112 patents
Page 2 of 6
Utility
Methods and compositions for loading of polymerase complexes
14 Feb 23
The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions.
Sassan Sheikholeslami, Michael W. Hunkapiller, Natasha Popovich, Lei Sun, Erik Miller, Satwik Kamtekar
Filed: 23 Jun 20
Utility
Method of chemically modifying plastic surfaces
14 Feb 23
Disclosed herein are finished products, methods, compositions and kits for derivatizing plastic (e.g., “polymer”) surfaces in a manner that renders the surfaces appropriate for various downstream applications.
Corey M. Dambacher
Filed: 17 Jul 18
Utility
Analytic device comprising a substrate, nanometer-scale wells, and shallow waveguide optically coupled to a deep waveguide
24 Jan 23
This invention provides devices for use in various analytical applications including single-molecule analytical reactions.
Cheng Frank Zhong, Paul Lundquist, Mathieu Foquet, Jonas Korlach, Hovig Bayandorian
Filed: 16 Mar 21
Utility
Methods and compositions for delivery of molecule and complexes to reaction sites
20 Dec 22
The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites.
Stephen Turner
Filed: 9 Jun 20
Utility
Fluidic apparatus and methods useful for chemical and biological reactions
22 Nov 22
Provided herein is a valve manifold comprising (a) an elastomer sheet attached to a plurality of magnetic pistons, wherein the magnetic pistons project from a first side of the elastomer sheet; (b) a foot component comprising a first surface and a plurality of shafts that orthogonally pass through the first surface; and (c) a body component comprising a second surface, a groove that laterally passes along the second surface, and a plurality of reservoir channels that orthogonally pass through the second surface, wherein the elastomer sheet is compressed between the foot component and the body component.
Christopher Kilcoin, Kristin Dills, Rebecca McGinley
Filed: 30 Jun 20
Utility
Nucleic acid sequencing methods and systems
15 Nov 22
The present disclosure provides compositions, methods and systems for sequencing a template nucleic acid using a polymerase based, nucleic acid binding reaction involving examination of the interaction between a polymerase and template nucleic acid in the presence of one or more unlabeled nucleotides.
Kandaswamy Vijayan, Eugene Tu, Mark A. Bernard
Filed: 26 Jul 18
Utility
Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
15 Nov 22
A method for sequencing a population of nucleic acids, which includes (a) binding the population of nucleic acids with a fractionally labeled mixture of nucleotides, thereby forming a fractionally labeled population of nucleic acids, wherein the mixture includes nucleotide cognates for a common base type in the templates, and wherein a fraction of the nucleotide cognates for the common base type in the mixture are exogenously labeled nucleotides that produce a signal that is not produced by other nucleotide cognates for the common base type in the mixture; (b) detecting the signal from the fractionally labeled population of nucleic acids; and (c) repeating (a) and (b) using a second mixture of the fractionally labeled nucleotides, wherein the fraction of the exogenously labeled nucleotides is higher in the second mixture.
Min-Jui Richard Shen
Filed: 6 Feb 20
Utility
Distinguishing sequences by detecting polymerase dissociation
8 Nov 22
A method for determining the presence of an allele, including (a) binding a polymerase to a double stranded nucleic acid that includes a primer hybridized to a template, the template including a first allele of a locus; (b) adding a nucleotide to the primer via catalytic activity of the polymerase, thereby producing an extended nucleic acid; (c) dissociating the polymerase from the extended nucleic acid; (d) detecting dissociation of the polymerase from the extended nucleic acid; and (e) comparing the dissociation of the polymerase from the extended nucleic acid to dissociation of the polymerase from a second double stranded nucleic acid, the second double stranded nucleic acid including a primer hybridized to the same position of the locus as the primer of the extended nucleic acid.
Denis Malyshev, Sean Stromberg
Filed: 14 Feb 18
Utility
Arrays of integrated analytical devices
11 Oct 22
Arrays of integrated analytical devices and their methods for production are provided.
Annette Grot, Ravi Saxena, Paul Lundquist
Filed: 7 Dec 20
Utility
Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
11 Oct 22
Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided.
Lubomir Sebo, Gene Shen, Stephen Yue, Honey Osuna, Yuri Lapin, Louis Brogley, Andrei Fedorov
Filed: 28 Aug 20
Utility
Methods and apparatus that increase sequencing-by-binding efficiency
20 Sep 22
A method of determining a nucleic acid sequence that includes steps of: (a) contacting a primed template nucleic acid with a series of mixtures for forming ternary complexes, wherein each of the mixtures includes a polymerase and nucleotide cognates for at least two different base types suspected of being present at the next template position of the template nucleic acid; (b) monitoring the next template position for ternary complexes formed by the series of mixtures, wherein a signal state indicates presence or absence of ternary complex formed at the next template position by each individual mixture, thereby determining a series of signal states that encodes a base call for the next template position; and (c) decoding the series of signal states to distinguish a correct base call for the next template position from an error in the base call.
Sean Stromberg, John Vieceli, Kandaswamy Vijayan, Arnold Oliphant
Filed: 9 Apr 20
Utility
Serial formation of ternary complex species
23 Aug 22
A method for identifying a nucleotide in a primed template nucleic acid, including the steps of (a) providing a vessel having a primed template nucleic acid, polymerase and a nucleotide cognate of a first base type; (b) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the first base type bound at a base position of the primed template nucleic acid; (c) delivering a nucleotide cognate of a second base type to the vessel, whereby the vessel retains the primed template nucleic acid and the polymerase from step (b); (d) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the second base type bound at the base position of the primed template nucleic acid; and (e) identifying the type of nucleotide at the base position of the primed template nucleic acid.
Brittany A. Rohrman, Denis Malyshev, Morassa Mohseni Middleton, Arnold Oliphant
Filed: 22 Jul 19
Utility
Systems, devices, and methods for improved optical waveguide transmission and alignment
16 Aug 22
Provided herein are systems, devices, and methods for improved optical waveguide transmission and alignment in an analytical system.
Alexey Kuritsyn, Mark Mcdonald, Aaron Rulison, Russell Berman
Filed: 15 Mar 21
Utility
Integrated illumination of optical analytical devices
12 Jul 22
Optical analytical devices and their methods of use are provided.
Paul Lundquist, Stephen Turner
Filed: 24 Feb 20
Utility
Recombinant polymerases with increased phototolerance
5 Jul 22
Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing.
Satwik Kamtekar, Lei Jia, Erik Miller
Filed: 18 Sep 20
Utility
Synthetic nucleic acids having non-natural structures
5 Jul 22
Nucleic acid compositions having nucleotide sequences that do not occur in nature are provided.
Arnold Oliphant, James Yu
Filed: 16 Jul 20
Utility
Modified nucleotide reagents
14 Jun 22
Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided.
Gene Shen, Stephen Yue, Lubomir Sebo, Louis Brogley
Filed: 13 Jun 19
Utility
Increased signal to noise in nucleic acid sequencing
24 May 22
A method for identifying a nucleotide in a template nucleic acid by (a) providing a plurality of primer-template nucleic acid hybrids, wherein the primers have an extendable 3′ end; (b) contacting the plurality with: (i) blocked nucleotides to produce a first subset of the primer-template nucleic acid hybrids that include a blocked nucleotide at the 3′ end, and (ii) a ternary complex inhibitor to produce a second subset of the primer-template nucleic acid hybrids that include a ternary complex inhibitor; (c) forming ternary complexes that each include a polymerase, a primer-template nucleic acid hybrid of the first subset, and a cognate nucleotide; and (d) detecting the ternary complexes, thereby identifying a nucleotide in the template nucleic acid.
Chad Fleischer, Denis Malyshev
Filed: 12 Apr 19
Utility
Methods and compositions for delivery of molecules and complexes to reaction sites
17 May 22
The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites.
Jaime Juan Benitez-Marzan, Natasha Popovich, Lei Sun
Filed: 28 Jun 19
Utility
Methods of quantifying target nucleic acids and identifying sequence variants
10 May 22
The present disclosure provides compositions, methods and systems for quantifying target sequences and identifying target sequence variants.
Yi Zhang, Steven Brentano, Eugene Tu, Kandaswamy Vijayan
Filed: 6 Apr 17