Exhibit 21.1
| Subsidiaries of Hologic | Jurisdiction of Incorporation or Organization | |
| Beijing Hologic Technology Co., Ltd. | China | |
| BioLucent, LLC | Delaware | |
| Cytyc Corporation | Delaware | |
| Direct Radiography Corp. | Delaware | |
| Gen-Probe Incorporated | Delaware | |
| Hologic Asia, Limited | Hong Kong | |
| Hologic (China) Enterprise Management Consulting Co., Ltd. | China | |
| Hologic Hitec-Imaging GmbH | Germany | |
| Hologic International Holdings B.V. | Netherlands | |
| Hologic Latin America Ltda. | Brazil | |
| Interlace Medical, Inc. | Delaware | |
| Navigation Three Limited | Hong Kong | |
| R2 Technology Canada, Inc. | Canada | |
| Sentinelle Medical Inc. | Canada | |
| Suros Surgical Systems, Inc. | Delaware | |
| Third Wave Technologies, Inc. | Delaware | |
| TCT International Co., Ltd. | British Virgin Islands | |
| Hologic España S.A. | Spain | |
| Hologic France S.A. | France | |
| Hologic N.V. | Belgium | |
| Third Wave Agbio, Inc. | Delaware | |
| Hologic Japan, Inc. | Japan | |
| Hologic Medical Technologies (Beijing) Co., Ltd. | China | |
| Cruiser, Inc. | Delaware | |
| Cytyc Limited Liability Company | Delaware | |
| Cytyc International, Inc. | Delaware | |
| Cytyc Prenatal Products Corp. | Delaware | |
| Hologic Limited Partnership | Massachusetts | |
| Cytyc Surgical Products, Limited Partnership | Massachusetts | |
| Cytyc Surgical Products II, Limited Partnership | Massachusetts | |
| Hologic Surgical Products Costa Rica, S.A. | Costa Rica | |
| Cytyc Cayman Limited | Cayman Islands | |
| Hologic Asia Pacific Limited | Hong Kong | |
| Hologic Netherlands B.V. | Netherlands | |
| Hologic Canada Limited | Canada | |
| Hologic (Australia) Pty Ltd. | Australia | |
| Hologic Deutschland, GmbH | Germany | |
| Hologic Europe Middle East and Africa, S.A. | Switzerland | |
| Hologic France SARL | France | |
| Hologic Iberia, S.L. | Spain | |
| Hologic Italia S.r.l. | Italy | |
| Hologic (UK) Limited | England and Wales | |
| Hologic Suisse SA | Switzerland | |
| Gen-Probe Prodesse, Inc. | Wisconsin | |
| Subsidiaries of Hologic | Jurisdiction of Incorporation or Organization | |
| Gen-Probe Sales & Service, Inc. | Delaware | |
| Gen-Probe Australia Pty Ltd. | Australia | |
| Gen-Probe Czech Republic s.r.o. | Czech Republic | |
| Hologic Denmark ApS | Denmark | |
| Hologic Ltd. | United Kingdom | |
| Hologic Sweden AB | Sweden | |
| Gen-Probe UK Limited | United Kingdom | |
| Food DNA Services Limited | United Kingdom | |
| Tepnel Biosystems Limited | United Kingdom | |
| Tepnel Diagnostics Limited | United Kingdom | |
| Tepnel Medical Limited | United Kingdom | |
| Tepnel Scientific Services Limited | United Kingdom | |
| Wildlife DNA Services Limited | United Kingdom | |
| Molecular Light Technology Limited | United Kingdom | |
| Bioanalysis Limited | United Kingdom | |
| Gen-Probe Cardiff Ltd. | United Kingdom | |
| Beijing TCT Jinbai Technologies Co., Ltd. | China | |
| Jiangsu Kang Ke Medical Devices Co., Ltd. | China | |
| Zheng Zhou Yong Run Medical Devices Co., Ltd. | China | |
| TCT Medical (Beijing) Clinical Test Institute | China | |
| Hangzhou Zuanbai Technology Co., Ltd | China | |
| Mingwood Biotechnology Co., Ltd. | China | |
| Century Likang (Beijing) Co., Ltd. | China | |
| Beijing TCT Medical Technology Co., Ltd. | China | |