154 patents
Utility
Methods and Devices for Measuring Duplex Unwinding or Strand Exchange Efficiency
18 Jan 24
Provided herein are quantitative assays to evaluate duplex unwinding efficiency or strand exchange efficiency during isothermal amplification.
Yanhong Tong, Yali Sun, Thomas Perroud
Filed: 12 Jul 22
Utility
Electrophoresis-mediated Characterization of Dna Content of Adeno-associated Virus Capsids
7 Dec 23
According to aspects of methods of characterizing a population of particles putatively containing recombinant nucleic acids in a fluid sample of the present disclosure, includes extracting nucleic acids from the particles, labeling the extracted recombinant nucleic acids, separating the labeled nucleic acids by size, and comparing the separated labeled nucleic acids with a standard to determine one or more of: 1) a ratio of full viral particles to empty viral particles in the fluid sample, 2) a ratio of full viral particles or empty viral particles to partially full viral particles, and 3) a ratio of viral particles containing an intact recombinant genome to viral particles containing an incomplete recombinant genome, thereby characterizing the population of particles in the fluid sample.
James White, Brendan Curran, Lloyd Bwanali, James Atwood, Shreyas Shah, Dipti Mehta, Menel Ben Frej
Filed: 31 May 23
Utility
Pipetting Apparatus and Methods
16 Nov 23
An automated pipetting system includes a pipettor.
Thomas Perroud, Thomas Rawlins, Hans-Juergen Tiedtke, Kai Hassler
Filed: 11 May 22
Utility
Compositions and Methods Relating to Loop Mediated Isothermal Amplification (Lamp)
16 Nov 23
Methods for detection of a target nucleic acid in a sample are provided according to aspects of the present disclosure which include: providing a reaction mixture comprising a LAMP assay primer set specific for the target nucleic acid, magnesium, dNTPs, a reaction buffer, a DNA polymerase, and a sample to be tested for presence of the target nucleic acid, wherein the LAMP assay primer set comprises a forward inner primer (FIP), a backward inner primer (BIP), a forward outer primer (F3) and a backward outer primer (B3), wherein the FIP and BIP are present in a non-equal ratio such that molar concentration of BIP is highly skewed relative to molar concentration of FIP.
Eva May Ng, Matthew Hooper, Nicolas M. Kosa, Erik Breitenfeldt
Filed: 16 May 22
Utility
Pipetting Apparatus and Methods
16 Nov 23
An automated pipetting system includes a pipettor.
Thomas Perroud, Thomas Rawlins, Hans-Juergen Tiedtke, Kai Hassler
Filed: 26 May 22
Utility
Methods, Compositions, and Kits for Inhibiting Formation of Adapter Dimers
12 Oct 23
Methods of reducing adapter-dimers in a sequencing library are provided according to aspects of the present disclosure, including: hybridizing excess 3′ adapters to blocker oligonucleotides forming a hybridized complex, such that the excess 3′ adapters are unavailable to form adapter-dimers, the hybridized complex including: a first blocker oligonucleotide 3′ terminus adjacent to an adenylated nucleotide at the 5′ terminus of a first unligated 3′ adapter and a second blocker oligonucleotide 3′ terminus adjacent to an adenylated nucleotide at the 5′ terminus of a second unligated 3′ adapter, wherein the first blocker oligonucleotide has a 5′ portion complementary to and hybridized to the first unligated 3′ adapter, the second blocker oligonucleotide has a 5′ portion complementary to and hybridized to the second unligated 3′ adapter, and the first blocker oligonucleotide has a 3′ portion complementary to, and hybridized to, a 3′ portion of the second blocker oligonucleotide.
Kevin D. Allen, Kerry Gunning, Allyson LeBas, Eric Castro
Filed: 24 Mar 23
Utility
Microfluidic Chip and Electrical Interface for Microchip Electrophoresis
28 Sep 23
A microfluidic system may include a microfluidic chip having a non-conductive substrate and wells connected in common to a microfluidic channel within the non-conductive substrate.
Allen Curtis Dennison, Vladimir Krasheninin, Stephen J. Allen, Thomas Rawlins, Richard Raymond Harazin, Michael W. Belobraydich, Narendra Vaidya, Shreyas Milankumar Shah, Thomas John Martiska
Filed: 22 Mar 22
Utility
Methods for Separating and Detecting Double-stranded and Single-stranded Ribonucleic Acid (Rna)
7 Sep 23
Provided herein are capillary electrophoresis methods for separating and detecting double-stranded ribonucleic acid (dsRNA) contaminants in samples of single-stranded ribonucleic acids (ssRNA) such as RNA therapies (e.g., mRNA vaccines).
Lloyd Bwanali, Guangnan Meng, Shreyas Shah
Filed: 28 Feb 23
Utility
Compositions and Methods for Performing Magnetibuoyant Separations
31 Aug 23
The methods of the invention employ targeted magnetic particles, preferably targeted nanomagnetic particles, and targeted buoyant particles such as buoyant microparticles and microbubbles.
DHANESH GOHEL, HONG ZHANG, JOHN RANSOM
Filed: 19 Jan 23
Utility
Toroidal Ion Trap
10 Aug 23
Provided are improved toroidal ion traps and methods of design of such ion traps.
Stephen D. Lammert, Atanu K. Mohanty, Shawn Johnson, Robert Jackson
Filed: 3 Feb 23
Utility
Method for Producing Genetically Modified Cells
27 Jul 23
A method for producing genetically engineered immune cells, e.g.
John Lambourne, Immacolata Porreca
Filed: 9 Jul 21
Utility
CorrectedMethod and reagents for diagnosing Membranous Nephropathy
6 Jul 23
Provide are a diagnostically useful carrier coated with a recombinant polypeptide comprising SEQ ID NO: 1, an isolated autoantibody binding specifically to a polypeptide having SEQ ID NO: 1, a kit comprising the carrier, a method for the diagnosis of a membranous nephropathy (MN) that includes detecting the presence or absence of an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1 in a liquid sample comprising antibodies from a subject, a use of an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1 or a polypeptide comprising SEQ ID NO: 1, and a aqueous solution comprising an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1.
Inga-Madeleine DETTMANN, Swantje MINDORF, Christian PROBST
Filed: 2 Dec 21
Utility
Curved Ion Guides and Related Systems and Methods
6 Jul 23
An ion guide includes a plurality of lenses arranged in series along a curved central axis.
Shawn Johnson, Edgar Lee, Hamid R. Badiei
Filed: 5 Jan 23
Utility
Method for Filtering a Sensor Signal and Device for Controlling an Actuator by Filtering a Sensor Signal
29 Jun 23
A method according to the invention is proposed for filtering a sensor signal, said method comprising various steps.
Felix AHLBORG, Arne VON DRATHEN, Siegfried WESSEL
Filed: 20 Dec 22
Utility
Rna Scaffolds
29 Jun 23
The present invention discloses an RNA scaffold comprising a tracrRNA; and a recruiting RNA motif with an extension sequence for targeted gene editing and related uses.
MICHAEL RUSHTON, MICHAEL ANBAR, KEVIN HEMPHILL
Filed: 9 Jul 21
Utility
Stable Nanomagnetic Particle Dispersions
25 May 23
Processes and compositions are described for preparing new, colloidally stable, coated nanomagnetic particles useful for both in-vitro and in-vivo biomedical applications, including cell targeting and capturing cells, microorganisms, and cellular organelles or entities such as exosomes.
DHANESH GOHEL, HONG ZHANG, JOHN RANSOM
Filed: 19 Jan 23
Utility
Nir to Swir Fluorescent Compounds for Imaging and Detection
25 May 23
This disclosure provides a family of compounds that absorb and fluoresce in the short wave infrared region (SWIR, optionally 1000 nm to 1300 nm), including hydrophilic compounds that exhibit absorption and emission spectral profiles in aqueous solutions substantially similar to those observed in organic solvents such as methanol or DMSO.
Nara Narayanan, Kevin Groves, Guojie Ho
Filed: 7 Dec 22
Utility
Deflectors for Ion Beams and Mass Spectrometry Systems Comprising the Same
25 May 23
Provided are ion detectors and systems that may employ such ion detectors such as mass spectrometers and other instruments.
Tsung-Chi Chen, Hamid Badiei, Thomas White, Sergey Rakov, Adam J. Patkin
Filed: 17 Nov 22
Utility
Compositions and Methods for Nucleic Acid Normalization
25 May 23
Methods, compositions, and kits of the present disclosure for normalizing a mass amount of nucleic acids in each of a plurality of test samples are provided, for use in methods of nucleic acid analysis, such that substantially equal amounts of nucleic acids are present in the samples to be analyzed.
William Law, Kerry Gunning, Aracele Martinez-Mendez
Filed: 23 Nov 22
Utility
Three-Dimensional Luminescence Imaging
25 May 23
Systems, apparatuses, and methods are described for 3D luminescence imaging, by identifying a preferred optical pair and optimizing a scanned image using the preferred optical pair.
Joshua Kempner, Jorge Ripoll
Filed: 23 Nov 21