69 patents
Page 2 of 4
Utility
Compositions and Methods for Genomic Integration of Nucleic Acids into Exogenous Landing Pads
11 Aug 22
Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome.
Kevin GEORGE, Andrew MAIN, Chia-Hong TSAI
Filed: 9 Mar 22
Utility
Methods for genomic integration in pichia and other host cells
19 Jul 22
The present invention provides high efficiency targeted and marker-less single, double, triple, quadruple, and quintuple integrations by using CRISPR in host cells, including Pichia.
Hanxiao Jiang
Filed: 12 Sep 18
Utility
Methods for genomic integration
19 Jul 22
Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. in certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. in some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.
Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
Filed: 11 Mar 20
Utility
Microbial Production of Compounds
28 Apr 22
Provide are modified host cells that are engineered to decrese expression of a product to undetectable levels in the presence of an exogenous agent, and increase expression of the product in the presence of another exogenous agent.
Christopher D. REEVES, Christopher J. PADDON, Victor F. HOLMES, Andrew P. KLEIN
Filed: 13 Mar 20
Utility
Methods for recovering water-immiscible isoprenoid compounds from microbial biomass
26 Apr 22
A method for recovering one or more water immiscible compounds comprising acidifying and disrupting the microbial biomass; heating the acidified, disrupted microbial biomass to form a heated, acidified disrupted microbial biomass; and contacting the heated, acidified, disrupted microbial biomass with a disulfonated surfactant in an amount sufficient to release at least 30% of the one or more water immiscible compounds from the microbial biomass.
Ronald Ray Henry, Joshua Steven Leng
Filed: 27 Jun 19
Utility
Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
5 Apr 22
Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome.
Kevin George, Andrew Main, Chia-Hong Tsai
Filed: 18 May 17
Utility
Methods for stabilizing production of acetyl-coenzyme A derived compounds
25 Jan 22
The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells.
Penelope R. Chua, Adam Meadows
Filed: 2 Jan 20
Utility
Compositions and methods for delivering cannabinoids to skin
11 Jan 22
Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject.
Diva Chan, Ro Oteri, Ashlee Nunes
Filed: 14 Jul 21
Utility
Compositions and Methods for Production of Myrcene
6 Jan 22
Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase.
Andrew MAIN, Grzegorz WOJCIECHOWSKI, Yue YANG, Lishan ZHAO
Filed: 9 Jul 21
Utility
Compositions and methods for delivering cannabinoids to skin
4 Jan 22
Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject.
Diva Chan, Ro Oteri, Ashlee Nunes
Filed: 19 Mar 21
Utility
Olefins and methods for making the same
7 Dec 21
Provided herein are olefinic feedstocks derived from conjugated hydrocarbon terpenes (e.g., C10-C30 terpenes), methods for making the same, and methods for their use.
Nicholas Ohler, Karl Fisher, Jin Ki Hong
Filed: 19 Apr 19
Utility
Stevia Rebaudiana Kaurenoic Acid Hydroxylase Variants for High Efficiency Production of Rebaudiosides
2 Dec 21
Provided herein are compositions and methods for improved production of steviol glycosides in a host cell.
Gale A. WICHMANN, Wenzong LI, Sean LUND, Shaina J. JACKSON, Chantal V. GARCIA DE GONZALO, Hailley WARBINGTON, Yi XIONG, Svetlana Alekseyevna BORISOVA
Filed: 14 Oct 19
Utility
Udp-dependent Glycosyltransferase for High Efficiency Production of Rebaudiosides
18 Nov 21
Provided herein are compositions and methods for improved production of steviol glycosides in a host cell.
Lishan ZHAO, Wenzong LI, Gale WICHMANN, Aditi KHANKHOJE, Chantal GARCIA DE GONZALO, Tina MAHATDEJKUL-MEADOWS, Shaina JACKSON, Michael LEAVELL, Darren PLATT
Filed: 22 Jul 21
Utility
Compositions and Methods for Delivering Cannabinoids to Skin
4 Nov 21
Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject.
Diva CHAN, Ro OTERI, Ashlee NUNES
Filed: 14 Jul 21
Utility
Process for recovering isoprenoids produced by microorganisms
31 Aug 21
Maha Bahi, Anthony Hutin, Fernando Leal-Calderon
Filed: 1 Aug 18
Utility
UDP-dependent glycosyltransferase for high efficiency production of rebaudiosides
17 Aug 21
Provided herein are compositions and methods for improved production of steviol glycosides in a host cell.
Lishan Zhao, Wenzong Li, Gale Wichmann, Aditi Khankhoje, Chantal Garcia De Gonzalo, Tina Mahatdejkul-Meadows, Shaina Jackson, Michael Leavell, Darren Platt
Filed: 11 Aug 17
Utility
Compositions and methods for production of myrcene
10 Aug 21
Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyrophosphate synthase.
Andrew Main, Grzegorz Wojciechowski, Yue Yang, Lishan Zhao
Filed: 28 Oct 16
Utility
Maltose Dependent Degrons, Maltose-responsive Promoters, Stabilization Constructs, and Their Use In Production of Non-catabolic Compounds
29 Jul 21
The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level.
Penelope R. CHUA, Hanxiao JIANG, Adam Leon MEADOWS
Filed: 2 Apr 21
Utility
Methods for Controlling Fermentation Feed Rates
29 Jul 21
The present disclosure relates to methods for enhancing productivity or yield in fermentation.
Joshua LENG
Filed: 11 Jul 19
Utility
Methods for Recovering Water-immiscible Isoprenoid Compounds from Microbial Biomass
29 Jul 21
A method for recovering one or more water immiscible compounds comprising acidifying and disrupting the microbial biomass; heating the acidified, disrupted microbial biomass to form a heated, acidified disrupted microbial biomass; and contacting the heated, acidified, disrupted microbial biomass with a disulfonated surfactant in an amount sufficient to release at least 30% of the one or more water immiscible compounds from the microbial biomass.
Ronald Ray HENRY, Joshua Steven LENG
Filed: 27 Jun 19