Enumeral Biomedical 424B3 Prospectus supplement

Filed Pursuant to Rule 424(b)(3) and Rule 424(c)
Registration No. 333-198847

Prospectus Supplement No. 18
(To Prospectus filed on July 6, 2015, as supplemented
by Prospectus Supplement No. 1 dated July 14, 2015, Prospectus Supplement No. 2 dated July 28, 2015, Prospectus Supplement No. 3 dated August 4, 2015, Prospectus Supplement No. 4 dated August 12, 2015, Prospectus Supplement No. 5 dated September 17, 2015, Prospectus Supplement No. 6 dated September 18, 2015, Prospectus Supplement No. 7 dated September 24, 2015, Prospectus Supplement No. 8 dated September 25, 2015, Prospectus Supplement No. 9 dated September 30, 2015, Prospectus Supplement No. 10 dated October 2, 2015, Prospectus Supplement No. 11 dated November 3, 2015, Prospectus Supplement No. 12 dated November 10, 2015, Prospectus Supplement No. 13 dated November 18, 2015, Prospectus Supplement No. 14 dated December 1, 2015, Prospectus Supplement No. 15 dated December 8, 2015, Prospectus Supplement No. 16 dated January 8, 2016, and Prospectus Supplement No. 17

dated January 11, 2016)

ENUMERAL BIOMEDICAL HOLDINGS, INC.

This Prospectus Supplement No. 18 supplements the information contained in the Prospectus, dated as of July 6, 2015, as amended by Prospectus Supplement No. 1 dated July 14, 2015, Prospectus Supplement No. 2 dated July 28, 2015, Prospectus Supplement No. 3 dated August 4, 2015, Prospectus Supplement No. 4 dated August 12, 2015, Prospectus Supplement No. 5 dated September 17, 2015, Prospectus Supplement No. 6 dated September 18, 2015, Prospectus Supplement No. 7 dated September 24, 2015, Prospectus Supplement No. 8 dated September 25, 2015, Prospectus Supplement No. 9 dated September 30, 2015, Prospectus Supplement No. 10 dated October 2, 2015, Prospectus Supplement No. 11 dated November 3, 2015, Prospectus Supplement No. 12 dated November 10, 2015, Prospectus Supplement No. 13 dated November 18, 2015, Prospectus Supplement No. 14 dated December 1, 2015, Prospectus Supplement No. 15 dated December 8, 2015, Prospectus Supplement No. 16 dated January 8, 2016, and Prospectus Supplement No. 17 dated January 11, 2016, relating to the resale of up to 52,154,760 shares of our common stock by selling stockholders.

This Prospectus Supplement No. 18 is being filed to include the information set forth in our Current Report on Form 8-K, which was filed with the Securities and Exchange Commission on March 11, 2016.

You should read this Prospectus Supplement No. 18 in conjunction with the Prospectus. This Prospectus Supplement No. 18 is qualified by reference to the Prospectus, except to the extent that the information contained in this Prospectus Supplement No. 18 supersedes the information contained in the Prospectus. This Prospectus Supplement No. 18 is not complete without, and may not be utilized except in connection with, the Prospectus.

You should consider carefully the risks that we have described in “Risk Factors” beginning on page 7 of the Prospectus.

Neither the Securities and Exchange Commission nor any state securities commission has approved or disapproved of these securities or determined if this prospectus is truthful or complete. Any representation to the contrary is a criminal offense.

The date of this Prospectus Supplement is March 11, 2016

UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

FORM 8-K

CURRENT REPORT

Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934

Date of Report (Date of earliest event reported):March 11, 2016

Enumeral Biomedical Holdings, Inc.

(Exact name of registrant as specified in its charter)

Delaware	000-55415	99-0376434
(State or Other Jurisdiction	(Commission File	(I.R.S. Employer
of Incorporation)	Number)	Identification Number)

200 CambridgePark Drive, Suite 2000

Cambridge, Massachusetts

(Address of Principal Executive Offices)

02140

(Zip Code)

(617) 945-9146

(Registrant’s telephone number, including area code)

N/A

(Former name or former address, if changed since last report)

Check the appropriate box below if the Form 8-K filing is intended to simultaneously satisfy the filing obligation of the registrant under any of the following provisions:

¨ Written communications pursuant to Rule 425 under the Securities Act (17 CFR 230.425)

¨ Soliciting material pursuant to Rule 14a-12 under the Exchange Act (17 CFR 240.14a-12)

¨ Pre-commencement communications pursuant to Rule 14d-2(b) under the Exchange Act (17 CFR 240.14d-2(b))

¨ Pre-commencement communications pursuant to Rule 13e-4(c) under the Exchange Act (17 CFR 240.13e-4(c))

Item 7.01 Regulation FD Disclosure.

Enumeral Biomedical Holdings, Inc. (the “Company”) may use a slide presentation, in whole or in part, from time to time in presentations to potential partners, investors, analysts and others. A copy of the slide presentation is furnished as Exhibit 99.1 to this Current Report on Form 8-K and incorporated by reference herein. A copy of the slide presentation is also available on the Company’s website atwww.enumeral.com.

The information in this Item 7.01 of this Current Report on Form 8-K and in the accompanying Exhibit 99.1 shall not be deemed to be “filed” for purposes of Section 18 of the Securities Exchange Act of 1934, as amended, or otherwise subject to the liabilities of that Section or Sections 11 and 12(a)(2) of the Securities Act of 1933, as amended. The information contained in this Item 7.01 of this Current Report on Form 8-K and in the accompanying Exhibit 99.1 shall not be incorporated by reference into any filing with the U.S. Securities and Exchange Commission made by the Company, whether made before or after the date hereof, regardless of any general incorporation language in such filing.

Item 9.01 Financial Statements and Exhibits.

(d) Exhibits

Exhibit

Number

Description

99.1

Enumeral Biomedical Holdings, Inc. March 2016 Corporate Overview Presentation

SIGNATURES

Pursuant to the requirements of the Securities Exchange Act of 1934, the registrant has duly caused this report to be signed on its behalf by the undersigned hereunto duly authorized.

	ENUMERAL BIOMEDICAL HOLDINGS, INC.

Dated: March 11, 2016	By:	/s/ Kevin G. Sarney
		Name: Kevin G. Sarney
		Title: Vice President of Finance, Chief Accounting Officer and Treasurer

EXHIBIT INDEX

Exhibit No.		Description

99.1		Enumeral Biomedical Holdings, Inc. March 2016 Corporate Overview Presentation

Exhibit 99.1

Corporate Overview March 2016

Company Highlights • Enumeral is an antibody immuno - oncology company based on technology developed at Harvard and MIT • Enumeral’s differentiated PD - 1 antagonist C8 has potential to become best - in - class cancer immunotherapy – Novel mechanism of binding leads to in vitro enhanced activity – Strong IP Position – Fast - to - market development strategy – may address currently untreated patients • Platform - driven pipeline drives next - generation immunotherapy – Poised to rapidly develop antibodies to additional important targets – Anti - TIM3 and anti - LAG3 antibody programs cover large diversity of potential structures – Translational approach employing primary human tumor biopsy to determine rational combinations 2

Agenda 1. The Opportunity for Best - in - Class PD - 1 Antagonist 2. ENUM C8 – A Differentiated Anti - PD - 1 Antibody That Drives Adaptive and Innate Immune Cell Activation 3. TIM - 3 – Next Program; Additional Pipeline Programs 4. Clinical Opportunity and Development Plan 5. Platform and IP 6. Collaborators, Management, Directors 3

Large Market Opportunity Exists For A More Effective PD - 1 Directed Therapy 4 Non responders Responders Value to payors if new products treat prior therapy failures and relapse Estimated sales for checkpoint blockers in 2020 $27 billion per year* (Estimate only for PD - 1/PD - L1 class ) *Cowen and Company **Bristol Myers Squibb, Merck Sales of approved I/O drugs (1 st launched in 2011) $2.8 billion in annualized revenue (Quarter ended September 30, 2015**) Eligible patients

Limitations of 1 st generation PD - 1 inhibitors PD - 1 blockade through disrupting the PD - L1 interaction is an incomplete solution • In the approved indications, response rates are around 20% • Ability to predict responders based on PD - 1 or PDL - 1 expression is disappointing • Ability to predict responders based on the amount of tumor T cell infiltration is disappointing Drug development of I/O therapeutics limited by • Mouse models that do not predict human immuno - oncology • Drugs not optimized for effects on non - T cell immune cells • Drugs not show dose response curve • Unclear when dosing can be stopped: continued response long after discontinuation 5

ENUM Solution: A Differentiated PD - 1 Antibody ENUM Platform - Generated antibody C8 • May act on PD - 1 through non competitive mechanism • Does not disrupt PD - L1 interaction with PD - 1 • May Activate both Active and Innate immune systems • Translational approach leverages human biopsy profiling • Provides Basis for Rational Combinations • Opportunity to treat prior PD - 1 failure and relapse 6

ENUM C8 Reverses PD - L1 Suppression 8 C8 Antibody in Cell - Based Assays: T Cell Activation IFNg secretion (relative to control) N = 5 repetitions for each candidate/condition • PBMC - based assay to determine mAb’s ability to disrupt functional suppression by PD - L1 • Pembrolizumab used as positive control Potent T Cell Activator

ENUM C8 Does Not Disrupt The Interaction between PD - L1 and PD - 1 9 No displacement of PD - L1 by ENUM C8 • PD1 - expressing HEK293 incubated with controls (isotype, negative control; EH12.2H7, positive control) and test antibodies D4 and C8 • Cells retaining labeled PD - L1 reagent visualize with FACS • Epitope mapping and biophysical studies confirm non - competitive binding Positive control EH12.2H7 Negative control mIgG1K 388D4 244C8 Positive control EH12.2H7 Negative control mIgG1K 388D4 244C8

ENUM C8 Acts via Allosteric Mechanism 10 PD - L1 engagement results in T cell inhibition Current anti - PD1 agents disrupt PD - L1 binding to PD - 1 Hypothesis: ENUM anti - PD1: novel epitope and PD - L1 independent binding; IL - 2R autocrine activation enhanced T cell activation ENUM antibody: PD - L1 independent binding and enhanced activation via autocrine IL - 2 signaling.

ENUM C8 may have Multiple Actions in the Cancer Immunity Cycle 11 Oncology Meets Immunology: The Cancer - Immunity Cycle ; Daniel S. Chen, Ira Mellman Immunity; Volume 39, Issue 1, Pages 1 - 10 (July 2013) Cancer Immunity Cycle pembro nivo C8 Ag presentation x Priming & activation x Killing x x x 2 3 7 2 3 7 Numbers refer to steps in the cancer immunity cycle and key to data on following slides

C8 Inhibits Tumor Growth in Mouse Model with Re - constituted Human T Cell Lineages 12 Tumor size (mm 3 ) 0 3 6 10 13 17 20 24 28 0 250 500 750 1000 1250 Group 1: Vehicle Group 2: 388D4 Group 3: 244C8 Group 4: Pembrolizumab Day 95% confidence interval plotted Studies performed by The Jackson Laboratory, NSG model; *388D4 is Enumeral’s conventional anti - PD1 antibody Immuno - humanized Mouse Models of Lung Cancer 7 * • Known deficiency of model: does not reconstitute human innate immune cell lineages • C8 behaves similarly to conventional anti - PD1 antibodies • Confirms C8 function is dependent on T cells

D 4 - H C 3 + L C 1 D 4 - H C 1 + L C 3 D 4 - H C 3 + L C 3 C 8 - H C 1 + L C 1 C 8 - H C 1 + L C 3 C 8 - H C 2 + L C 1 N i v o - I g G 4 h I g G 4 - H C A 2 4 7 0 5000 10000 MLR Donor 337+23 (IFNy) I F N y ( p g / m l ) 10 ug/ml 1 ug/ml 0.1 ug/ml 0.01 ug/ml Antibody concentration Antibody concentration D 4 - H C 3 + L C 1 D 4 - H C 1 + L C 3 D 4 - H C 3 + L C 3 C 8 - H C 1 + L C 1 C 8 - H C 1 + L C 3 C 8 - H C 2 + L C 1 N i v o - I g G 4 h I g G 4 - H C A 2 4 7 0 10 20 30 40 50 % o f C D 2 5 + ( C D 4 g a t e d ) 1 ug/ml 10 ug/ml 0.1 ug/ml 0.01 ug/ml MLR Donor 337+23 (T cell activation analysis by FACS) Stimulation of IFN g production Antibody concentration D 4 - H C 3 + L C 1 D 4 - H C 1 + L C 3 D 4 - H C 3 + L C 3 C 8 - H C 1 + L C 1 C 8 - H C 1 + L C 3 C 8 - H C 2 + L C 1 N i v o - I g G 4 h I g G 4 - H C A 2 4 7 0 10 20 30 40 50 % o f C D 2 5 + ( C D 4 g a t e d ) 1 ug/ml 10 ug/ml 0.1 ug/ml 0.01 ug/ml MLR Donor 337+23 (T cell activation analysis by FACS) Stimulation of CD25 expression (high affinity IL - 2R) Nivo IgG4 D4 - 1 D4 - 2 D4 - 3 C8 - 1 C8 - 2 C8 - 3 Nivo IgG4 D4 - 1 D4 - 2 D4 - 3 C8 - 1 C8 - 2 C8 - 3 Evidence for C8 Enhancement of T - Cell Priming and Activation 13 ENUM C8 induces high affinity IL - 2R expression on activated T cells Data shown for humanized clones compared to nivolumab and IgG4 control; research - grade nivolumab used in these experiments Both ENUM antibodies C8 and D4 show enhanced activation in mixed lymphocyte reaction (dose - dependent stimulation of IFN g production) 3

ENUM C8 Elicits Cytokine Signature Consistent with Activation of Innate Immunity 14 Ex vivo cytokine production in NSCLC tumor biopsy consistent with focal activation of both innate & adaptive anti - tumor immunity B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 10000 20000 30000 40000 Lung IL-6 p g / m l B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 20 40 60 80 100 Lung IL-12 p g / m l B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 50 100 150 200 Lung IL-18 p g / m l B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 500 1000 1500 Lung TNF- p g / m l B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 500 1000 1500 2000 2500 Lung GMCSF p g / m l B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 50 100 150 200 Lung IL1- p g / m l B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 10000 20000 30000 40000 Lung IL-6 p g / m l B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 20 40 60 80 100 Lung IL-12 p g / m l B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 50 100 150 200 Lung IL-18 p g / m l B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 500 1000 1500 Lung TNF- p g / m l B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 500 1000 1500 2000 2500 Lung GMCSF p g / m l B i o e g e n d I g G 4 ( 1 0 u g / m L ) 3 8 8 D 4 - 2 ( 1 0 u g / m L ) 2 4 4 C 8 - 2 ( 1 0 u g / m L ) 0 50 100 150 200 Lung IL1- p g / m l Assays performed using lung biopsy obtained from patients undergoing staging surgeries. Trends observed in three independent experiments. 2 3 +

C8 Mechanism is CD40L - Dependent: Consistent with Linkage of Adaptive and Innate Immunity 15 Kambayashi & Laufer NRI 2014 CMV recall assay with C8 PD1 blockade ± CD40L blockade These data suggest C8 - based PD1 blockade potentiates both T cell activation ( IFNg ) & APC (IL - 12) activation 2

Summary: Rational Basis for C8 PD - 1 - dependent Enhanced Immune Activation 16 • First - generation drugs optimized for effects on T cells* but T cells do not act alone in the TME • C8 may differentially drive cross - talk between adaptive and innate immune cells in the TME via cytokine secretion • C8: Potential for differentiated effects via activation of both adaptive and innate: harder for the tumor to escape immune control *OPDIVO (nivolumab) Prescribing Information, revised October 2015; Keytruda (pembrolizumab) Prescribing Information, Oct.2 2015.; Motzer RJ et al N Engl J Med2015; 373(19):1803 - 1813; OPDIVO( nivolumab ) Prescribing Information, revised November 2015 From http://www.sciencedirect.com/science/article/pii/S0304419X14000110

TIM - 3 Program Rationale • TIM - 3 expression often correlates with PD - 1 expression in vivo • TIM - 3 may drive T cell exhaustion and adaptive resistance following PD - 1 blockade • Anti - TIM - 3 alone or in combinations with anti - PD - 1 or other immuno - oncology agents may result in enhanced responses • Anti - TIM - 3 combinations might provide responses in patients that are resistant to PD - 1 checkpoint blockade • Combinations including an anti - TIM - 3 antibody might provide responses in tumor types that have been resistant to PD - 1 checkpoint blockade 18

Overview: ENUM TIM - 3 Program • Exceptional sequence diversity of anti - TIM - 3 antibodies – Many ENUM TIM - 3 candidates result in greater T cell function than a TIM - 3 reference antibody – Implies ENUM TIM - 3 antibodies are potent – Multiple potential leads supporting drug candidate selection • Functional selection approach leveraging patient - derived biopsy – ENUM anti - TIM - 3 antibodies restore T cell function in NSCLC TILs resistant to PD - 1 blockade – Implies responsiveness in PD - 1 - resistant and/or nonresponsive tumors 19

TIM - 3 Program Diversity Supports Development of Differentiated Candidates 20 *Based on ENUM evaluation of published literature Dendrogram showing bioinformatics analysis of Vh amino acid sequence among selected ENUM anti - TIM - 3 antibodies and known patented antibodies. • TIM - 3 target complexity: multiple ligands and expression on many cell types – Diversity critical for empirical selection of appropriate lead candidate based on function in primary tumor biopsy assays • Unprecedented diversity in ENUM TIM - 3 Program*: – 124 Sequences – 42 Families • Clones from 10 different families chosen for scale - up – Represent diversity of screen – Encompass known published antibody space* – Primary biopsy assays for lead nomination ongoing

ENUM TIM3 Antibodies are Potent: CD4 T Cell Stimulation 21 TIM3 Ab 1ug/ml IFNr pg/ml 100812_E1VH3100814_F2VH1100814_G1VH7100878_F1VH7100666_D1VH72e2 mIgG1 0 100 200 300 400 500 100812_E1VH3 100814_F2VH1 100814_G1VH7 100878_F1VH7 100666_D1VH7 2e2 mIgG1 Suboptimal CD3/CD28 - based stimulation of human donor PBMCs; Purified antibodies used Enumeral anti - TIM - 3 antibodies outperform a commercial reagent reference antibody (2e2) 1 0 0 8 1 4 _ A 2 V H 2 _ 1 1 0 0 8 1 4 _ D 1 V H 5 _ 1 1 0 0 8 1 4 _ F 1 V H 3 _ 2 1 0 0 8 7 8 _ F 1 V H 7 _ 1 1 0 0 8 8 0 _ B 2 V H 7 _ 1 1 0 0 8 1 2 _ G 2 V H 1 _ 1 2 e 2 1 0 2 e 2 1 m I g G 1 0 1000 2000 3000 4000 5000 Tim-3 ab stimulate CD4 T cells I F N y ( p g / m l ) ENUM anti - TIM3 antibodies cross - react with Cynomolgus TIM3 protein.

ENUM Anti - TIM3 Antibody Activates PD - 1Blockade - Refractory NSCLC TILs Ex Vivo 22 m I g G 1 k ( 1 u g / m L ) T I M - 3 # 5 ( 1 u g / m L ) 2 4 4 c 8 ( 1 0 u g / m L ) + T i m 3 # 5 ( 1 u g / m L ) 0 5000 10000 15000 I F N - ( p g / m L ) Isotype control Tumor WD - 22 NSCLC tumor WD - 22 was refractory to PD - 1 blockade in ex vivo TIL activation assays; ENUM anti - TIM3 antibody 100814G1 used in this experiment Primary NSCLC biopsy assays

Enumeral TIM3 Program Summary • Identification of multiple potent potential lead candidates • Evaluation of NSCLC tumors demonstrates: – Heterogeneity of response to immune checkpoint blockade • TIM - 3 blockade can activate PD - 1 blockade - refractory TILs ex vivo • Human biopsy - based rationale for selection of lead candidates and development of combinatorial immunotherapy 23

Other Enumeral Pipeline Programs BMS Immutep 24 GSK • ENUM LAG - 3 Antibodies To - Date: Broad Diversity – 102 Sequences – 40 Families – Encompass known published antibody space* • Other Screening Programs: – TIGIT – VISTA – OX40 – Others not disclosed Dendrogram showing bioinformatics analysis of Vh amino acid sequence among ENUM anti - LAG - 3 antibodies and known patented antibodies. *Based on ENUM evaluation of published literature

Clinical Opportunity for Next Generation Checkpoint Blockers 26 Non responders Responders Opportunity: new products to treat prior therapy failures and relapse Resistant or refractory patients Goal: improve aggregate response rates Eligible patients

Enumeral Pipeline 27 ENUM 244C8 • Novel allosteric mechanism of binding leads to enhanced activity in vitro • Binding: differentiated epitope • Novel composition of matter • Potential mechanism of action through coordinated activation of adaptive and innate immunity TIM - 3 • Broad diversity of antibodies • Patient biopsy data supports rational combination: TIM - 3 + PD - 1 • Ex vivo data supports potential for monotherapy in PD - 1 - refractory tumors PD - 1 Targeted Antibodies TIM - 3 Targeted Antibodies ENUM 388D4 • Similar activity to approved PD - 1 inhibitors in vitro (“conventional”) • Represents minimal investment with potential for business upside through out - licensing

ENUM C8, D4 and other Pipeline Antibodies have Potential to Address Unmet Clinical Need 28 Checkpoint blockade improves efficacy of other modalities Potential to treat relapse and refractory PD - 1 patients: expand into open label registration trial Checkpoint combination inhibition may treat resistant tumor types Lead allosteric antibody: differentiated mechanism of action Fast to market development strategy Clinical Trial Plans Support Testing of Multiple Combinations IND TIM 3 Phase 1 388D4 Phase 1 IND TIM3 244C8 388D4 Vaccine or Targeted Rx 388D4 TIM3 244C8 Phase 2 : Monotherapy IND 244C8 Phase 1

Proprietary Platform Drives Discovery and Development Advantage 30 84,672 50µm microwells • Proprietary broadly enabling “Immune system on a chip” platform • Immuno - oncology still a “black box” – Requires mechanistically differentiated assets for entry – Requires translational biology insights to gain competitive advantage Measures key parameters of response of individual immune cells from patient samples to drug candidates Uniquely identifies rare immune cells critical to responses for mechanistic differentiation Recover cells of interest and gene sequences encoding natural antibodies and T cell receptors

Primary Human Tumor Biopsy Approach Drives Translational Antibody Selection 31 function phenotype genomics Patient - centric integrated single cell functional immune profiling of antibody modulation of cytokine secretion and receptor expression Patient biopsy Enumeral has developed a translational cancer biology platform that uses patient - derived biopsy After Tsioris et al 2015 The Human Approach TM enables our scientists to validate targets, screen and select therapeutic candidates that we believe have potential to be best - in - class therapies

Enumeral Research Activities 32 Antibody Discovery Target Discovery and Validation In Vitro Pharmacology Tumor Profiling, TCR, Genomics Cell Based Assays Lead discovery Translational & Clinical Sciences Clinical strategy & ITT cohort identification Target ID & Nomination Development Candidate

Diversity leads to Differentiated Antibodies • Enumeral antibody discovery results in exceptional diversity* • Drives empirical function - based approach: diversity is key to identifying potential ‘best - in - class’ candidates using primary biopsy validation • Potential for strong IP position OPDIVO® KEYTRUDA® Pidilizumab 392C5 246A10 413D2 413E1 244C8 388D4 *Based on ENUM evaluation of published literature Example: dendrogram representing heavy chain AA sequences from anti - PD - 1 program; N= 159 sequences clustering into 28 families of antibodies Novel Anti - PD - 1 Antibody 244C8 33

Strong Intellectual Property Position • Exclusive worldwide license with MIT/Harvard for platform technology – 6 issued patents in US and 32 issued in international jurisdictions – 26 pending patent applications (US & International) • Patents covering compositions of matter and methods – Applications pending or in preparation covering compositions of matter, methods of making, and methods of treating disease, for Enumeral discovered antibodies – Application pending for methods for cellular response profiling • Freedom to operate – Ability to navigate crowded patent landscape – Extensive searches and detailed analyses conducted on ongoing basis – Formal opinions of outside counsel obtained, where appropriate 34

Collaborators Provide External Recognition of Differentiated Approach • MERCK : collaboration with a leading immuno - oncology pharmaceutical company – Focused on using Enumeral's platform to interrogate the tumor microenvironment in colorectal cancer tissues to identify functional cellular responses to therapies being developed by Merck – R&D funding and undisclosed milestone payments – Merck has exclusive rights to data related to its proprietary compounds – ENUM recently achieved first milestone in the collaboration • NCI: awarded Phase 2 contract for ~$1 million over two years – Automation of human tissue immuno - oncology profiling – Opens door to broader pipeline and potentially accelerated development – Collaboration with leading scientists: 36 – Jedd Wolchok’s group at MSKCC − genetic basis for response to checkpoint inhibitors and novel immunotherapeutics – Doug Kwon’s group at MGH/ Ragon Institute − pioneering techniques for single cell immune cell analysis in biopsy

Strategic Collaboration with MD Anderson Cancer Center • Goal to Discover and Develop Novel Antibodies Against Specified Immunotherapy Targets – Utilizes Enumeral’s antibody discovery and patient - centric immune profiling platform – Leverages MD Anderson’s preclinical and development expertise and infrastructure – Collaboration with Oncology Research for Biologics and Immunotherapy Translation ( ORBIT ), a translational research platform of MD Anderson’s Moon Shots Program – Enumeral and MD Anderson will jointly fund research and development activities, and will share net income from product sales or any payments associated with third party partnering – Targets have not been disclosed • Impact on Potential Future Collaborations – Goal is for ENUM and MDACC to jointly out - license following clinical proof of concept, where a partner could step in to continue development 37

Experienced Leadership Team 38 John J. Rydzewski Executive Chairman, Co - Founder, Director Arthur H. Tinkelenberg, Ph.D. President & CEO, Co - Founder, Director Cokey Nguyen, Ph.D. Vice President, Research & Development Isabel Chiu, Ph.D. Vice President, Translational & Clinical Sciences Kevin G. Sarney Vice President, Finance, & Chief Accounting Officer Derek Brand Vice President, Business Development Matthew A. Ebert General Counsel Gary L. Creason , Ph.D. Vice President, Intellectual Property John J. Rydzewski Executive Chairman, Co - Founder, Director Arthur H. Tinkelenberg, Ph.D. President & CEO, Co - Founder, Director Cokey Nguyen, Ph.D. Vice President, Research & Development Isabel Chiu, Ph.D. Vice President, Translational & Clinical Sciences Kevin G. Sarney Vice President, Finance, & Chief Accounting Officer Derek Brand Vice President, Business Development Matthew A. Ebert General Counsel Gary L. Creason , Ph.D. Vice President, Intellectual Property John J. Rydzewski Executive Chairman, Co - Founder, Director Arthur H. Tinkelenberg, Ph.D. President & CEO, Co - Founder, Director Cokey Nguyen, Ph.D. Vice President, Research & Development Isabel Chiu, Ph.D. Vice President, Translational & Clinical Sciences Kevin G. Sarney Vice President, Finance, & Chief Accounting Officer Derek Brand Vice President, Business Development Matthew A. Ebert General Counsel Gary L. Creason , Ph.D. Vice President, Intellectual Property

Non - Management Directors 39 Barry Buckland, Ph.D. Co - Founder; Chairman, Scientific Advisory Board Robert J. Easton Allan Rothstein Paul J. Sekhri Robert L. Van Nostrand

THE POWER of HUMAN™

Enumeral Biomedical (ENUM) 424B3Prospectus supplement