Exhibit 21.1 SUBSIDIARIES
| Subsidiary | Jurisdiction of Organization | |
| 1 | Charles River Laboratories, Inc. | Delaware |
| 2 | Charles River UK Limited | United Kingdom (England) |
| 3 | Charles River Laboratories Saint‑Constant S.A. | Quebec, Canada |
| 4 | Charles River Holdings LLC | Delaware |
| 5 | Ballardvale CV | Netherlands |
| 6 | Charles River Nederland BV | Netherlands |
| 7 | Charles River Laboratories Holding SAS | France |
| 8 | Charles River Laboratories France—C.R.L.F. SAS | France |
| 9 | Charles River Laboratories Belgium SPRL | Belgium |
| 10 | Charles River Laboratories Espana SA | Spain |
| 11 | Charles River Laboratories Japan, Inc. | Japan |
| 12 | Charles River Germany Verwaltungs GmbH | Germany |
| 13 | Charles River Laboratories Italia Srl | Italy |
| 14 | Charles River Germany GmbH & Co. KG | Germany |
| 15 | Charles River Laboratories Preclinical Services Poland Sp. Z.o.o. | Poland |
| 16 | Charles River Laboratories Preclinical Services Ireland Limited | Ireland |
| 17 | Entomology Europe Limited | Ireland |
| 18 | Saothorlanna Bitheolaiocha Idirnaisiunta Teoranta | Ireland |
| 19 | Charles River Laboratories, Research Models and Services, Germany GmbH | Germany |
| 20 | Charles River Laboratories Luxembourg S.a.r.l. | Luxembourg |
| 21 | Charles River Laboratories Group | United Kingdom (Scotland) |
| 22 | Charles River Laboratories Holdings Scotland | United Kingdom (England) |
| 23 | Charles River Laboratories Preclinical Services Edinburgh Ltd. | United Kingdom (Scotland) |
| 24 | Charles River Clinical Services Edinburgh Ltd. | United Kingdom (Scotland) |
| 25 | Inveresk Research (Canada) ULC | Nova Scotia, Canada |
| 26 | Charles River Laboratories Preclinical Services Montreal, ULC | Nova Scotia, Canada |
| 27 | Charles River Laboratories Australia Pty. Ltd. | Australia |
| 28 | Zhanjiang A&C Biological Ltd. | China |
| 29 | Charles River Laboratories Korea | Korea |
| 30 | Charles River Laboratories Asia Holdings Limited | Hong Kong |
| 31 | Charles River Laboratories Preclinical Services Hong Kong Limited | Hong Kong |
| 32 | Charles River Laboratories Greater China Preclinical Services Shanghai Co. Ltd. | China |
| 33 | Charles River Biopharmaceutical Services GmbH | Germany |
| 34 | Charles River Discovery Research Services International, Inc. | Michigan |
| 35 | Charles River Discovery Research Services, Inc. | Michigan |
| 36 | Charles River Laboratories India Private Limited | India |
| 37 | Charles River Discovery Research Services Finland | Finland |
| 38 | Systems Pathology Company, LLC | Delaware |
| 39 | Charles River USA Holdings LLC | Delaware |
| 40 | Accugenix Inc. | Delaware |
| 41 | Beijing Vital River Laboratory Animal Technology Co. Ltd. | China |
| 42 | Accugenix GmbH | Germany |
| 43 | Charles River Endotoxin and Microbial Detection Singapore Pte. Ltd. | Singapore |
| 44 | Charles River Laboratories Bangladesh Ltd. | Bangladesh |
| 45 | Charles River Endotoxin Microbial Detection Europe SAS | France |