34 patents
Utility
Cryopreserving Ungulate Embryos
21 Dec 23
Technologies for cryopreserving ungulate embryos for implantation into recipient females are described.
Bruno Valente Sanches
Filed: 27 Jul 23
Utility
Apparatus and Method for Cell Kill Confirmation
16 Nov 23
A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.
Frederick Savage, David Appleyard, Zheng Xia, Matthew D. Ebersole, Daniel McAda
Filed: 14 Jul 23
Utility
hx3yee2njnob6gauqhoe6vl40
26 Oct 23
The present disclosure relates generally to potassium channel blocker compounds and compositions thereof; and methods for improving reproductive cell viability and quality, during and/or after one or more of staining, freezing, thawing, cell sample enrichment, packaging, or in vitro fertilization using the potassium channel blocker compounds.
Elon Roti-Roti, Xiao Peng, Rebecca Winters, Ashley Schroeder
Filed: 29 Jul 22
Utility
8x1fo5irrgs7af9i1e82c1ocdnp1wu94ay69k5wec5bpcdy1vovsr54kq
14 Sep 23
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
David Appleyard, Jeff Betthauser, Marjorie Faust, John Larsen, Guocheng Shao, Zheng Xia, Yu Zhou
Filed: 16 May 23
Utility
2qi8076tnq93t9uk1o1ro
17 Aug 23
Microfluidic devices and methods for focusing components in a fluid sample are described herein.
Zheng Xia, Gopakumar Kamalakshakurup
Filed: 31 Mar 23
Utility
ykxicy5vqi4lqluicwa4sknmtbs1ga736brxzdchm 6fu3e2v7fhfj
16 Mar 23
A method of choosing which undesired cell to destroy in a multi-cell fluorescent event includes detecting fluorescence of cells, converting photons detected in the fluorescence into an analog voltage output signal, and identifying at least two discernable peaks associated with the cells.
Frederick Savage, Matthew D. Ebersole, Daniel McAda, David Appleyard, Zheng Xia
Filed: 28 Oct 22
Utility
nelfj2ghx4bfzxc5rbisrglxana6g90qr5edkj18d
2 Feb 23
A microfluidic chip orients and isolates components in a sample fluid mixture by two step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods.
Zheng Xia, Yu Zhou, John Larsen, Guocheng Shao, Shane Peterson, Marjorie Faust
Filed: 29 Sep 22
Utility
y5nbwyocq2w8rmj363g5wot3g
8 Dec 22
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
David Appleyard, Jeff Betthauser, Marjorie Faust, John Larsen, Guocheng Shao, Zheng Xia, Yu Zhou
Filed: 28 Jun 22
Utility
a5efot3amijtybikszl8ptwergkiv3pld17n84ntmpeh4wfhvgkv qbs2g7
3 Nov 22
A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.
Frederick Savage, David Appleyard, Zheng Xia, Matthew Ebersole, Daniel McAda
Filed: 13 Jul 22
Utility
choxr6re5pubp4wxlf7ycqgiwa0dpazx5rwwau7tkhwcmds
22 Sep 22
Disclosed is an approach to differentiating between different particle types in samples flowing through microfluidics chips.
David Appleyard, Daniel McAda, Zheng Xia, James Maxwell Schiller, Frederick Savage, John Walker Rupel, II, Timothy Miller, Alec Fisher
Filed: 16 Mar 22
Utility
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4 Aug 22
A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry.
Frederick Savage, Glenn J. Szejna, Zheng Xia
Filed: 25 Apr 22
Utility
g9bdroighn4uhlthqp7nwwljsu 9m3671q6k
21 Jul 22
A microfluidic system configured to focus particles suspended in a fluid.
Gerson Aguirre, Zheng Xia, Gopakumar Kamalakshakurup
Filed: 5 Apr 22
Utility
mv092ns8kn77ccsrxxvf568zc3fxglniv1yhmgk
16 Jun 22
Technologies for cryopreserving ungulate embryos for implantation into recipient females are described.
Bruno Valente SANCHES
Filed: 2 Mar 22
Utility
qlmpm7ebcunpe0hqlkz0667ic35sy5hi0jseks3c2cj1mz9dywn9
26 May 22
Modular flow cytometry systems and methods for processing samples are described herein.
Gary Klas, Daniel McAda, Zheng Xia, Matthew Ebersole, Brian Lena, David Appleyard, Richard Lu, Scott Buckley, Mark Abermoske
Filed: 12 Nov 21
Utility
lfwkeej8uzfcg8g8u73b c2lincfg6dal5tnoaw3je6r863tokboe1
21 Apr 22
The present disclosure relates generally to methods for using porcine sex-sorted sperm cells for the efficient dissemination of desirable traits in multi-level swine production systems.
Matthew CULBERTSON, Daniel HAMILTON, Matthew EBERSOLE, William HERRING
Filed: 14 Oct 21
Utility
qwtflqux9fxf4rj873ou1u4 t1oj7eygxti4hfyyq8bp1m1rwafw
21 Apr 22
Methods and systems for processing semen samples from straws for determining genetic identity, testing the purity of the sample, detecting errors or contamination, calculating an amount of contamination, and determining the identity of the contaminant.
Jennifer Dzelil, Matthew Campbell, Steve Rounsley, Patrice Linel
Filed: 21 Oct 21
Utility
lqpauxio5rpn6xywzlxuyvl3
31 Mar 22
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation; and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
David Appleyard, Jeff Betthauser, Marjorie Faust, John Larsen, Guocheng Shao, Zheng Xia, Yu Zhou
Filed: 7 Oct 21
Utility
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3 Mar 22
Systems and methods are provided for identification and discrimination of subpopulations within a mixture of particles.
Jim HARRINGTON, Dan ANTONUK, Fred SAVAGE, David APPLEYARD, Matthew EBERSOLE
Filed: 21 Dec 18
Utility
ri3a8t30dw8jzreu8unvhmn6v3399sv702xku4 3bkekwf0bipd2z
27 Jan 22
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
David Appleyard, Jeff Betthauser, Marjorie Faust, John Larsen, Guocheng Shao, Zheng Xia, Yu Zhou
Filed: 7 Oct 21
Utility
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23 Dec 21
The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing.
Daniel Mueth, Joseph Plewa, Jessica Shireman, Amy Anderson, Lewis Gruber, Neil Rosenbaum
Filed: 27 Aug 21