grade securities. We perform ongoing credit evaluations of our customers, and the risk with respect to trade receivables is further mitigated by the fact that many of our customers are government institutions, large pharmaceutical and biotechnology companies, and academic laboratories. The following table illustrates the level of concentration as a percentage of total revenues during the three months ended March 31, 2021 and 2020. For the Three Months Ended March 31, 2021 2020 Top Five Customers 88 % 68 % Federal Agencies 1 % 6 % The following table illustrates the level of concentration as a percentage of net accounts receivable balance as of March 31, 2021 and December 31, 2020. The Top Five Customers category may include federal agency receivable balances if applicable. March 31, 2021 December 31, 2020 Top Five Customers 94 % 89 % Federal Agencies 1 % 10 %
Product Supply	Product Supply We utilize a contract assembler for our Barocycler® 2320EXT. They provide us with precision manufacturing services that include management support services to meet our specific application and operational requirements. Among the services provided to us are: ● CNC Machining ● Contract Assembly & Kitting ● Component and Subassembly Design ● Inventory Management ● ISO certification At this time, we believe that outsourcing contract assembly of our Barocycler® 2320EXT is the most cost-effective method for us to obtain ISO Certified, CE and CSA Marked instruments. We currently manufacture and assemble the Barocycler®, HUB440, HUB880, the SHREDDER SG3, and most of our consumables at our South Easton, MA facility. We will regularly reassess the tradeoffs between in-house assembly versus the benefits of outsourced relationships for of the entire Barocycler® product line, and future instruments.
Investment in Equity Securities	Investment in Equity Securities As of March 31, 2021, we held 100,250 shares of common stock of Nexity Global SA, (a Polish publicly traded company). We account for this investment in accordance with ASC 320 “Investments — Debt and Equity Securities”. ASC 320 requires equity investments with readily determinable fair values to be measured at fair value with changes in fair value recognized in net income. As of March 31, 2021, our consolidated balance sheet reflected the fair value, determined on a recurring basis based on Level 1 inputs of our investment in Nexity, to be $409,098. We recorded $107,903 as an unrealized loss during the quarter ended March 31, 2021 for changes in market value.
Computation of Loss Per Share	Computation of Loss per Share Basic loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding. Diluted loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding plus additional common shares that would have been outstanding if dilutive potential common shares had been issued. For purposes of this calculation, convertible preferred stock, common stock dividends, and warrants and options to acquire common stock, are all considered common stock equivalents in periods in which they have a dilutive effect and are excluded from this calculation in periods in which these are anti-dilutive to our net loss. The following table illustrates our computation of loss per share for the three months ended March 31, 2021 and 2020: For the Three Months Ended March 31, 2021 2020 Numerator: Net loss attributable to common stockholders $ (7,038,686 ) $ (4,278,471 ) Denominator for basic and diluted loss per share: Weighted average common stock shares outstanding 4,865,826 2,648,039 Loss per common share – basic and diluted $ (1.45 ) $ (1.62 ) The following table presents securities that could potentially dilute basic loss per share in the future. For all periods presented, the potentially dilutive securities were not included in the computation of diluted loss per share because these securities would have been anti-dilutive to our net loss. The Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H and H2 Convertible Preferred Stock, Series J Convertible Preferred Stock, Series K Convertible Preferred Stock, and Series AA Convertible Preferred Stock are presented below as if they were converted into common shares according to the conversion terms. As of March 31, 2021 2020 Stock options 1,358,490 1,393,551 Convertible debt 4,757,701 3,125,633 Common stock warrants 14,901,211 11,295,764 Convertible preferred stock: Series D Convertible Preferred Stock 25,000 25,000 Series G Convertible Preferred Stock 26,857 26,857 Series H Convertible Preferred Stock 33,334 33,334 Series H2 Convertible Preferred Stock 70,000 70,000 Series J Convertible Preferred Stock 115,267 115,267 Series K Convertible Preferred Stock 229,334 229,334 Series AA Convertible Preferred Stock 8,083,000 7,939,000 29,600,194 24,253,740
Accounting for Stock-based Compensation Expense	Accounting for Stock-Based Compensation Expense We maintain equity compensation plans under which incentive stock options and non-qualified stock options are granted to employees, independent members of our Board of Directors and outside consultants. We recognize stock-based compensation expense over the requisite service period using the Black-Scholes formula to estimate the fair value of the stock options on the date of grant. Determining Fair Value of Stock Option Grants Valuation and Amortization Method - The fair value of each option award is estimated on the date of grant using the Black-Scholes pricing model based on certain assumptions. The estimated fair value of employee stock options is amortized to expense using the straight-line method over the vesting period. Expected Term - The Company uses the simplified calculation of expected life, as the Company does not currently have sufficient historical exercise data on which to base an estimate of expected term. Using this method, the expected term is determined using the average of the vesting period and the contractual life of the stock options granted. Expected Volatility - Expected volatility is based on the Company’s historical stock volatility data over the expected term of the award. Risk-Free Interest Rate - The Company bases the risk-free interest rate used in the Black-Scholes valuation method on the implied yield currently available on U.S. Treasury zero-coupon issues with an equivalent remaining term. Forfeitures - The Company records stock-based compensation expense only for those awards that are expected to vest. The Company estimated a forfeiture rate of 5% for awards granted based on historical experience and future expectations of options vesting. The Company used this historical rate as our assumption in calculating future stock-based compensation expense. The Company recognized stock-based compensation expense of $61,237 and $241,769 for the three months ended March 31, 2021 and 2020, respectively. The following table summarizes the effect of this stock-based compensation expense within each of the line items of our costs and expenses within our Consolidated Statements of Operations: For the Three Months Ended March 31, 2021 2020 Cost of sales $ 5,053 $ 7,956 Research and development 25,862 38,826 Selling and marketing 4,595 13,936 General and administrative 25,727 181,051 Total stock-based compensation expense $ 61,237 $ 241,769
Fair Value of Financial Instruments	Fair Value of Financial Instruments Due to their short maturities, the carrying amounts for cash and cash equivalents, accounts receivable, accounts payable, accrued expenses and debt approximate their fair value. Long-term liabilities include debt and deferred revenue with a carrying value that approximates fair value.
Fair Value Measurements	Fair Value Measurements The Company follows the guidance of FASB ASC Topic 820, “ Fair Value Measurements and Disclosures The Company generally defines fair value as the price that would be received to sell an asset or paid to transfer a liability in an orderly transaction between market participants at the measurement date (exit price). The Company uses a three-tier fair value hierarchy, which classifies the inputs used in measuring fair values. These tiers include: Level 1, defined as observable inputs such as quoted prices for identical instruments in active markets; Level 2, defined as inputs other than quoted prices in active markets that are either directly or indirectly observable; and Level 3, defined as unobservable inputs in which little or no market data exists, therefore requiring the Company to develop its own assumptions. A slight change in an unobservable input like volatility could have a significant impact on fair value measurement. Financial assets and liabilities are classified in their entirety based on the lowest level of input that is significant to the fair value measurement. The Company has determined that its financial assets are classified within Level 1 in the fair value hierarchy. The development of the unobservable inputs for Level 3 fair value measurements and fair value calculations are the responsibility of the Company’s management. The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of March 31, 2021: Fair value measurements at March 31, 2021 using: March 31, 2021 Quoted prices in active markets (Level 1) Significant other observable inputs (Level 2) Significant unobservable inputs (Level 3) Equity Securities $ 409,098 $ 409,098 - - Total Financial Assets $ 409,098 $ 409,098 $ - $ - The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of December 31, 2020: Fair value measurements at December 31, 2020 using: December 31, 2020 Quoted prices in active markets (Level 1) Significant other observable inputs (Level 2) Significant unobservable inputs (Level 3) Equity Securities 517,001 517,001 - - Total Financial Assets $ 517,001 $ 517,001 $ - $ -

Summary of Significant Accoun_3

Summary of Significant Accounting Policies (Tables)	3 Months Ended
	Mar. 31, 2021
Accounting Policies [Abstract]
Schedule of Disaggregation of Revenue	In the following table, revenue is disaggregated by primary geographical market, major product line, and timing of revenue recognition. In thousands of US dollars ($) Three Months Ended Primary geographical markets 2021 2020 North America $ 208 $ 143 Europe 84 54 Asia 268 57 $ 560 $ 254 Three Months Ended Major products/services lines 2021 2020 Hardware $ 377 $ 129 Consumables 102 56 Contract research services 6 10 Sample preparation accessories 29 25 Technical support/extended service contracts 24 19 Shipping and handling 19 9 Other 3 6 $ 560 $ 254 Three Months Ended Timing of revenue recognition 2021 2020 Products transferred at a point in time $ 538 $ 225 Services transferred over time 22 29 $ 560 $ 254
Schedule of Contract Balances	Contract balances In thousands of US dollars ($) March 31, 2021 December 31, 2020 Receivables, which are included in ‘Accounts Receivable’ $ 505 $ 131 Contract liabilities (deferred revenue) 54 67
Schedule of Future Related to Performance Obligations	The following table includes estimated revenue expected to be recognized in the future related to performance obligations that are unsatisfied (or partially unsatisfied) at the end of the reporting period. In thousands of US dollars ($) 2021 2022 Total Extended warranty service $ 42 12 $ 54
Schedule of Customer Concentration Risk Percentage	The following table illustrates the level of concentration as a percentage of total revenues during the three months ended March 31, 2021 and 2020. For the Three Months Ended March 31, 2021 2020 Top Five Customers 88 % 68 % Federal Agencies 1 % 6 % The following table illustrates the level of concentration as a percentage of net accounts receivable balance as of March 31, 2021 and December 31, 2020. The Top Five Customers category may include federal agency receivable balances if applicable. March 31, 2021 December 31, 2020 Top Five Customers 94 % 89 % Federal Agencies 1 % 10 %
Schedule of Computation of Loss Per Share	The following table illustrates our computation of loss per share for the three months ended March 31, 2021 and 2020: For the Three Months Ended March 31, 2021 2020 Numerator: Net loss attributable to common stockholders $ (7,038,686 ) $ (4,278,471 ) Denominator for basic and diluted loss per share: Weighted average common stock shares outstanding 4,865,826 2,648,039 Loss per common share – basic and diluted $ (1.45 ) $ (1.62 )
Schedule of Anti-dilutive Securities Excluded from Computation of Earnings Per Share	The following table presents securities that could potentially dilute basic loss per share in the future. For all periods presented, the potentially dilutive securities were not included in the computation of diluted loss per share because these securities would have been anti-dilutive to our net loss. The Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H and H2 Convertible Preferred Stock, Series J Convertible Preferred Stock, Series K Convertible Preferred Stock, and Series AA Convertible Preferred Stock are presented below as if they were converted into common shares according to the conversion terms. As of March 31, 2021 2020 Stock options 1,358,490 1,393,551 Convertible debt 4,757,701 3,125,633 Common stock warrants 14,901,211 11,295,764 Convertible preferred stock: Series D Convertible Preferred Stock 25,000 25,000 Series G Convertible Preferred Stock 26,857 26,857 Series H Convertible Preferred Stock 33,334 33,334 Series H2 Convertible Preferred Stock 70,000 70,000 Series J Convertible Preferred Stock 115,267 115,267 Series K Convertible Preferred Stock 229,334 229,334 Series AA Convertible Preferred Stock 8,083,000 7,939,000 29,600,194 24,253,740
Schedule of Stock Based Compensation Expense	The following table summarizes the effect of this stock-based compensation expense within each of the line items of our costs and expenses within our Consolidated Statements of Operations: For the Three Months Ended March 31, 2021 2020 Cost of sales $ 5,053 $ 7,956 Research and development 25,862 38,826 Selling and marketing 4,595 13,936 General and administrative 25,727 181,051 Total stock-based compensation expense $ 61,237 $ 241,769
Schedule of Assets and Liabilities Measured at Fair Value on Recurring Basis	The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of March 31, 2021: Fair value measurements at March 31, 2021 using: March 31, 2021 Quoted prices in active markets (Level 1) Significant other observable inputs (Level 2) Significant unobservable inputs (Level 3) Equity Securities $ 409,098 $ 409,098 - - Total Financial Assets $ 409,098 $ 409,098 $ - $ - The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of December 31, 2020: Fair value measurements at December 31, 2020 using: December 31, 2020 Quoted prices in active markets (Level 1) Significant other observable inputs (Level 2) Significant unobservable inputs (Level 3) Equity Securities 517,001 517,001 - - Total Financial Assets $ 517,001 $ 517,001 $ - $ -

Commitments and Contingencies (

Commitments and Contingencies (Tables)	3 Months Ended
	Mar. 31, 2021
Commitments and Contingencies Disclosure [Abstract]
Schedule of Future Minimum Rental Payments Required Under Operating Leases	Following is a schedule by years of future minimum rental payments required under operating leases with initial or remaining non-cancelable lease terms as of March 31, 2021: 2021 $ 128,087 2022 87,383 2023 87,383 Total minimum payments required $ 302,853

Convertible Debt and Other De_2

Convertible Debt and Other Debt (Tables)	3 Months Ended
	Mar. 31, 2021
Debt Disclosure [Abstract]
Schedule of Convertible Debts and Outstanding Balances	The specific terms of the convertible notes and outstanding balances as of March 31, 2021 are listed in the tables below. Inception Date Term Loan Amount Outstanding balance with OID Original Issue Discount (OID) Interest Rate Conversion Price Deferred Finance Fees Discount for conversion feature and warrants/shares May 17, 2018 (2) 12 months $ 380,000 $ 166,703 $ 15,200 8 % $ 2.50 $ 15,200 $ 332,407 October 19, 2018 (1) 6 months $ 100,000 $ 100,000 $ - 5 % $ 7.50 $ - $ - November 13, 2018 (1) (3) (4) 6 months $ 200,000 $ 220,000 $ - 5 % $ 2.50 $ - $ 168,634 January 3, 2019 (1)(4) 6 months $ 50,000 $ 50,000 $ 2,500 24 % $ 7.50 $ 2,500 $ - February 21, 2019 (2) 12 months $ 215,000 $ 215,000 $ - 4 % $ 2.50 $ 15,000 $ 107,709 March 18, 2019 (1) 6 months $ 100,000 $ 100,000 $ - 4 % $ 7.50 $ - $ 10,762 June 4, 2019 (2) 9 months $ 500,000 $ 302,484 $ - 8 % $ 2.50 $ 40,500 $ 70,631 June 19, 2019 (2) 12 months $ 105,000 $ 105,000 $ - 4 % $ 2.50 $ 5,000 $ 2,646 June 7, 2019 (1) (4) 6 months $ 125,000 $ 25,000 $ - 5 % $ 7.50 $ - $ 18,254 July 1, 2019 (2) 12 months $ 107,500 $ 107,500 $ - 4 % $ 2.50 $ 7,500 $ 85,791 July 19, 2019 (2) 12 months $ 115,000 $ 115,000 $ - 4 % $ 2.50 $ 5,750 $ 15,460 July 19, 2019 (2) 12 months $ 130,000 $ 130,000 $ - 6 % $ 2.50 $ 6,500 $ - September 27,2019 (2) 12 months $ 78,750 $ 78,750 $ - 4 % $ 2.50 $ 3,750 $ 13,759 October 24, 2019 (2) 12 months $ 78,750 $ 78,750 $ - 4 % $ 2.50 $ 3,750 $ - November 1, 2019 (2) 12 months $ 270,000 $ 270,000 $ - 6 % $ 2.50 $ 13,500 $ - November 15, 2019 (1) 12 months $ 385,000 $ 320,000 $ 35,000 10 % $ 2.50 $ 35,000 $ 90,917 January 2, 2020 (1) 12 months $ 330,000 $ 330,000 $ 30,000 10 % $ 2.50 $ 30,000 $ 91,606 January 24, 2020 (1) 12 months $ 247,500 $ 247,500 $ 22,500 10 % $ 2.50 $ 22,500 $ 89,707 January 29, 2020 (1) 12 months $ 363,000 $ 363,000 $ 33,000 10 % $ 2.50 $ 33,000 $ 297,000 February 12, 2020 (1) 12 months $ 275,000 $ 275,000 $ 25,000 10 % $ 2.50 $ 25,000 $ 225,000 February 19, 2020 (1) 12 months $ 165,000 $ 165,000 $ 15,000 10 % $ 2.50 $ 15,000 $ 135,000 March 11, 2020 (1) 12 months $ 330,000 $ 330,000 $ 30,000 10 % $ 2.50 $ 30,000 $ 232,810 March 13, 2020 (1) 12 months $ 165,000 $ 165,000 $ 15,000 10 % $ 2.50 $ 15,000 $ 60,705 March 26, 2020 (1) 12 months $ 111,100 $ 111,100 $ 10,100 10 % $ 2.50 $ 10,100 $ 90,900 April 8, 2020 (1) 12 months $ 276,100 $ 276,100 $ 25,100 10 % $ 2.50 $ 25,000 $ 221,654 April 17, 2020 (1) 12 months $ 143,750 $ 143,750 $ 18,750 10 % $ 2.50 $ - $ 96,208 April 30, 2020 (1) 12 months $ 546,250 $ 546,250 $ 71,250 10 % $ 2.50 $ 47,500 $ 427,500 May 6, 2020 (1) 12 months $ 460,000 $ 460,000 $ 60,000 10 % $ 2.50 $ 40,000 $ 360,000 May 18, 2020 12 months $ 546,250 $ 221,250 $ 46,250 10 % $ 2.50 $ 35,500 $ 439,500 June 2, 2020 12 months $ 902,750 $ 652,750 $ 92,750 10 % $ 2.50 $ 58,900 $ 708,500 June 12, 2020 12 months $ 57,500 $ 57,500 $ 7,500 10 % $ 2.50 $ 5,000 $ 45,000 June 22, 2020 12 months $ 138,000 $ 138,000 $ 18,000 10 % $ 2.50 $ 12,000 $ 108,000 July 7, 2020 12 months $ 586,500 $ 586,500 $ 76,500 10 % $ 2.50 $ 51,000 $ 400,234 July 17, 2020 12 months $ 362,250 $ 362,250 $ 47,250 10 % $ 2.50 $ 31,500 $ 185,698 July 29, 2020 12 months $ 345,000 $ 345,000 $ 45,000 10 % $ 2.50 $ 30,000 $ 241,245 July 21, 2020 (5) 12 months $ 115,000 $ 115,000 $ 15,000 10 % $ 2.50 $ 10,000 $ 24,875 August 14, 2020 12 months $ 762,450 $ 462,450 $ 69,450 10 % $ 2.50 $ 66,300 $ 580,124 September 10, 2020 12 months $ 391,000 $ 391,000 $ 51,000 10 % $ 2.50 $ 34,000 $ 231,043 September 21, 2020 (5) 12 months $ 345,000 $ 345,000 $ 45,000 10 % $ 2.50 $ 30,000 $ 66,375 September 23, 2020 (5) 12 months $ 115,000 $ 115,000 $ 15,000 10 % $ 2.50 $ 10,000 $ 20,500 September 25, 2020 (5) 12 months $ 115,000 $ 115,000 $ 15,000 10 % $ 2.50 $ - $ 19,125 December 3, 2020 12 months $ 299,000 $ 299,000 $ 39,000 10 % $ 2.50 $ 26,000 $ 197,882 December 21, 2020 6 months $ 100,000 $ 100,000 $ 5,000 12 % $ 2.50 $ 29,964 $ 24,400 October 22, 2020 (5) 12 months $ 115,000 $ 115,000 $ 15,000 10 % $ 2.50 $ 10,000 $ 18,875 December 23, 2020 (5) 6 months $ 1,000,000 $ 1,000,000 $ 100,000 10 % $ 2.50 $ - $ 833,536 January 5, 2021 6 months $ 575,000 $ 575,000 $ 75,000 18 % $ 2.50 $ - $ - February 17, 2021 12 months $ 230,000 $ 230,000 $ 30,000 10 % $ 2.50 $ 20,000 $ 180,000 March 23, 2021 12 months $ 55,000 $ 55,000 $ 5,000 10 % $ 2.50 $ - $ 36,431 $ 12,077,587 $ 1,221,100 $ 907,214 $ 7,606,403 (1) The Note is past due. The Company and the lender are negotiating in good faith to extend the loan. (2) As of March 31, 2021 the Company and lender have agreed to the extension of the Standstill and Forbearance agreements (as described below). Loan is convertible at $2.50 as of March 31, 2021. (3) Interest was capitalized and added to outstanding principal. (4) During the year ended December 31,2020 the Company entered into Rate Modification Agreements with these lenders. In these agreements five lenders agreed to reduce their interest rate and were granted the right to convert loans using a variable conversion price if more than one other variable rate lender converted at a variable rate. (5) The Company has agreed to issue shares of its common stock to lenders if their notes are not repaid by a defined date.
Summary of Changes in Convertible Debt and Revolving Note Payable, Net of Unamortized Discounts	The following table provides a summary of the changes in convertible debt, net of unamortized discounts, during 2021: 2021 Balance at January 1, $ 7,545,670 Issuance of convertible debt, face value 860,000 Deferred financing cost (130,000 ) Beneficial conversion feature on convertible note (53,777 ) Debt discount from shares and warrants issued with debt (162,654 ) Payments (191,250 ) Conversion of debt into equity (100,000 ) Accretion of interest and amortization of debt discount to interest expense 2,124,788 Balance at March 31, 9,892,777 Less: current portion 9,892,777 Convertible debt, long-term portion $ –
Schedule of Merchant Agreements	The following table shows our Merchant Agreements as of March 31, 2021: Inception Date Purchase Price Purchased Outstanding Daily Rate Deferred Fees November 19, 2020 100,000 137,900 38,468 985.00 - February 4, 2021 125,000 165,000 102,084 1,032.00 - March 11, 2021 125,000 167,500 111,565 1,396.00 $ 2,500 March 26, 2021 240,000 330,960 236,257 2,364.00 - $ 590,000 $ 801,360 $ 488,374 $ 5,777.00 $ 2,500 The following table shows our Merchant Agreements as of December 31, 2020: Inception Date Purchase Price Purchased Outstanding Daily Rate Deferred Fees November 5, 2020 $ 200,000 $ 275,800 $ 163,955 1,724.00 $ - November 19, 2020 100,000 137,900 85,013 985.00 - $ 300,000 $ 413,700 $ 248,968 $ 2,709.00 $ -

Stockholders' Deficit (Tables)

Stockholders' Deficit (Tables)	3 Months Ended
	Mar. 31, 2021
Equity [Abstract]
Schedule of Concerning Options and Warrants Outstanding and Exercisable	The following table summarizes information concerning options and warrants outstanding and exercisable: Stock Options Warrants Weighted Weighted Average Average Shares price per share Shares price per share Shares Total Balance outstanding, December 31, 2020 1,355,901 $ 0.69 14,434,702 $ 3.50 15,790,603 15,302,830 Granted 24,000 2.17 561,200 3.50 585,200 Exercised (21,411 ) 0.69 - - (21,411 ) Expired - - (94,691 ) $ 3.50 (94,691 ) Balance outstanding, March 31, 2021 1,358,490 $ 0.69 14,901,211 $ 3.50 16,259,701 15,822,398
Schedule of Loans Obligated to Issue Shares	As profiled in the following table, for five loans we are obligated to issue common stock if not paid by defined dates. Loan Issuance Loan Shares Defined Defined Loan Date Principal Issuable Date Frequency Loan 1 July 21, 2020 $ 115,000 5,000 September 30, 2020 Monthly Loan 2 September 21, 2020 $ 345,000 12,500 November 16, 2020 Weekly Loan 3 September 23, 2020 $ 115,000 12,500 December 1, 2020 Weekly Loan 4 September 25, 2020 $ 115,000 12,500 December 1, 2020 Weekly Loan 5 October 22, 2020 $ 115,000 12,500 December 1, 2020 Weekly

Summary of Significant Accoun_4

Summary of Significant Accounting Policies (Details Narrative) - USD ($)	1 Months Ended	3 Months Ended
	Mar. 31, 2020	Mar. 31, 2021	Mar. 31, 2020
Forfeiture rate		5.00%
Stock-based compensation expense		$ 61,237	$ 241,769
Nexity Global SA [Member]
Sale of stock number of shares received	100,250
Fair value of investment	$ 409,098		$ 409,098
Unrealized loss on changes in market value		$ 107,903

Summary of Significant Accoun_5

Summary of Significant Accounting Policies - Schedule of Disaggregation of Revenue (Details) - USD ($)	3 Months Ended
	Mar. 31, 2021	Mar. 31, 2020
Revenue	$ 559,874	$ 253,873
Hardware [Member]
Revenue	377,000	129,000
Consumables [Member]
Revenue	102,000	56,000
Contract Research Services [Member]
Revenue	6,000	10,000
Sample Preparation Accessories [Member]
Revenue	29,000	25,000
Technical Support/Extended Service Contracts [Member]
Revenue	24,000	19,000
Shipping and Handling [Member]
Revenue	19,000	9,000
Other [Member]
Revenue	3,000	6,000
Products Transferred at a Point in Time [Member]
Revenue	538,000	225,000
Services Transferred Over Time [Member]
Revenue	22,000	29,000
North America [Member]
Revenue	208,000	143,000
Europe [Member]
Revenue	84,000	54,000
Asia [Member]
Revenue	$ 268,000	$ 57,000

Summary of Significant Accoun_6

Summary of Significant Accounting Policies - Schedule of Contract Balances (Details) - USD ($)	Mar. 31, 2021	Dec. 31, 2020
Accounting Policies [Abstract]
Receivables, which are included in 'Accounts Receivable'	$ 505,000	$ 131,000
Contract liabilities (deferred revenue)	$ 54,000	$ 67,000

Summary of Significant Accoun_7

Summary of Significant Accounting Policies - Schedule of Future Related to Performance Obligations (Details)	Mar. 31, 2021USD ($)
Extended warranty service	$ 54,000
2021 [Member]
Extended warranty service	42,000
2022 [Member]
Extended warranty service	$ 12,000

Summary of Significant Accoun_8

Summary of Significant Accounting Policies - Schedule of Customer Concentration Risk Percentage (Details)	3 Months Ended		12 Months Ended
	Mar. 31, 2021	Mar. 31, 2020	Dec. 31, 2020
Top Five Customers [Member] | Revenue [Member]
Concentration credit risk percentage	88.00%	68.00%
Top Five Customers [Member] | Accounts Receivable [Member]
Concentration credit risk percentage	94.00%		89.00%
Federal Agencies [Member] | Revenue [Member]
Concentration credit risk percentage	1.00%	6.00%
Federal Agencies [Member] | Accounts Receivable [Member]
Concentration credit risk percentage	1.00%		10.00%

Summary of Significant Accoun_9

Summary of Significant Accounting Policies - Schedule of Computation of Loss Per Share (Details) - USD ($)	3 Months Ended
	Mar. 31, 2021	Mar. 31, 2020
Accounting Policies [Abstract]
Net loss attributable to common stockholders	$ (7,038,686)	$ (4,278,471)
Weighted average common stock shares outstanding	4,865,826	2,648,039
Loss per common share - basic and diluted	$ (1.45)	$ (1.62)

Summary of Significant Accou_10

Summary of Significant Accounting Policies - Schedule of Anti-dilutive Securities Excluded from Computation of Earnings Per Share (Details) - shares	3 Months Ended
	Mar. 31, 2021	Mar. 31, 2020
Total potentially dilutive shares	29,600,194	24,253,740
Stock Options [Member]
Total potentially dilutive shares	1,358,490	1,393,551
Convertible Debt [Member]
Total potentially dilutive shares	4,757,701	3,125,633
Common Stock Warrants [Member]
Total potentially dilutive shares	14,901,211	11,295,764
Series D Convertible Preferred Stock [Member]
Total potentially dilutive shares	25,000	25,000
Series G Convertible Preferred Stock [Member]
Total potentially dilutive shares	26,857	26,857
Series H Convertible Preferred Stock [Member]
Total potentially dilutive shares	33,334	33,334
Series H2 Convertible Preferred Stock [Member]
Total potentially dilutive shares	70,000	70,000
Series J Convertible Preferred Stock [Member]
Total potentially dilutive shares	115,267	115,267
Series K Convertible Preferred Stock [Member]
Total potentially dilutive shares	229,334	229,334
Series AA Convertible Preferred Stock [Member]
Total potentially dilutive shares	8,083,000	7,939,000

Summary of Significant Accou_11

Summary of Significant Accounting Policies - Schedule of Stock Based Compensation Expense (Details) - USD ($)	3 Months Ended
	Mar. 31, 2021	Mar. 31, 2020
Total stock-based compensation expense	$ 61,237	$ 241,769
Cost of Sales [Member]
Total stock-based compensation expense	5,053	7,956
Research and Development Expense [Member]
Total stock-based compensation expense	25,862	38,826
Selling and Marketing Expense [Member]
Total stock-based compensation expense	4,595	13,936
General and Administrative Expense [Member]
Total stock-based compensation expense	$ 25,727	$ 181,051

Summary of Significant Accou_12

Summary of Significant Accounting Policies - Schedule of Assets and Liabilities Measured at Fair Value on Recurring Basis (Details) - USD ($)	Mar. 31, 2021	Dec. 31, 2020
Total Financial Assets	$ 409,098	$ 517,001
Quoted Prices in Active Markets (Level 1) [Member]
Total Financial Assets	409,098	517,001
Significant Other Observable Inputs (Level 2) [Member]
Total Financial Assets
Significant Unobservable Inputs (Level 3) [Member]
Total Financial Assets
Equity Securities [Member]
Total Financial Assets	409,098	517,001
Equity Securities [Member] | Quoted Prices in Active Markets (Level 1) [Member]
Total Financial Assets	409,098	517,001
Equity Securities [Member] | Significant Other Observable Inputs (Level 2) [Member]
Total Financial Assets
Equity Securities [Member] | Significant Unobservable Inputs (Level 3) [Member]
Total Financial Assets

Commitments and Contingencies_2

Commitments and Contingencies (Details Narrative) - USD ($)	3 Months Ended	12 Months Ended
	Mar. 31, 2021	Dec. 31, 2020
Lease monthly payments	$ 7,282
Lease expire date	Dec. 30, 2023
Lease expiration term	The lease shall be automatically extended for additional three years unless either party terminates at least six months prior to the expiration of the current lease term.
Estimated borrowing rate	12.00%
Operating liability	$ 221,432
Corporate Office [Member]
Lease monthly payments		$ 6,950
Lease expire date		Dec. 31, 2021

Commitments and Contingencies -

Commitments and Contingencies - Schedule of Future Minimum Rental Payments Required Under Operating Leases (Details)	Mar. 31, 2021USD ($)
Commitments and Contingencies Disclosure [Abstract]
2021	$ 128,087
2022	87,383
2023	87,383
Total minimum payments required	$ 302,853

Convertible Debt and Other De_3

Convertible Debt and Other Debt (Details Narrative) - USD ($)	Dec. 23, 2020	Feb. 28, 2020	Oct. 11, 2019	Oct. 01, 2019	Sep. 09, 2019	May 05, 2021	Mar. 31, 2021	Mar. 31, 2020	Dec. 31, 2020	Apr. 16, 2021	Jun. 30, 2018
Proceeds from convertible notes							$ 730,000	$ 1,865,500
Convertible, beneficial conversion feature							53,777
Convertible notes outstanding							9,892,777		$ 7,545,670
Unamortized debt discount							2,184,810		3,948,167
Issuance of convertible debt, face value							860,000
Incurred fees							2,500
Gains (losses) on extinguishment of liabilities							$ (725,159)	(1,136,367)
Warrants term							5 years
Warrant exercise price per share							$ 3.50
Repayments of related party debt							$ 70,000
Debt monthly payment amount							$ 5,777		2,709
Small Business Administration [Member]
Convertible debentures term							5 years
Interest rate							1.00%
Issuance of convertible debt, face value							$ 367,038
Debt instrument forgiven amount							$ 367,039
Subsequent Event [Member]
Interest rate						10.00%
Debt conversion price per share						$ 2.50
Proceeds from loan						$ 747,500
Warrants term						5 years
Warrant exercise price per share						$ 3.50
Standstill and Forbearance Agreements [Member]
Debt conversion price per share							$ 2.50
Convertible notes outstanding							$ 469,000
Incurred fees							$ 500,000
Standstill and Forbearance Agreements [Member] | Subsequent Event [Member]
Convertible notes outstanding										$ 1,100,000
Merchant Agreements [Member]
Repayments of notes	$ 300,000
Economic Injury Disaster Loan Program [Member]
Convertible debentures term							30 years
Interest rate							3.75%
Issuance of convertible debt, face value							$ 150,000
Debt monthly payment amount							731
One Lender [Member]
Convertible notes outstanding							$ 7,500,000
Lenders [Member]
Warrants term							5 years
Warrant exercise price per share							$ 3.50
Lenders [Member] | Standstill and Forbearance Agreements [Member]
Issuance of convertible debt, face value							$ 1,570,000
Convertible Common Stock [Member]
Debt conversion price per share							$ 2.50
Fair value of converible notes							$ 162,654
Minimum [Member] | Merchant Agreements [Member]
Interest rate							4.00%
Maximum [Member] | Merchant Agreements [Member]
Interest rate							5.70%
Convertible Notes [Member]
Proceeds from convertible notes							$ 860,000
Convertible, beneficial conversion feature							53,777
Convertible notes outstanding									12,100,000
Amortization of debt discount							2,124,788
Unamortized debt discount							$ 2,184,810
Convertible Notes [Member] | Minimum [Member]
Convertible debentures term							6 months
Interest rate							10.00%
Convertible Notes [Member] | Maximum [Member]
Convertible debentures term							12 months
Interest rate							18.00%
New Loan [Member]
Interest rate							10.00%
Debt interest, description							According to ASC 470, an exchange of debt instruments between or a modification of a debt instrument by a debtor and a creditor in a nontroubled debt situation is deemed to have been accomplished with debt instruments that are substantially different if the present value of the cash flows under the terms of the new debt instrument is at least 10 percent different from the present value of the remaining cash flows under the terms of the original instrument. If the terms of a debt instrument are changed or modified and the cash flow effect on a present value basis is less than 10 percent, the debt instruments are not considered to be substantially different and will be accounted for as modifications.
Gains (losses) on extinguishment of liabilities							$ 1,100,000
Non-Convertible Loans [Member] | Private Investor [Member]
Convertible debentures term			1 month
Interest rate			2.00%
Issuance of convertible debt, face value							691,500
Proceeds from loan		$ 966,500	$ 25,000		$ 966,500				$ 463,500
Non-Convertible Loans [Member] | Private Investor [Member] | Warrants [Member]
Warrant to purchase shares of common stock									150,000
Warrants term									5 years
Warrant exercise price per share									$ 3.50
Repayments of notes									$ 275,000
Fair value of the warrants									$ 185,660
Non-Convertible Loans [Member] | Holder [Member]
Interest rate				10.00%
Proceeds from loan				$ 170,000
Number of common stock shares issued, shares				1,200
Non-Convertible Loan [Member] | Private Investor [Member]
Interest rate											15.00%
Loan amount											$ 15,000
Short-term Non-Convertible Loan [Member]
Proceeds from short term loan							85,000
Repayments of related party debt							$ 70,000
Short-term Non-Convertible Loan [Member] | Minimum [Member]
Interest rate							15.00%
Short-term Non-Convertible Loan [Member] | Maximum [Member]
Interest rate							5.00%

Convertible Debt and Other De_4

Convertible Debt and Other Debt - Schedule of Convertible Debts and Outstanding Balances (Details) - USD ($)		3 Months Ended
		Mar. 31, 2021	Dec. 31, 2020
Loan Amount		$ 860,000
Outstanding balance with OID		9,892,777	$ 7,545,670
Original Issue Discount (OID)		100,000
Deferred Finance Fees		2,500
Discount for conversion feature and warrants/shares		53,777
Convertible Notes [Member]
Outstanding balance with OID		12,077,587
Original Issue Discount (OID)		1,221,100
Deferred Finance Fees		907,214
Discount for conversion feature and warrants/shares		$ 7,606,403
Convertible Notes [Member] | Convertible Debt One [Member]
Inception Date	[1]	May 17, 2018
Term		12 months
Loan Amount		$ 380,000
Outstanding balance with OID		166,703
Original Issue Discount (OID)		$ 15,200
Interest Rate		8.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 15,200
Discount for conversion feature and warrants/shares		$ 332,407
Convertible Notes [Member] | Convertible Debt Two [Member]
Inception Date	[2]	October 19, 2018
Term		6 months
Loan Amount		$ 100,000
Outstanding balance with OID		100,000
Original Issue Discount (OID)
Interest Rate		5.00%
Conversion Price		$ 7.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares
Convertible Notes [Member] | Convertible Debt Three [Member]
Inception Date	[2],[3],[4]	November 13, 2018
Term		6 months
Loan Amount		$ 200,000
Outstanding balance with OID		220,000
Original Issue Discount (OID)
Interest Rate		5.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 168,634
Convertible Notes [Member] | Convertible Debt Four [Member]
Inception Date	[2],[3]	January 3, 2019
Term		6 months
Loan Amount		$ 50,000
Outstanding balance with OID		50,000
Original Issue Discount (OID)		$ 2,500
Interest Rate		24.00%
Conversion Price		$ 7.50
Deferred Finance Fees		$ 2,500
Discount for conversion feature and warrants/shares
Convertible Notes [Member] | Convertible Debt Five [Member]
Inception Date	[1]	February 21, 2019
Term		12 months
Loan Amount		$ 215,000
Outstanding balance with OID		215,000
Original Issue Discount (OID)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 15,000
Discount for conversion feature and warrants/shares		$ 107,709
Convertible Notes [Member] | Convertible Debt Six [Member]
Inception Date	[2]	March 18, 2019
Term		6 months
Loan Amount		$ 100,000
Outstanding balance with OID		100,000
Original Issue Discount (OID)
Interest Rate		4.00%
Conversion Price		$ 7.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 10,762
Convertible Notes [Member] | Convertible Debt Seven [Member]
Inception Date	[1]	June 4, 2019
Term		9 months
Loan Amount		$ 500,000
Outstanding balance with OID		302,484
Original Issue Discount (OID)
Interest Rate		8.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 40,500
Discount for conversion feature and warrants/shares		$ 70,631
Convertible Notes [Member] | Convertible Debt Eight [Member]
Inception Date	[1]	June 19, 2019
Term		12 months
Loan Amount		$ 105,000
Outstanding balance with OID		105,000
Original Issue Discount (OID)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 5,000
Discount for conversion feature and warrants/shares		$ 2,646
Convertible Notes [Member] | Convertible Debt Nine [Member]
Inception Date	[2],[3]	June 7, 2019
Term		6 months
Loan Amount		$ 125,000
Outstanding balance with OID		25,000
Original Issue Discount (OID)
Interest Rate		5.00%
Conversion Price		$ 7.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 18,254
Convertible Notes [Member] | Convertible Debt Ten [Member]
Inception Date	[1]	July 1, 2019
Term		12 months
Loan Amount		$ 107,500
Outstanding balance with OID		107,500
Original Issue Discount (OID)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 7,500
Discount for conversion feature and warrants/shares		$ 85,791
Convertible Notes [Member] | Convertible Debt Eleven [Member]
Inception Date	[1]	July 19, 2019
Term		12 months
Loan Amount		$ 115,000
Outstanding balance with OID		115,000
Original Issue Discount (OID)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 5,750
Discount for conversion feature and warrants/shares		$ 15,460
Convertible Notes [Member] | Convertible Debt Twelve [Member]
Inception Date	[1]	July 19, 2019
Term		12 months
Loan Amount		$ 130,000
Outstanding balance with OID		130,000
Original Issue Discount (OID)
Interest Rate		6.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 6,500
Discount for conversion feature and warrants/shares
Convertible Notes [Member] | Convertible Debt Thirteen [Member]
Inception Date	[1]	September 27, 2019
Term		12 months
Loan Amount		$ 78,750
Outstanding balance with OID		78,750
Original Issue Discount (OID)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 3,750
Discount for conversion feature and warrants/shares		$ 13,759
Convertible Notes [Member] | Convertible Debt Fourteen [Member]
Inception Date	[1]	October 24, 2019
Term		12 months
Loan Amount		$ 78,750
Outstanding balance with OID		78,750
Original Issue Discount (OID)
Interest Rate		4.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 3,750
Discount for conversion feature and warrants/shares
Convertible Notes [Member] | Convertible Debt Fifteen [Member]
Inception Date	[1]	November 1, 2019
Term		12 months
Loan Amount		$ 270,000
Outstanding balance with OID		270,000
Original Issue Discount (OID)
Interest Rate		6.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 13,500
Discount for conversion feature and warrants/shares
Convertible Notes [Member] | Convertible Debt Sixteen [Member]
Inception Date	[2]	November 15, 2019
Term		12 months
Loan Amount		$ 385,000
Outstanding balance with OID		320,000
Original Issue Discount (OID)		$ 35,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 35,000
Discount for conversion feature and warrants/shares		$ 90,917
Convertible Notes [Member] | Convertible Debt Seventeen [Member]
Inception Date	[2]	January 2, 2020
Term		12 months
Loan Amount		$ 330,000
Outstanding balance with OID		330,000
Original Issue Discount (OID)		$ 30,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 30,000
Discount for conversion feature and warrants/shares		$ 91,606
Convertible Notes [Member] | Convertible Debt Eighteen [Member]
Inception Date	[2]	January 24, 2020
Term		12 months
Loan Amount		$ 247,500
Outstanding balance with OID		247,500
Original Issue Discount (OID)		$ 22,500
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 22,500
Discount for conversion feature and warrants/shares		$ 89,707
Convertible Notes [Member] | Convertible Debt Nineteen [Member]
Inception Date	[2]	January 29, 2020
Term		12 months
Loan Amount		$ 363,000
Outstanding balance with OID		363,000
Original Issue Discount (OID)		$ 33,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 33,000
Discount for conversion feature and warrants/shares		$ 297,000
Convertible Notes [Member] | Convertible Debt Twenty [Member]
Inception Date	[2]	February 12, 2020
Term		12 months
Loan Amount		$ 275,000
Outstanding balance with OID		275,000
Original Issue Discount (OID)		$ 25,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 25,000
Discount for conversion feature and warrants/shares		$ 225,000
Convertible Notes [Member] | Convertible Debt Twenty One [Member]
Inception Date	[2]	February 19, 2020
Term		12 months
Loan Amount		$ 165,000
Outstanding balance with OID		165,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 15,000
Discount for conversion feature and warrants/shares		$ 135,000
Convertible Notes [Member] | Convertible Debt Twenty Two [Member]
Inception Date	[2]	March 11, 2020
Term		12 months
Loan Amount		$ 330,000
Outstanding balance with OID		330,000
Original Issue Discount (OID)		$ 30,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 30,000
Discount for conversion feature and warrants/shares		$ 232,810
Convertible Notes [Member] | Convertible Debt Twenty Three [Member]
Inception Date	[2]	March 13, 2020
Term		12 months
Loan Amount		$ 165,000
Outstanding balance with OID		165,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 15,000
Discount for conversion feature and warrants/shares		$ 60,705
Convertible Notes [Member] | Convertible Debt Twenty Four [Member]
Inception Date	[2]	March 26, 2020
Term		12 months
Loan Amount		$ 111,100
Outstanding balance with OID		111,100
Original Issue Discount (OID)		$ 10,100
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 10,100
Discount for conversion feature and warrants/shares		$ 90,900
Convertible Notes [Member] | Convertible Debt Twenty Five [Member]
Inception Date	[2]	April 8, 2020
Term		12 months
Loan Amount		$ 276,100
Outstanding balance with OID		276,100
Original Issue Discount (OID)		$ 25,100
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 25,000
Discount for conversion feature and warrants/shares		$ 221,654
Convertible Notes [Member] | Convertible Debt Twenty Six [Member]
Inception Date	[2]	April 17, 2020
Term		12 months
Loan Amount		$ 143,750
Outstanding balance with OID		143,750
Original Issue Discount (OID)		$ 18,750
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 96,208
Convertible Notes [Member] | Convertible Debt Twenty Seven [Member]
Inception Date	[2]	April 30, 2020
Term		12 months
Loan Amount		$ 546,250
Outstanding balance with OID		546,250
Original Issue Discount (OID)		$ 71,250
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 47,500
Discount for conversion feature and warrants/shares		$ 427,500
Convertible Notes [Member] | Convertible Debt Twenty Eight [Member]
Inception Date	[2]	May 6, 2020
Term		12 months
Loan Amount		$ 460,000
Outstanding balance with OID		460,000
Original Issue Discount (OID)		$ 60,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 40,000
Discount for conversion feature and warrants/shares		$ 360,000
Convertible Notes [Member] | Convertible Debt Twenty Nine [Member]
Inception Date		May 18, 2020
Term		12 months
Loan Amount		$ 546,250
Outstanding balance with OID		221,250
Original Issue Discount (OID)		$ 46,250
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 35,500
Discount for conversion feature and warrants/shares		$ 439,500
Convertible Notes [Member] | Convertible Debt Thirty [Member]
Inception Date		June 2, 2020
Term		12 months
Loan Amount		$ 902,750
Outstanding balance with OID		652,750
Original Issue Discount (OID)		$ 92,750
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 58,900
Discount for conversion feature and warrants/shares		$ 708,500
Convertible Notes [Member] | Convertible Debt Thirty One [Member]
Inception Date		June 12, 2020
Term		12 months
Loan Amount		$ 57,500
Outstanding balance with OID		57,500
Original Issue Discount (OID)		$ 7,500
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 5,000
Discount for conversion feature and warrants/shares		$ 45,000
Convertible Notes [Member] | Convertible Debt Thirty Two [Member]
Inception Date		June 22, 2020
Term		12 months
Loan Amount		$ 138,000
Outstanding balance with OID		138,000
Original Issue Discount (OID)		$ 18,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 12,000
Discount for conversion feature and warrants/shares		$ 108,000
Convertible Notes [Member] | Convertible Debt Thirty Three [Member]
Inception Date		July 7, 2020
Term		12 months
Loan Amount		$ 586,500
Outstanding balance with OID		586,500
Original Issue Discount (OID)		$ 76,500
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 51,000
Discount for conversion feature and warrants/shares		$ 400,234
Convertible Notes [Member] | Convertible Debt Thirty Four [Member]
Inception Date		July 17, 2020
Term		12 months
Loan Amount		$ 362,250
Outstanding balance with OID		362,250
Original Issue Discount (OID)		$ 47,250
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 31,500
Discount for conversion feature and warrants/shares		$ 185,698
Convertible Notes [Member] | Convertible Debt Thirty Five [Member]
Inception Date		July 29, 2020
Term		12 months
Loan Amount		$ 345,000
Outstanding balance with OID		345,000
Original Issue Discount (OID)		$ 45,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 30,000
Discount for conversion feature and warrants/shares		$ 241,245
Convertible Notes [Member] | Convertible Debt Thirty Six [Member]
Inception Date	[5]	July 21, 2020
Term		12 months
Loan Amount		$ 115,000
Outstanding balance with OID		115,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 10,000
Discount for conversion feature and warrants/shares		$ 24,875
Convertible Notes [Member] | Convertible Debt Thirty Seven [Member]
Inception Date		August 14, 2020
Term		12 months
Loan Amount		$ 762,450
Outstanding balance with OID		462,450
Original Issue Discount (OID)		$ 69,450
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 66,300
Discount for conversion feature and warrants/shares		$ 580,124
Convertible Notes [Member] | Convertible Debt Thirty Eight [Member]
Inception Date		September 10, 2020
Term		12 months
Loan Amount		$ 391,000
Outstanding balance with OID		391,000
Original Issue Discount (OID)		$ 51,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 34,000
Discount for conversion feature and warrants/shares		$ 231,043
Convertible Notes [Member] | Convertible Debt Thirty Nine [Member]
Inception Date	[5]	September 21, 2020
Term		12 months
Loan Amount		$ 345,000
Outstanding balance with OID		345,000
Original Issue Discount (OID)		$ 45,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 30,000
Discount for conversion feature and warrants/shares		$ 66,375
Convertible Notes [Member] | Convertible Debt Fourty [Member]
Inception Date	[5]	September 23, 2020
Term		12 months
Loan Amount		$ 115,000
Outstanding balance with OID		115,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 10,000
Discount for conversion feature and warrants/shares		$ 20,500
Convertible Notes [Member] | Convertible Debt Fourty One [Member]
Inception Date	[5]	September 25, 2020
Term		12 months
Loan Amount		$ 115,000
Outstanding balance with OID		115,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 19,125
Convertible Notes [Member] | Convertible Debt Fourty Two [Member]
Inception Date		December 3, 2020
Term		12 months
Loan Amount		$ 299,000
Outstanding balance with OID		299,000
Original Issue Discount (OID)		$ 39,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 26,000
Discount for conversion feature and warrants/shares		$ 197,882
Convertible Notes [Member] | Convertible Debt Fourty Three [Member]
Inception Date		December 21, 2020
Term		6 months
Loan Amount		$ 100,000
Outstanding balance with OID		100,000
Original Issue Discount (OID)		$ 5,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 29,964
Discount for conversion feature and warrants/shares		$ 24,400
Convertible Notes [Member] | Convertible Debt Fourty Four [Member]
Inception Date	[5]	October 22, 2020
Term		12 months
Loan Amount		$ 115,000
Outstanding balance with OID		115,000
Original Issue Discount (OID)		$ 15,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 10,000
Discount for conversion feature and warrants/shares		$ 18,875
Convertible Notes [Member] | Convertible Debt Fourty Five [Member]
Inception Date	[5]	December 23, 2020
Term		6 months
Loan Amount		$ 1,000,000
Outstanding balance with OID		1,000,000
Original Issue Discount (OID)		$ 100,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 833,536
Convertible Notes [Member] | Convertible Debt Forty Six [Member]
Inception Date		January 5, 2021
Term		6 months
Loan Amount		$ 575,000
Outstanding balance with OID		575,000
Original Issue Discount (OID)		$ 75,000
Interest Rate		18.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares
Convertible Notes [Member] | Convertible Debt Forty Seven [Member]
Inception Date		February 17, 2021
Term		12 months
Loan Amount		$ 230,000
Outstanding balance with OID		230,000
Original Issue Discount (OID)		$ 30,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees		$ 20,000
Discount for conversion feature and warrants/shares		$ 180,000
Convertible Notes [Member] | Convertible Debt Forty Eight [Member]
Inception Date		March 23, 2021
Term		12 months
Loan Amount		$ 55,000
Outstanding balance with OID		55,000
Original Issue Discount (OID)		$ 5,000
Interest Rate		10.00%
Conversion Price		$ 2.50
Deferred Finance Fees
Discount for conversion feature and warrants/shares		$ 36,431
[1]	As of March 31, 2021 the Company and lender have agreed to the extension of the Standstill and Forbearance agreements (as described below). Loan is convertible at $2.50 as of March 31, 2021.
[2]	The Note is past due. The Company and the lender are negotiating in good faith to extend the loan.
[3]	During the year ended December 31, 2020 the Company entered into Rate Modification Agreements with these lenders. In these agreements five lenders agreed to reduce their interest rate and were granted the right to convert loans using a variable conversion price if more than one other variable rate lender converted at a variable rate.
[4]	Interest was capitalized and added to outstanding principal.
[5]	The Company has agreed to issue shares of its common stock to lenders if their notes are not repaid by a defined date.

Convertible Debt and Other De_5

Convertible Debt and Other Debt - Schedule of Convertible Debts and Outstanding Balances (Details) (Parenthetical)	Mar. 31, 2021$ / shares
Standstill and Forbearance Agreements [Member]
Conversion Price	$ 2.50

Convertible Debt and Other De_6

Convertible Debt and Other Debt - Summary of Changes in Convertible Debt and Revolving Note Payable, Net of Unamortized Discounts (Details) - USD ($)	3 Months Ended
	Mar. 31, 2021	Mar. 31, 2020	Dec. 31, 2020
Debt Disclosure [Abstract]
Balance at January 1,	$ 7,545,670
Issuance of convertible debt, face value	860,000
Deferred financing cost	(130,000)
Beneficial conversion feature on convertible note	(53,777)
Debt discount from shares and warrants issued with debt	(162,654)
Payments	(191,250)	$ (520,500)
Conversion of debt into equity	(100,000)
Accretion of interest and amortization of debt discount to interest expense	2,124,788
Balance at March 31,	9,892,777
Less: current portion	9,892,777		$ 7,545,670
Convertible debt, long-term portion

Convertible Debt and Other De_7

Convertible Debt and Other Debt - Schedule of Merchant Agreements (Details) - USD ($)	3 Months Ended	12 Months Ended
	Mar. 31, 2021	Dec. 31, 2020
Purchase Price	$ 590,000	$ 300,000
Purchased Amount	801,360	413,700
Outstanding Balance	488,374	248,968
Daily Payment Rate	5,777	2,709
Deferred Finance Fees	$ 2,500
Merchant Agreements One [Member]
Inception Date	Nov. 19, 2020	Nov. 5, 2020
Purchase Price	$ 100,000	$ 200,000
Purchased Amount	137,900	275,800
Outstanding Balance	38,468	163,955
Daily Payment Rate	985	1,724
Deferred Finance Fees
Merchant Agreements Two [Member]
Inception Date	Feb. 4, 2021	Nov. 19, 2020
Purchase Price	$ 125,000	$ 100,000
Purchased Amount	165,000	137,900
Outstanding Balance	102,084	85,013
Daily Payment Rate	1,032	985
Deferred Finance Fees
Merchant Agreements Three [Member]
Inception Date	Mar. 11, 2021
Purchase Price	$ 125,000
Purchased Amount	167,500
Outstanding Balance	111,565
Daily Payment Rate	1,396
Deferred Finance Fees	$ 2,500
Merchant Agreements Four [Member]
Inception Date	Mar. 26, 2021
Purchase Price	$ 240,000
Purchased Amount	330,960
Outstanding Balance	236,257
Daily Payment Rate	2,364
Deferred Finance Fees

Stockholders' Deficit (Details

Stockholders' Deficit (Details Narrative) - USD ($)	Dec. 23, 2020	Mar. 31, 2021	Mar. 31, 2020	Dec. 31, 2020	Dec. 12, 2013
Common stock, shares outstanding under the plan		16,259,701		15,790,603
Total unrecognized compensation cost		$ 285,781
Unvested stock options weighted average period		1 year 8 months 5 days
Aggregate intrinsic value of options outstanding and exercisable		$ 1,298,874
Closing stock price		$ 2.10
Stock options, exercise price		$ 0.69
Stock options, exercisable		15,822,398		15,302,830
Loan issue description	We are obligated to issue 100,000 warrants if the loan is not repaid before January 23, 2021 and an additional 10,000 shares of common stock and 100,000 warrants if the loan is not repaid before February 23, 2021. We are also obligated to issue 10,000 shares of common stock and 200,000 warrants if the loan is not repaid before March 23, 2021. During the quarter ended March 31,2021 the Company issued 400,000 warrants to this lender ($3.50 exercise price and five year term) with a fair value of $600,298. The Company is also obligated to issue 10,000 shares of common stock to this lender every 31 days up to the loan’s maturity date on June 23, 2021.
Issuance of common stock for interest paid-in-kind		$ 2,021,780
Issuance of common stock for services, shares		112,400
Issuance of common stock for services		$ 238,512
Conversion of debt and interest for common stock, shares		47,200
Conversion of debt and interest for common stock		$ 118,000
Stock option exercise, shares		21,411
Warrants issued		521,200
Warrants term		5 years
Warrant exercise price per share		$ 3.50
Number of restricted stock issued		115,021
Common stock, fair value			$ 25,000
Lenders [Member]
Issuance of common stock for interest paid-in-kind		$ 922,372
Issuance of common stock for interest paid-in-kind, shares		2,000,000
Conversion of debt and interest for common stock		$ 800,000
Warrants issued		400,000
Warrants term		5 years
Fair value of warrants		$ 600,298
Warrant exercise price per share		$ 3.50
Accredited Investor and Consultants [Member]
Number of restricted stock issued during period, value			$ 213,415
Stock Option [Member]
Common stock, shares outstanding under the plan		1,358,490
Stock options, exercise price		$ 0.72
Stock options weighted average remaining term		8 years 5 months 20 days
Stock options, exercisable		921,187
2013 Equity Incentive Plan [Member]
Common stock, shares outstanding under the plan		1,358,490
2013 Equity Incentive Plan [Member]
Common stock reserved for stock option plan					3,000,000
Series AA Preferred Stock [Member]
Issuance of common stock for services, shares
Issuance of common stock for services
Number of common stock shares issued, shares
Common stock, fair value
Securities Purchase Agreement [Member] | Series AA Preferred Stock [Member]
Number of shares sold during period		40
Common stock, par value		$ 0.01
Sale of stock, purchase price		$ 100,000
Warrants issued		40,000
Warrant exercise price per share		$ 3.50
Conversion of common stock, shares		1,000
Stock Options Issuance [Member]
Stock options granted		24,000
Fair value of stock options granted		$ 49,135
Stock option, term		10 years
Stock options, exercise price		$ 2.17
Settled Accrued Liabilities [Member]
Number of restricted stock issued			66,500
Number of restricted stock issued during period, value			$ 127,855
Debt Extention and Interest Payments [Member]
Number of restricted stock issued			38,521
Number of restricted stock issued during period, value			$ 60,560
Debt Settlement [Member]
Number of restricted stock issued			10,000
Number of restricted stock issued during period, value			$ 25,000
Signing of New Convertible Loans [Member]
Warrants issued			1,095,230
Common stock, fair value			$ 1,205,010
Lenders for Debt Extension [Member]
Warrants issued			307,500
Common stock, fair value			$ 609,143
Preferred Stock [Member]
Convertible preferred stock, authorized		1,000,000
Convertible preferred stock, par value		$ 0.01
Convertible preferred stock, shares issued		1,000,000
Series A Junior Participating Preferred Stock [Member]
Convertible preferred stock, authorized		20,000
Convertible preferred stock, shares issued
Preferred stock, shares outstanding
Series A Convertible Preferred Stock [Member]
Convertible preferred stock, authorized		313,960
Convertible preferred stock, shares issued
Preferred stock, shares outstanding
Series B Convertible Preferred Stock [Member]
Convertible preferred stock, authorized		279,256
Convertible preferred stock, shares issued
Preferred stock, shares outstanding
Series C Convertible Preferred Stock [Member]
Convertible preferred stock, authorized		88,098
Convertible preferred stock, shares issued
Preferred stock, shares outstanding
Series D Convertible Preferred Stock [Member]
Convertible preferred stock, authorized		850		850
Convertible preferred stock, par value		$ 0.01		$ 0.01
Convertible preferred stock, shares issued		300		300
Preferred stock, shares outstanding		300		300
Series E Convertible Preferred Stock [Member]
Convertible preferred stock, authorized		500
Convertible preferred stock, shares issued
Preferred stock, shares outstanding
Series G Convertible Preferred Stock [Member]
Convertible preferred stock, authorized		240,000		240,000
Convertible preferred stock, par value		$ 0.01		$ 0.01
Convertible preferred stock, shares issued		80,570		80,570
Preferred stock, shares outstanding		80,570		80,570
Series H Convertible Preferred Stock [Member]
Convertible preferred stock, authorized		10,000		10,000
Convertible preferred stock, par value		$ 0.01		$ 0.01
Convertible preferred stock, shares issued		10,000		10,000
Preferred stock, shares outstanding		10,000		10,000
Series H2 Convertible Preferred Stock [Member]
Convertible preferred stock, authorized		21		21
Convertible preferred stock, par value		$ 0.01		$ 0.01
Convertible preferred stock, shares issued		21		21
Preferred stock, shares outstanding		21		21
Series J Convertible Preferred Stock [Member]
Convertible preferred stock, authorized		6,250		6,250
Convertible preferred stock, par value		$ 0.01		$ 0.01
Convertible preferred stock, shares issued		3,458		3,458
Preferred stock, shares outstanding		3,458		3,458
Series K Convertible Preferred Stock [Member]
Convertible preferred stock, authorized		15,000		15,000
Convertible preferred stock, par value		$ 0.01		$ 0.01
Convertible preferred stock, shares issued		6,880		6,880
Preferred stock, shares outstanding		6,880		6,880
Series AA Convertible Preferred Stock [Member]
Convertible preferred stock, authorized		10,000		10,000
Convertible preferred stock, par value		$ 0.01		$ 0.01
Convertible preferred stock, shares issued		8,083		8,043
Preferred stock, shares outstanding		8,083		8,043

Stockholders' Deficit - Schedul

Stockholders' Deficit - Schedule of Concerning Options and Warrants Outstanding and Exercisable (Details)	3 Months Ended
	Mar. 31, 2021$ / sharesshares
Shares, Beginning balance	15,790,603
Shares, Granted	585,200
Shares, Exercised	(21,411)
Shares, Expired	(94,691)
Shares, Ending balance	16,259,701
Exercisable, Beginning balance	15,302,830
Exercisable, Ending balance	15,822,398
Stock Option [Member]
Shares, Beginning balance	1,355,901
Shares, Granted	24,000
Shares, Exercised	(21,411)
Shares, Expired
Shares, Ending balance	1,358,490
Weighted average price per share, Beginning balance | $ / shares	$ 0.69
Weighted average price per share, Granted | $ / shares	2.17
Weighted average price per share, Exercised | $ / shares	0.69
Weighted average price per share, Expired | $ / shares
Weighted average price per share, Ending balance | $ / shares	$ 0.69
Warrants [Member]
Shares, Beginning balance	14,434,702
Shares, Granted	561,200
Shares, Exercised
Shares, Expired	(94,691)
Shares, Ending balance	14,901,211
Weighted average price per share, Beginning balance | $ / shares	$ 3.50
Weighted average price per share, Granted | $ / shares	3.50
Weighted average price per share, Exercised | $ / shares
Weighted average price per share, Expired | $ / shares	3.50
Weighted average price per share, Ending balance | $ / shares	$ 3.50

Stockholders' Deficit - Sched_2

Stockholders' Deficit - Schedule of Loans Obligated to Issue Shares (Details) - USD ($)	Dec. 23, 2020	Mar. 31, 2021
Loan Principal		$ 860,000
Defined Frequency	We are obligated to issue 100,000 warrants if the loan is not repaid before January 23, 2021 and an additional 10,000 shares of common stock and 100,000 warrants if the loan is not repaid before February 23, 2021. We are also obligated to issue 10,000 shares of common stock and 200,000 warrants if the loan is not repaid before March 23, 2021. During the quarter ended March 31,2021 the Company issued 400,000 warrants to this lender ($3.50 exercise price and five year term) with a fair value of $600,298. The Company is also obligated to issue 10,000 shares of common stock to this lender every 31 days up to the loan’s maturity date on June 23, 2021.
Loans 1 [Member]
Loan Issuance Date		Jul. 21, 2020
Loan Principal		$ 115,000
Shares Issuable		5,000
Defined Date		Sep. 30, 2020
Defined Frequency		Monthly
Loans 2 [Member]
Loan Issuance Date		Sep. 21, 2020
Loan Principal		$ 345,000
Shares Issuable		12,500
Defined Date		Nov. 16, 2020
Defined Frequency		Weekly
Loans 3 [Member]
Loan Issuance Date		Sep. 23, 2020
Loan Principal		$ 115,000
Shares Issuable		12,500
Defined Date		Dec. 1, 2020
Defined Frequency		Weekly
Loans 4 [Member]
Loan Issuance Date		Sep. 25, 2020
Loan Principal		$ 115,000
Shares Issuable		12,500
Defined Date		Dec. 1, 2020
Defined Frequency		Weekly
Loans 5 [Member]
Loan Issuance Date		Oct. 22, 2020
Loan Principal		$ 115,000
Shares Issuable		12,500
Defined Date		Dec. 1, 2020
Defined Frequency		Weekly

Subsequent Events (Details Narr

Subsequent Events (Details Narrative) - USD ($)	Dec. 23, 2020	May 05, 2021	Mar. 31, 2021	Mar. 31, 2020
Warrants issued			521,200
Warrants term			5 years
Warrant exercise price per share			$ 3.50
Proceeds from related party loan			$ 85,000	$ 8,500
Debt description	We are obligated to issue 100,000 warrants if the loan is not repaid before January 23, 2021 and an additional 10,000 shares of common stock and 100,000 warrants if the loan is not repaid before February 23, 2021. We are also obligated to issue 10,000 shares of common stock and 200,000 warrants if the loan is not repaid before March 23, 2021. During the quarter ended March 31,2021 the Company issued 400,000 warrants to this lender ($3.50 exercise price and five year term) with a fair value of $600,298. The Company is also obligated to issue 10,000 shares of common stock to this lender every 31 days up to the loan’s maturity date on June 23, 2021.
Issuance of common stock for interest paid-in-kind			$ 2,021,780
Merchant Agreements [Member]
Repayments of debt	$ 300,000
Issuance of common stock for interest paid-in-kind, shares	331,370
Issuance of common stock for interest paid-in-kind	$ 671,066
Subsequent Event [Member]
Proceeds from loans		$ 747,500
Warrants issued		299,000
Warrants term		5 years
Warrant exercise price per share		$ 3.50
Debt, conversion price per share		$ 2.50
Debt, interest rate		10.00%
Subsequent Event [Member] | New Related Party [Member]
Proceeds from related party loan		$ 50,000
Debt description		Merchant Cash lender agreement (collecting $200,000 of which $27,580 was used to settle an existing merchant agreement dated November 19, 2020). Under the Merchant Cash agreement, the Company pays $1,970 each business day to the Merchant Cash lender until the lender has received cumulative payments of $275,800.