60 patents
Utility
Methods of sperm cell sensing utilizing a semiconductor detector and cytometer apparatus
2 Jan 24
A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry.
Frederick Savage, Glenn J. Szejna, Zheng Xia
Filed: 1 Feb 23
Utility
Cryopreserving Ungulate Embryos
21 Dec 23
Technologies for cryopreserving ungulate embryos for implantation into recipient females are described.
Bruno Valente Sanches
Filed: 27 Jul 23
Utility
Apparatus and Method for Cell Kill Confirmation
16 Nov 23
A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.
Frederick Savage, David Appleyard, Zheng Xia, Matthew D. Ebersole, Daniel McAda
Filed: 14 Jul 23
Utility
Compositions and Methods for Enhancing Sperm Cell Quality
26 Oct 23
The present disclosure relates generally to potassium channel blocker compounds and compositions thereof; and methods for improving reproductive cell viability and quality, during and/or after one or more of staining, freezing, thawing, cell sample enrichment, packaging, or in vitro fertilization using the potassium channel blocker compounds.
Elon Roti-Roti, Xiao Peng, Rebecca Winters, Ashley Schroeder
Filed: 29 Jul 22
Utility
Microfluidic system and method with focused energy apparatus
24 Oct 23
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
David Appleyard, Jeff Betthauser, Marjorie Faust, John Larsen, Guocheng Shao, Zheng Xia, Yu Zhou
Filed: 19 Feb 19
Utility
Cryopreserving ungulate embryos
24 Oct 23
Technologies for cryopreserving ungulate embryos for implantation into recipient females are described.
Bruno Valente Sanches
Filed: 2 Mar 22
Utility
Microfluidic System and Method with Focused Energy Apparatus
14 Sep 23
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
David Appleyard, Jeff Betthauser, Marjorie Faust, John Larsen, Guocheng Shao, Zheng Xia, Yu Zhou
Filed: 16 May 23
Utility
Apparatus and method for cell kill confirmation
12 Sep 23
A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.
Frederick Savage, David Appleyard, Zheng Xia, Matthew Ebersole, Daniel McAda
Filed: 13 Jul 22
Utility
Single-sheath Microfluidic Chip
17 Aug 23
Microfluidic devices and methods for focusing components in a fluid sample are described herein.
Zheng Xia, Gopakumar Kamalakshakurup
Filed: 31 Mar 23
Utility
Transition cow index
4 Jul 23
Applicants have identified that three critical phenotypic/genetic measures are highly correlated with transition period health and may be used in selection and breeding protocols and/or in combination with traditional breeding and marker assisted selection methods to improve predictability of transition period health.
Katrina Dattilo, Yalda Zare, Gabriela Marquez Betz, Kaleena Stephan, Ryan Starkenburg, Cristian Vergara
Filed: 19 Jun 19
Utility
Microfluidic system and method with focused energy apparatus
2 May 23
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
David Appleyard, Jeff Betthauser, Marjorie Faust, John Larsen, Guocheng Shao, Zheng Xia, Yu Zhou
Filed: 28 Jun 22
Utility
Single-sheath microfluidic chip
18 Apr 23
Microfluidic devices and methods for focusing components in a fluid sample are described herein.
Zheng Xia, Gopakumar Kamalakshakurup
Filed: 13 Jan 20
Utility
Kill Event Optimization
16 Mar 23
A method of choosing which undesired cell to destroy in a multi-cell fluorescent event includes detecting fluorescence of cells, converting photons detected in the fluorescence into an analog voltage output signal, and identifying at least two discernable peaks associated with the cells.
Frederick Savage, Matthew D. Ebersole, Daniel McAda, David Appleyard, Zheng Xia
Filed: 28 Oct 22
Utility
Methods of sperm cell sensing utilizing an avalanche photodiode and cytometer apparatus
7 Mar 23
A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry.
Frederick Savage, Glenn J. Szejna, Zheng Xia
Filed: 25 Apr 22
Utility
Microfluidic Chip
2 Feb 23
A microfluidic chip orients and isolates components in a sample fluid mixture by two step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods.
Zheng Xia, Yu Zhou, John Larsen, Guocheng Shao, Shane Peterson, Marjorie Faust
Filed: 29 Sep 22
Utility
Microfluidic System and Method with Focused Energy Apparatus
8 Dec 22
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
David Appleyard, Jeff Betthauser, Marjorie Faust, John Larsen, Guocheng Shao, Zheng Xia, Yu Zhou
Filed: 28 Jun 22
Utility
Microfluidic chip
29 Nov 22
A microfluidic chip orients and isolates components in a sample fluid mixture by two step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods.
Zheng Xia, Yu Zhou, John Larsen, Guocheng Shao, Shane Peterson, Marjorie Faust
Filed: 16 Aug 21
Utility
Kill event optimization
29 Nov 22
A method of choosing which undesired cell to destroy in a multi-cell fluorescent event includes detecting fluorescence of cells, converting photons detected in the fluorescence into an analog voltage output signal, and identifying at least two discernable peaks associated with the cells.
Frederick Savage, Matthew Ebersole, Daniel McAda, David Appleyard, Zheng Xia
Filed: 29 May 20
Utility
Apparatus and Method for Cell Kill Confirmation
3 Nov 22
A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.
Frederick Savage, David Appleyard, Zheng Xia, Matthew Ebersole, Daniel McAda
Filed: 13 Jul 22
Utility
Cell Differentiation Based on Multi-directional Light from a Microfluidic Chip
22 Sep 22
Disclosed is an approach to differentiating between different particle types in samples flowing through microfluidics chips.
David Appleyard, Daniel McAda, Zheng Xia, James Maxwell Schiller, Frederick Savage, John Walker Rupel, II, Timothy Miller, Alec Fisher
Filed: 16 Mar 22