159 patents
Page 8 of 8
Utility
Modular Universal Plasmid Design Strategy for the Assembly and Editing of Multiple Dna Constructs for Multiple Hosts
22 Apr 20
The disclosure describes methods, compositions, and kits for high throughput DNA assembly reactions in vitro.
Brian Chaikind, Hendrik Marinus van Rossum
Filed: 7 Aug 19
Utility
a High-throughput (HTP) Genomic Engineering Platform for Improving Saccharopolyspora Spinosa
15 Apr 20
The present disclosure provides a HTP microbial genomic engineering platform for Saccharopolyspora spp. that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols.
Benjamin MASON, Alexi GORANOV, Peter KELLY, Youngnyun KIM, Sheetal MODI, Nihal PASUMARTHI, Benjamin MIJTS, Peter ENYEART
Filed: 5 Jun 18
Utility
High Throughput Transposon Mutagenesis
1 Apr 20
The present disclosure is directed to a method of high-throughput (HTP) microbial genomic engineering, which utilizes in vivo transposon mutagenesis to develop strain libraries for the perturbation of microbial phenotypes.
Peter Kelly, Peter ENYEART
Filed: 5 Jun 18
Utility
HTP Genomic Engineering Platform for Improving Fungal Strains
4 Mar 20
The present disclosure provides a HTP genomic engineering platform for improving filamentous fungal cells that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols.
Vytas Sunspiral, Jennifer Fredlund, Hassan Abdulla, Paolo Boccazzi, Sean Poust, Sara da Luz Areaosa Cleto, Brian Chaikind, Dylan Vaughan, Kenneth S. Bruno, Patrick Westfall, Edyta Szewczyk, Kyle Rothschild-Mancinelli, Arthur Muir FONG, III
Filed: 10 Oct 19
Utility
Bioreachable Prediction Tool for Predicting Properties of Bioreachable Molecules and Related Materials
19 Feb 20
Systems, methods and computer-readable media are provided to predict properties of a material that is related to a bioreachable molecule by generating a chemical model of the material based on physicochemical properties and predicting properties of the material based at least in part upon the chemical model and correlative modeling.
Erik Jedediah Dean, Vanessa Blue Oklejas, Alexander Glennon Shearer, Vytas SunSpiral, Michelle L. Wynn, Lucas Andrew Zulauf
Filed: 14 Aug 19
Utility
APPLICATIONS OF CRISPRi IN HIGH THROUGHPUT METABOLIC ENGINEERING
19 Feb 20
The disclosure describes methods, compositions, and kits for high throughput DNA assembly reactions in vitro.
Brian Chaikind, Hendrik M. Van Rossum, Aaron Miller, Paul Perkovich, Shawn Szyjka, Kedar Patel
Filed: 14 Aug 19
Utility
Microbial Strain Improvement by a HTP Genomic Engineering Platform
12 Feb 20
The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols.
Zach Serber, Erik Jedediah Dean, Shawn Manchester, Katherine Gora, Michael Flashman, Erin Shellman, Aaron Kimball, Shawn Szyjka, Barbara Frewen, Thomas Treynor, Kenneth S. Bruno
Filed: 27 Oct 19
Utility
Liquid Based Selection and Cell Isolation
29 Jan 20
Aspects of this disclosure relate to liquid-based workflows for strain engineering.
Sean Poust, Vytas SunSpiral, William Serber, Matthew Jonathan Myers, Sara da Luz Areosa Cleto, Philip Weyman
Filed: 1 Jul 19
Utility
Methods for generating a bacterial hemoglobin library and uses thereof
27 Jan 20
The present disclosure describes methods for generating microbial strains expressing a heterologous bacterial hemoglobin gene that produce biomolecules of interest.
Shawn Manchester, Alexander Neckelmann
Filed: 27 Jun 17
Utility
Methods for generating a glucose permease library and uses thereof
27 Jan 20
The present disclosure describes methods for generating microbial strains expressing a heterologous bacterial glucose permease gene that produce biomolecules of interest.
Shawn Manchester, Jeffrey Mellin
Filed: 28 Jun 17
Utility
Bioreachable Prediction Tool
25 Dec 19
Systems and methods for predicting the viability of producing target molecules in a host organism are provided.
Erik Jedediah Dean, Alexander G. Shearer, Michelle L. Wynn
Filed: 11 Aug 19
Utility
Engineered Biosynthetic Pathways for Production of Tyramine by Fermentation
25 Dec 19
The present disclosure describes the engineering of microbial cells for fermentative production of tyramine and provides novel engineered microbial cells and cultures, as well as related tyramine production methods.
Michael Shareef Siddiqui, Stefan de Kok, Alexander Glennon Shearer, Franklin Lu, Cara Ann Tracewell, Steven M. Edgar
Filed: 25 Jun 19
Utility
Nepetalactol Oxidoreductases, Nepetalactol Synthases, and Microbes Capable of Producing Nepetalactone
25 Dec 19
The present disclosure provides isolated nepetalactone oxidoreductase polypeptides (NORs), nepetalactol synthases (NEPSs), and related polynucleotides, engineered host cells, and cultures, as well as methods for producing NORs and NEPSs, and for using them to produce nepetalactol, nepetalactone, and dihydronepetalactone.
Grayson Wawrzyn, Christine Roche, Patrick J. Westfall, Warren Lau, Savita Ganesan, Fern R. McSorley, Zach Serber
Filed: 6 Aug 19
Utility
Manipulation of Genes Involved In Signal Transduction to Control Fungal Morphology During Fermentation and Production
11 Dec 19
The present disclosure provides a microbial genomic engineering method and system for transforming, screening, and selecting filamentous fungal cells that have altered morphology and/or growth under specific growth conditions.
Kenneth S. Bruno, Sachin Jain, Brandon Pfannenstiel, Edyta Szewczyk
Filed: 5 Jun 19
Utility
Engineered Biosynthetic Pathways for Production of (6E)-8-HYDROXYGERANIOL by Fermentation
6 Nov 19
The present disclosure describes the engineering of microbial cells for fermentative production of (6E)-8-hydroxygeraniol and provides novel engineered microbial cells and cultures, as well as related (6E)-8-hydroxygeraniol production method.
Cara Ann Tracewell, Steven M. Edgar, Stepan Tymoshenko, Alexander Glennon Shearer
Filed: 23 Jun 19
Utility
Scarless Dna Assembly and Genome Editing Using CRISPR/CPF1 and Dna Ligase
30 Oct 19
The disclosure describes a scarless DNA assembly and genome editing methodology termed “CLIC” (CRISPR and Ligase Cloning), which utilizes a CRISPR/Cpf1 complex and DNA ligase to perform programmable gene editing and nucleotide assembly.
William C. DeLoache, Hendrik Marinus van Rossum, Kedar Gautam Patel
Filed: 13 Jul 17
Utility
Microbial strain improvement by a HTP genomic engineering platform
28 Oct 19
The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols.
Zach Serber, Erik Jedediah Dean, Shawn Manchester, Katherine Gora, Michael Flashman, Erin Shellman, Aaron Kimball, Shawn Szyjka, Barbara Frewen, Thomas Treynor, Kenneth S. Bruno
Filed: 15 Mar 18
Utility
Method to Build Fungal Production Strains Using Automated Steps for Genetic Manipulation and Strain Purification
23 Oct 19
The present disclosure provides a high-throughput (HTP) microbial genomic engineering method and system for transforming, screening, and selecting filamentous fungal cells that utilizes automation.
Kenneth S. BRUNO, Patrick WESTFALL, Edyta SZEWCZYK, Kyle ROTHSCHILD-MANCINELLI
Filed: 25 Jun 19
Utility
Microbial Strain Improvement by a HTP Genomic Engineering Platform
16 Oct 19
The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols.
Zach Serber, Erik Jedediah Dean, Shawn Manchester, Katherine Gora, Michael Flashman, Erin Shellman, Aaron Kimball, Shawn Szyjka, Barbara Frewen, Thomas Treynor, Kenneth S. Bruno
Filed: 30 Jun 19